Research on Preparation and Biological Properties of Dense Hydroxyapatite Spheres

doi:10.3724/SP.J.1077.2013.12038

Abstract

Abstract:

By using carboxy methyl chitosan (CMCS) and gelatin (Gel) as adhesive, the nano-HA slurry which was fabricated via a precipitation process as the raw material, dense HA spheres with a uniform size distribution and good geometry were prepared successfully by means of Sol-Gel method. Dense HA spheres with a porosity of 4.7%±0.6% have a higher compressive strength (8.9±0.4) MPa than that of porous HA spheres (7.9±0.2) MPa with a porosity of 16.4%±0.5% (particles with diameter of 0.5 mm). The results of biomimetic mineralization and cell culture show that dense HA spheres have an excellent biological property.

Key words: hydroxyapatite, Sol-Gel method, high strength, density, biological property

CLC Number:

R318
TQ174

LUO Hui-Tao, ZHI Wei, LU Xiong, LI Jin-Yu, FENG Bo, WENG Jie. Research on Preparation and Biological Properties of Dense Hydroxyapatite Spheres[J]. Journal of Inorganic Materials, 2013, 28(1): 40-44.

Figures/Tables 4

Fig. 1 (a) TEM image of initial HA slurry, (b) XRD patterns of initial HA and as-sintered HA spheres, (c) Optical picture of dense HA spheres, (d) SEM image of dense HA spheres and (e) Sintering curve of HA spheres

Fig. 2 Surface SEM images of three types of HA spheres

Table 1 Properties of spheres with different HA contents

HA	Porosity/%	Shrinkage/%	Compressive strength/MPa
10wt%	16.4±0.5	58.7±0.7	7.9±0.2
20wt%	8.3±0.4	57.9±0.6	8.6±0.2
25wt%	4.7±0.6	57.4±0.9	8.9±0.4

Fig. 3 SEM images of the surface of dense spheres (a) mineralized for 3 d, (b) mineralized for 5 d and (c) cultured for 3 d

References 22

[1]	Zhao J, Duan K, Zhang J W, et al. Preparation of highly interconnected porous hydroxyapatite scaffolds by chitin gel-casting. Mater. Sci. Eng. C, 2011, 31(3): 697-701.
[2]	Descamps M, Hornez J C, Leriche A. Manufacture of hydroxyapatite beads for medical applications. J. Eur. Ceram. Soc., 2009, 29(3): 369-375.
[3]	Fathi M H, Hanifi A, Mortazavi V. Preparation and bioactivity evaluation of bone-like hydroxyapatite nanopowder. J. Mater. Process Technol., 2008, 202(1/2/3): 536-542.
[4]	Ruys A J, Wei M, Sorrell C C, et al. Sintering effects on the strength of hydroxyapatite. Biomaterials, 1995, 16(5): 409-415.
[5]	Muralithran G, Ramesh S. The effects of sintering temperature on the properties of hydroxyapatite. Ceram. Int., 2000, 26(2): 221-230.
[6]	Suchanek W, Yoshimura M. Processing and properties of hydroxyapatite-based biomaterials for use as hard tissue replacement implants. J. Mater. Res., 1998, 13(1): 94-117.
[7]	Rodríguez-Lorenzo L M, Vallet-Regí M, Ferreira J M F, et al. Hydroxyapatite ceramic bodies with tailored mechanical properties for different applications. J. Biomed. Mater. Res., 2002, 60(1): 159-166.
[8]	Kokubo T, Kim H M, Kawashita M. Novel bioactive materials with different mechanical properties. Biomaterials, 2003, 24(13): 2161-2175.
[9]	Jalota S, Tas A C, Bhaduri S B. Microwave-assisted synthesis of calcium phosphate nanowhiskers. Mater. Res. Soc., 2004, 19(6): 1876-1881.
[10]	Gibson I R, Ke S, Best S M, et al. Effect of powder characteristics on the sinterability of hydroxyapatite powders. J. Mater. Sci.: Mater. Med., 2001, 12(2): 163-171.
[11]	Veljković Dj, Jokic B, Jankovic-Castvan I, et al. Sintering behaviour of nanosized HAp powder. Key Eng. Mater., 2007, 330-332(2): 259-262.
[12]	Murray M G S, Wang J, Ponton C B, et al. An improvement in processing of hydroxyapatite ceramics. J. Mater. Sci., 1995, 30(12): 3061-3074.
[13]	Gonda Y, Ioku K, Shibata Y, et al. Stimulatory effect of hydrothermally synthesized biodegradable hydroxyapatite granules on osteogenesis and direct association with osteoclasts. Biomaterials, 2009, 30(26): 4390-4400.
[14]	Fujii S, Okada M, Furuzono T. Hydroxyapatite nanoparticles as stimulus-responsive particulate emulsifiers and building block for porous materials. J. Colloid. Interf. Sci., 2007, 315(1): 287-296.
[15]	Marques da Silva H, Mateescu M, Ponche A, et al. Surface transformation of silicon-doped hydroxyapatite immersed in culture medium under dynamic and static conditions. Colloids Surf. B, 2010, 75(1): 349-355.
[16]	Swain S K, Bhattacharyya S, Sarkar D. Preparation of porous scaffold from hydroxyapatite powders. Mater. Sci. Eng. C, 2011, 31(6): 1240-1244.
[17]	Pual W, Nesamony J, Sharma C P. Delivery of insulin from hydroxyapatite ceramic microspheres: preliminary in vivo studies. J. Biomed. Mater. Res., 2002, 61(4): 660-662.
[18]	Sun R X, Li M S, Lu Y P, et al. Dissolution behavior of hollow hydroxyapatite microspheres immersed in deionized water. Mater. Res. Bull., 2006, 41(6): 1138-1145.
[19]	Teng S H, Chen L J, Guo Y C, et al. Formation of nano- hydroxyapatite in gelatin droplets and the resulting porous composite microspheres. J. Inorg. Biochem., 2007, 101(4): 686-691.
[20]	Wang Q, Huang W H, Wang D P, et al. Preparation of hollow hydroxyapatite microspheres. J. Mater. Sci.: Mater. Med., 2006, 17(7): 641-646.
[21]	Wang J W, Shaw L L. Morphology-enhanced low-temperature sintering of nanocrystalline hydroxyapatete. Adv. Mater., 2007, 19(17): 2364-2369.
[22]	Chu C L, Zhu J C, Yin Z D, et al. Sintering behaviours and mechanical properties of dense hydroxyapatite (HA) bioceramics. J. Funct. Mater., 1999, 30(6): 606-609.