B2O3-SiO2-Na2O缓释抑菌剂的合成及性能表征

doi:10.15541/jim20160395

摘要/Abstract

摘要：

硼酸盐具有抑菌和抑藻性能, 研究采用高温熔融法合成了以硼为抑菌因子的B₂O₃-SiO₂-Na₂O缓释抑菌剂。运用X射线衍射分析(XRD)、扫描电镜(SEM)及红外光谱(FTIR)表征抑菌剂的理化性能, 采用电感耦合等离子体发射光谱仪(ICP-AES)测定缓释液中硼元素的浓度并分析抑菌剂的缓释特性, 分别以大肠杆菌和金黄色葡萄球菌为受试细菌, 以抑菌圈和抑菌率为指标评价药剂的抑菌效果。结果表明, 抑菌剂均呈无定形非晶形态, 内部[BO₃]和[BO₄]基团的含量不只与B₂O₃有关, 也受SiO₂的影响; 随着B₂O₃含量的降低, 抑菌剂表面结构逐渐致密, 缓释速率降低。随着缓释液中硼浓度的升高, 抑菌剂的抑菌作用增强, 且对金黄色葡萄球菌的抑制效果优于大肠杆菌。其中B₂O₃含量为55wt%的B₂O₃-SiO₂-Na₂O缓释抑菌剂的抑菌效果最佳, 这为硼系缓释抑菌剂的继续研究及工业化应用提供了参考依据。

关键词: 硼, 缓释, 抑菌作用

Abstract:

High temperature melting method has been used to synthesize B₂O₃-SiO₂-Na₂O controlled-release antibacterial agent on account of the functions that borate can inhibit the growth of bacteria and algae. The new inhibitor, a solid agent with controlled-release capability in water, is different from those liquid and gaseous antibacterial agents. In this study, the physicochemical properties of the inhibitor were characterized by XRD, SEM and FTIR. The concentrations of boron in solution were determined by using ICP-AES to determine the release capability of the inhibitor. Moreover the experiments of inhibitory circle and inhibitory rate were carried out to evaluate the antibacterial effects of the inhibitor on Escherichia Coli and Staphylococcus Aureus. The results indicate that the inhibitors present amorphous structure, with the content of [BO₃] and [BO₄] groups in the internal structure being affected by the amounts and the ratio of B₂O₃ and SiO₂. The agent surface is denser while the release rate decreases with the decrease of B₂O₃ amount. Furthermore, the antibacterial effect enhances with the increase of boron concentration. S. Aureus is more sensitive to the inhibitor than E. Coli. The inhibitor containing 55wt% of B₂O₃gets the best antibacterial effect , which exhibits better controlled-release than the others. The present results provide a reference for further research and industrial application of the antibacterial agent.

Key words: boron, controlled-release, antibacterial effect

中图分类号:

TQ174

史蕊蕊, 李萌, 裴元生. B₂O₃-SiO₂-Na₂O缓释抑菌剂的合成及性能表征[J]. 无机材料学报, 2017, 32(5): 529-534.

SHI Rui-Rui, LI Meng, PEI Yuan-Sheng. Synthesis and Characterizations of B₂O₃-SiO₂-Na₂O Controlled-release Antibacterial Agent[J]. Journal of Inorganic Materials, 2017, 32(5): 529-534.

图/表 8

表1 B2O3-SiO2-Na2O抑菌剂各组分的含量

Table 1 Composition of the B2O3-SiO2-Na2O inhibitor

Inhibitor	B₂O₃/wt%	Na₂O/wt%	SiO₂/wt%
1	70	12.4	17.6
2	65	11.5	23.5
3	60	10.6	29.4
4	55	9.8	35.2

图1 不同抑菌剂的XRD图谱

Fig. 1 XRD patterns of different inhibitors (a) Inhibitor-1; (b) Inhibitor-2; (c) Inhibitor-3; (d) Inhibitor-4

图2 不同抑菌剂的SEM照片

Fig. 2 SEM images for different inhibitors (a) Inhibitor-1; (b) Inhibitor-2; (c) Inhibitor-3; (d) Inhibitor-4

图3 不同抑菌剂的红外光谱图

Fig. 3 FTIR spectra of different inhibitors (a) Inhibitor-1; (b) Inhibitor-2; (c) Inhibitor-3; (d) Inhibitor-4

表2 硼硅酸盐材料中各基团的红外吸收光谱特征振动

Table 2 Characteristic vibration reflected by infrared absorption spectrum of each group in borosilicate materials

Wavenumber/cm^-1	Corresponding characteristic vibration ascription
3423-3426	H-O stretching vibration peak
1413-1424	[BO₃] anti symmetrical stretching vibration peak
1071-1076	[BO₄] anti symmetrical stretching vibration peak
923-930	[BO₄] anti symmetrical stretching vibration peak
686-696	[BO₃] flexural vibration peak
451-466	Si-O-Si flexural vibration peak

图4 不同抑菌剂在常温下缓释出硼的量(mg/mL)

Fig. 4 Concentration of controlled-releasing boron of different inhibitors at room temperature (mg/mL)

表3 抑菌剂对大肠杆菌和金黄色葡萄球菌的抑菌圈/mm

Table 3 Inhibitory circle of E. Coli and S. Aureus against inhibitor/mm

Inhibitor	Boron /(mg·mL^-1)		E. coli			S. aureus
Inhibitor	Boron /(mg·mL^-1)	0.5	1	1.5	2	0.5	1	1.5	2
1	Diameter	<10	10±0.5	12±0.5	15±0.5	11±0.5	17±0.5	19±0.5	30±0.5
1	Sensitivity	–	+	+	+	+	++	++	+++
2	Diameter	<10	10±0.5	11±0.5	17±0.5	13±0.5	17±0.5	20±0.5	23±0.5
2	Sensitivity	–	+	+	++	+	++	++	+++
3	Diameter	<10	11±0.5	13±0.5	18±0.5	14±0.5	17±0.5	19±0.5	22±0.5
3	Sensitivity	–	+	+	++	+	++	++	+++
4	Diameter	<10	14±0.5	14±0.5	16±0.5	12±0.5	16±0.5	20±0.5	30±0.5
4	Sensitivity	–	+	+	++	+	++	+++	+++

图5 不同抑菌剂对大肠杆菌和金黄色葡萄球菌的抑菌率

Fig. 5 Inhibitory rate of E. Coli and S.Aureus against different inhibitors

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B₂O₃-SiO₂-Na₂O缓释抑菌剂的合成及性能表征

Synthesis and Characterizations of B₂O₃-SiO₂-Na₂O Controlled-release Antibacterial Agent

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