In vitro Antibacterial and Osteogenic Properties of Manganese Doped Nano Hydroxyapatite

doi:10.15541/jim20230438

Abstract

Abstract:

Hydroxyapatite (HAP), as a common bone repair material, still faces the risk of bacterial infection in the treatment of infectious bone defects, whose limited osteogenic properties also hinders its further application. This study used a coprecipitation method to prepare the manganese doped hydroxyapatite nanorod (MnHAP), which exhibited excellent cell biocompatibility, high antibacterial efficiency and osteogenic properties. Antibacterial experiments showed that the inhibition rates of MnHAP10(n(Mn)/n(Ca+Mn)=10%)) against Escherichia coli and Staphylococcus aureus can reach 77.85% and 75.92%, respectively. Moreover, the antibacterial efficiency of MnHAP10 against Escherichia coli can be further enhanced (97.63%) under 808 nm near-infrared light irradiation. Cell proliferation and related osteogenic gene experiments display that MnHAP is beneficial for the proliferation and differentiation of osteoblasts, which improves protein adsorption capacity, stimulates osteogenic activity, and promotes the expression of related osteogenic genes, demonstrating its good biocompatibility. Therefore, MnHAP nanorods are expected to provide a new approach in the treatment of infectious bone defects.

Key words: hydroxyapatite, manganese, antibacterial, infectious bone defect, photothermal

CLC Number:

TQ174

LI Chengyu, DING Ziyou, HAN Yingchao. In vitro Antibacterial and Osteogenic Properties of Manganese Doped Nano Hydroxyapatite[J]. Journal of Inorganic Materials, 2024, 39(3): 313-320.

Figures/Tables 6

Fig. 1 Morphologies and structures of MnHAP (a) XRD patterns of MnHAP and HAP; (b-f) SEM images of HAP (b), MnHAP1 (c), MnHAP5 (d), MnHAP10 (e), and MnHAP20 (f); (g-k) TEM images of HAP (g), MnHAP1 (h), MnHAP5 (i), MnHAP10 (j) and MnHAP20 (k); (l-p) Selected area diffraction pattern of HAP (l), MnHAP1 (m), MnHAP5 (n), MnHAP10 (o), and MnHAP20 (p)

Table 1 Lattice parameters, volume, and crystallinity of MnHAP and HAP

Sample	a-axis/Å	c-axis/Å	Volume/Å³	Crytallinity/%
HAP	9.41129	6.88743	528.31	61.77
MnHAP1	9.41083	6.88255	528.78	62.92
MnHAP5	9.40699	6.88027	527.74	61.72
MnHAP10	9.40415	6.87068	527.4	55.98
MnHAP20	9.40065	6.86074	527.11	50.36

Fig. 2 Manganese ion release curves of MnHAP

Fig. 3 Mn element valence in MnHAP10 (a), photoabsorption of MnHAP (b), photothermal conversion efficiency of MnHAP10 (c), pure antibacterial effect MnHAP (d-e) and photothermal antibacterial MnHAP10 (f)

Fig. 4 Specific surface area and pore structure (a), Zeta potential (b) and protein adsorption amount (c) of MnHAP

Fig. 5 Cell compatibility (a), osteogenic marker (ALP/ARS) (b), and gene expression of protein marker and osteogenesis-related genes (c-h) of MnHAP

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