聚多巴胺螯合钙离子对钛表面的修饰及修饰后的细胞相容性

doi:10.15541/jim20150097

无机材料学报 ›› 2015, Vol. 30 ›› Issue (10): 1075-1080.DOI: 10.15541/jim20150097 CSTR: 32189.14.10.15541/jim20150097

聚多巴胺螯合钙离子对钛表面的修饰及修饰后的细胞相容性

谭帼馨¹, 欧阳孔友¹, 周蕾², 刘燕¹, 张兰³, 宁成云²

(1. 广东工业大学轻工化工学院, 广州510006; 2. 华南理工大学材料科学与工程学院, 广州510641; 3. 西安交通大学金属材料国家重点实验室, 西安710049)

收稿日期:2015-02-11 修回日期:2015-04-09 出版日期:2015-10-20 网络出版日期:2015-09-30
作者简介:谭帼馨(1971–), 女, 副教授. E-mail: tanguoxin@126.com
基金资助:
国家973计划项目(2012CB619100);国家自然科学基金(51372087);广东省科技计划项目(2014A010105048);广东省自然科学基金(2015A030313493);西安交通大学金属材料强度国家重点实验室开放研究项目(20141607)

Titanium Modification by Calcium Ion Chelated Polydopamine and Its Cytocompatibility

TAN Guo-Xin¹, OUYANG Kong-You¹, ZHOU Lei², LIU Yan¹, ZHANG Lan³, NING Cheng-Yun²

(1. School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, China; 2. College of Materials Science and Technology, South China University of Technology, Guangzhou 510641, China; 3. State Key Laboratory for Mechanical Behavior of Materials, Xi’an Jiaotong University, Xi’an 710049, China)

Received:2015-02-11 Revised:2015-04-09 Published:2015-10-20 Online:2015-09-30
About author:TAN Guo-Xin. E-mail: tanguoxin@126.com
Supported by:
National Basic Research Program of China(2012CB619100);National Natural Science Foundation of China (51372087);Science and Technology Planing Project of Guangdong Province(2014A010105048);Natural Science Foundation of Guangdong Province(2015A030313493);Supported by State Key Laboratory for Mechanical Behavior of Materials(20141607)

摘要/Abstract

摘要：

通过聚多巴胺自组装在钛表面构建钙离子螯合平台, 探讨聚多巴胺的酚羟基(-C-OH)和醌基(-C=O)螯合钙离子机理, 并研究其生物学性能。利用X射线光电子能谱分析聚多巴胺螯合钙离子化学基团的变化, 结果显示: 聚多巴胺螯合钙离子后, -C-OH峰面积从73.8%降至37.3%, -C=O从26.2%升至62.7%, 证明聚多巴胺的酚羟基与钙离子螯合过程中向醌基转化; 场发射扫描电镜、傅立叶红外光谱、X射线衍射仪和透射电镜分析表明聚多巴胺和钙离子的协同作用促进羟基磷灰石(HA)前驱体成核, 诱导HA的形成; 采用小鼠成骨细胞(MC3T3-E1)粘附和活性实验进行细胞相容性评价, 结果表明钛表面通过聚多巴胺螯合低浓度的钙离子有利于成骨细胞粘附, 且具有良好的细胞相容性。

关键词: 聚多巴胺, 螯合机理, 钙离子, 生物活性, 细胞相容性

Abstract:

Calcium ions chelation platform was successfully obtained on a titanium surface by modification with polydopamine. The chelation mechanism of polydopamine with Ca²⁺ was studied. The in vitro bioactivity and cytocompatibility of Ca²⁺ chelation platform was assessed by incubation in simulated body fluids (SBF) and cultural osteoblast cells (MC3T3-E1), respectively. The results showed that the functional groups of polydopamine chelated Ca²⁺ were changed from phenolic hydroxyl to benzoquinone. The titanium surface modified by Ca²⁺-chelated polydopamine could induce nucleation and growth of hydroxyapatite in SBF. Meanwhile, Cell attachment and viability assay demonstrated that low concentration of Ca²⁺ chelation possessed good cytocompatibility.

Key words: polydopamine, chelation mechanism, calcium ion, bioactivity, cytocompatibility

中图分类号:

TB331

谭帼馨, 欧阳孔友, 周蕾, 刘燕, 张兰, 宁成云. 聚多巴胺螯合钙离子对钛表面的修饰及修饰后的细胞相容性[J]. 无机材料学报, 2015, 30(10): 1075-1080.

TAN Guo-Xin, OUYANG Kong-You, ZHOU Lei, LIU Yan, ZHANG Lan, NING Cheng-Yun. Titanium Modification by Calcium Ion Chelated Polydopamine and Its Cytocompatibility[J]. Journal of Inorganic Materials, 2015, 30(10): 1075-1080.

图/表 7

图1 样品的XPS全谱图

Fig. 1 XPS full spectra of samples

表1 样品表面XPS各元素含量分析结果

Table1 Chemical compositions of the surface layers of samples as analyzed by XPS

Samples	Element /at%					Ratio
Samples	C	O	N	Ti	Ca	N/C
Ti-OH	37.43	44.95	0.86	16.76	-	0.023
Ti-PDA	68.56	22.89	8.30	0.25	-	0.121
Ti-PDA-0.1M Ca²⁺	65.19	26.37	7.34	0.51	0.50	0.113
Ti-PDA-0.5M Ca²⁺	62.71	28.33	6.40	-	2.56	0.102

图2 聚多巴胺钛表面螯合钙离子高分辨XPS分析

Fig. 2 Surface analysis by XPS showing that polydopamine binds to calcium ion, chelating Ca2+ in CaCl2 solution

图3 聚多巴胺钛表面螯合钙离子37℃下浸泡于SBF中0、3和7 d时所形成的羟基磷灰石SEM照片及其形成机理示意图

Fig. 3 FE-SEM images(a-d) and mineralization mechanism diagram (e) of hydroxyapatite formation on polydopamine surface preloaded with Ca2+ in SBF at 37℃ for 0, 3 and 7 d

图4 样品矿化7 d的FT-IR分析

Fig. 4 FT-IR of the mineralized hydroxyapatite at 37℃ after 7 d being soaked in SBF

图5 Ti-PDA-0.5M Ca2+表面矿化7 d羟基磷灰石形貌和晶型结构分析

Fig. 5 Hydroxyapatite structural analysis of mineralization on Ti-PDA-0.5M Ca2+ after 7 d being soaked in SBF. (a) SEM, (b) XRD, (c) TEM and (d) SAED

图6 样品表面MC3T3-E1细胞在粘附24 h的(a)SEM照片及其(b)细胞荧光染色图(绿为活细胞, 红为死细胞)

Fig. 6 (a) SEM images of cells morphology and (b) fluorescence images of live/dead staining of osteoblasts (MC3T3-E1) after culturing for 24 h (the live cells are in green and dead cells in red)

参考文献 17

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聚多巴胺螯合钙离子对钛表面的修饰及修饰后的细胞相容性

Titanium Modification by Calcium Ion Chelated Polydopamine and Its Cytocompatibility

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