近红外光响应的双相抗菌介孔生物活性玻璃复合支架的制备及抗菌性能研究

doi:10.15541/jim20250030

摘要/Abstract

摘要： 骨植入材料易感染且具有高发性、迟发性和复发性。然而,目前常见的抗菌手段通常缺乏选择性,容易在杀菌的同时对正常组织和细胞造成损害。基于此,本研究以介孔生物活性玻璃(MBG)为原料,结合S-NO键近红外光热响应释放NO自由基及Cu²⁺抗菌性能,采用氨基化偶联S-亚硝基硫醇(RSNO)制备了MBG-RSNO粉体以供给NO·,并同时由多巴胺聚合及Cu²⁺螯合合成了PMBG@Cu粉体。将上述两种粉体材料经3D打印得到PMBG@Cu/MBG-RSNO复合抗菌支架。该支架具有近红外光热响应特性,在808 nm激光脉冲式辐照条件下,支架持续释放的NO·含量高达113.71 μg/100 mg MBG-RSNO,且光热作用将温度升高至约40 ℃,有利于实现高效灭菌。进一步使用金黄色葡萄球菌(S. aureus)和大肠杆菌(E. coli)对复合支架在有无外部近红外刺激条件下的抗菌效果进行了评估。实验结果显示,PMBG@Cu/MBG-RSNO复合支架对两种菌种的抗菌效率均达到了99.9%。综上所述,PMBG@Cu/MBG-RSNO复合支架为解决骨植入材料的感染问题并实现骨缺损修复提供了一种潜在的解决方案。

关键词: 介孔生物活性玻璃, 抗菌, 一氧化氮, 光热效应

Abstract: Bone implant-related infections are characterized by high-risk of delayed incidence or recurrence. Current antibacterial strategies often lack selectivity, leading to collateral damage to normal tissues and cells during bacterial eradication. To address this, mesoporous bioactive glass (MBG) was used as a base material in this study. By leveraging the near-infrared (NIR) photothermal response of S-NO bonds to release NO radicals and the antibacterial properties of Cu²⁺, MBG-RSNO powder was synthesized through amino-functionalized conjugation of S-nitrosothiols (RSNO) for NO· delivery, while PMBG@Cu powder was prepared via dopamine polymerization and Cu²⁺chelation. These two powder materials were further processed through 3D printing to fabricate a PMBG@Cu/MBG-RSNO composite antibacterial scaffold. This scaffold demonstrated a strong NIR photothermal response, with pulsed 808 nm laser irradiation enabling the sustained release of NO· up to 113.71 μg per 100 mg MBG-RSNO and increasing the temperature to approximately 40 °C, facilitating efficient bacterial elimination. Antibacterial performance of the scaffold was evaluated using Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) as representative Gram-positive and Gram-negative bacteria, respectively, under conditions with and without external NIR stimulation. The results revealed that the PMBG@Cu/MBG-RSNO composite scaffold achieved an antibacterial efficiency of 99.9% against both bacterial strains. In summary, the PMBG@Cu/MBG-RSNO composite scaffold offers a promising solution to address infection challenges in bone implant materials and supports bone defect repair.

Key words: mesoporous bioactive glass, antibacterial, nitric oxide, photothermal effect

中图分类号:

TQ171

张博, 付一敏, 陈政, 石澳, 朱敏. 近红外光响应的双相抗菌介孔生物活性玻璃复合支架的制备及抗菌性能研究[J]. 无机材料学报, DOI: 10.15541/jim20250030.

ZHANG Bo, FU Yimin, CHEN Zheng, SHI Ao, ZHU Min. Near-infrared Responsive Biphasic Antibacterial Mesoporous Bioactive Glass Composite Scaffolds: Preparation and Antibacterial Performance[J]. Journal of Inorganic Materials, DOI: 10.15541/jim20250030.

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