3D打印制备镁黄长石生物陶瓷骨组织工程支架及其性能

doi:10.15541/jim20220635

无机材料学报 ›› 2023, Vol. 38 ›› Issue (7): 763-770.DOI: 10.15541/jim20220635 CSTR: 32189.14.10.15541/jim20220635

所属专题：【生物材料】骨骼与齿类组织修复(202409)；【制备方法】3D打印(202409)

3D打印制备镁黄长石生物陶瓷骨组织工程支架及其性能

施哲¹^,²(), 刘伟业²^,³, 翟东², 谢建军¹(), 朱钰方²()

1.上海大学材料科学与工程学院, 上海 200444
2.中国科学院上海硅酸盐研究所, 上海 200050
3.上海理工大学材料与化学学院, 上海 200093

收稿日期:2022-10-27 修回日期:2022-11-23 出版日期:2023-12-30 网络出版日期:2022-12-28
通讯作者: 谢建军, 副教授. E-mail: xiejianjun@shu.edu.cn;
朱钰方, 研究员. E-mail: zjf2412@163.com
作者简介:施哲(1998-), 男, 硕士研究生. E-mail: zheshi1998@163.com
基金资助:
国家自然科学基金面上项目(51872185)

Akermanite Scaffolds for Bone Tissue Engineering: 3D Printing Using Polymer Precursor and Scaffold Properties

SHI Zhe¹^,²(), LIU Weiye²^,³, ZHAI Dong², XIE Jianjun¹(), ZHU Yufang²()

1. School of Materials Science and Engineering, Shanghai University, Shanghai 200444, China
2. Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China
3. School of Materials and Chemistry, University of Shanghai for Science and Technology, Shanghai 200093, China

Received:2022-10-27 Revised:2022-11-23 Published:2023-12-30 Online:2022-12-28
Contact: XIE Jianjun, associate professor. E-mail: xiejianjun@shu.edu.cn;
ZHU Yufang, professor. E-mail: zjf2412@163.com
About author:SHI Zhe (1998-), male, Master candidate. E-mail: zheshi1998@163.com
Supported by:
National Natural Science Foundation of China(51872185)

摘要/Abstract

摘要：

具备良好成骨性能和降解速率的生物陶瓷骨组织工程支架在骨修复领域极具应用潜力。镁黄长石(Ca₂MgSi₂O₇)因其具有良好的力学性能、生物降解能力以及促成骨性能而备受关注。本研究以硅树脂为聚合物前驱体、碳酸钙与氧化镁为活性填料制备打印浆料, 采用挤出式3D打印技术在室温条件下制备支架素坯, 并在惰性气氛下高温烧结制备了镁黄长石生物陶瓷支架, 并对比研究了镁黄长石支架与斜硅钙石(Ca₂SiO₄)、镁橄榄石(Mg₂SiO₄)支架在结构、抗压强度、体外降解能力以及体外生物学性能等方面的差异。结果表明: 镁黄长石支架与斜硅钙石、镁橄榄石支架具有相似的三维多孔结构, 抗压强度、降解速率介于镁橄榄石和斜硅钙石之间, 但促进骨髓间充质干细胞的成骨基因表达能力显著强于镁橄榄石和斜硅钙石支架。本研究证实采用3D打印制备的镁黄长石支架有望作为骨组织工程较理想的支架。

关键词: 聚合物前驱体, 3D打印, 生物陶瓷

Abstract:

Bioceramic scaffolds with excellent osteogenesis ability and degradation rate exhibit great potential in bone tissue engineering. Akermanite (Ca₂MgSi₂O₇) has attracted much attention due to its good mechanical property, biodegradability and enhanced bone repair ability. Here, akermanite (Ca₂MgSi₂O₇) scaffolds were fabricated by an extrusion-type 3D printing at room temperature and sintering under an inert atmosphere using printing slurry composed of a silicon resin as polymer precursor, and CaCO₃ and MgO as active fillers. Furthermore, the differences in structure, compressive strength, in vitro degradation, and biological properties among akermanite, larnite (Ca₂SiO₄) and forsterite (Mg₂SiO₄) scaffolds were investigated. The results showed that the akermanite scaffold is similar to those of larnite and forsterite in 3D porous structure, and its compressive strength and degradation rate were between those of the larnite and forsterite scaffolds, but it showed a greater ability to stimulate osteogenic gene expression of rabbit bone marrow mesenchymal stem cells (rBMSCs) than both larnite and forsterite scaffolds. Hence, such 3D printed akermanite scaffold possesses great potential for bone tissue engineering.

Key words: polymer precursor, 3D printing, bioceramics

中图分类号:

TQ174

施哲, 刘伟业, 翟东, 谢建军, 朱钰方. 3D打印制备镁黄长石生物陶瓷骨组织工程支架及其性能[J]. 无机材料学报, 2023, 38(7): 763-770.

SHI Zhe, LIU Weiye, ZHAI Dong, XIE Jianjun, ZHU Yufang. Akermanite Scaffolds for Bone Tissue Engineering: 3D Printing Using Polymer Precursor and Scaffold Properties[J]. Journal of Inorganic Materials, 2023, 38(7): 763-770.

图/表 7

图1 1400 ℃烧结不同钙镁硅比例支架的XRD图谱

Fig. 1 XRD patterns of the scaffolds with different Ca : Mg : Si ratio sintered at 1400 ℃

图2 (a1, a2)C2S、(b1, b2)AKT和(c1, c2)M2S 生物陶瓷支架的表面SEM照片

Fig. 2 SEM images of the surfaces of bioceramic scaffolds (a1, a2) C2S, (b1, b2) AKT and (c1, c2) M2S

图3 (a1~a3)C2S、(b1~b3)AKT和(c1~c3)M2S生物陶瓷支架的断面SEM照片

Fig. 3 SEM images of the fracture surfaces of bioceramic scaffolds (a1-a3) C2S, (b1-b3) AKT and (c1-c3) M2S

图4 C2S、AKT和M2S生物陶瓷支架的(a)孔隙率和(b)抗压强度

Fig. 4 (a) Porosities and (b) compressive strengths of C2S, AKT and M2S bioceramic scaffolds *P < 0.05; **P < 0.01; ***P < 0.001

图5 (a) C2S、AKT和M2S支架在Tris-HCl溶液中浸泡21 d的降解剩余质量和 (b) 溶液的pH变化曲线

Fig. 5 (a) Residual mass curves and (b) pH changes of C2S, AKT and M2S bioceramic scaffolds after soaking in Tris-HCl buffer for 21 d

图6 rBMSCs在C2S、AKT和M2S支架上(a)培养1、3和7 d的细胞增殖情况和(b)培养7 d的碱性磷酸酶(ALP)相对活性表达

Fig. 6 (a) Cell proliferation of culturing for 1, 3 and 7 d and (b) relative activity of alkaline phosphatase of rBMSCs culturing for 7 d on C2S, AKT and M2S bioceramic scaffolds, respectively *P < 0.05; **P < 0.01; ***P < 0.001

图7 rBMSCs在C2S、AKT和M2S支架上培养7 d的相关成骨基因的表达

Fig. 7 Related osteogenic gene expression of rBMSCs on C2S, AKT and M2S scaffolds after culturing for 7 d (a) OCN; (b) Runx-2; (c) OPN; (d) BSP; (e) Col-Ⅰ *P < 0.05; **P < 0.01; ***P < 0.001

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3D打印制备镁黄长石生物陶瓷骨组织工程支架及其性能

Akermanite Scaffolds for Bone Tissue Engineering: 3D Printing Using Polymer Precursor and Scaffold Properties

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