不同模拟体液对硼硅酸盐生物活性玻璃基骨水泥矿化性能的影响

doi:10.15541/jim20200484

无机材料学报 ›› 2021, Vol. 36 ›› Issue (7): 745-752.DOI: 10.15541/jim20200484 CSTR: 32189.14.10.15541/jim20200484

不同模拟体液对硼硅酸盐生物活性玻璃基骨水泥矿化性能的影响

林子扬¹(), 常宇辰², 吴章凡¹, 包荣³, 林文庆³, 王德平¹()

1.同济大学材料科学与工程学院, 上海 201804
2.伽蓝(集团)股份有限公司, 上海 200030
3.上海三悠树脂有限公司, 上海 201818

收稿日期:2020-08-20 修回日期:2020-09-18 出版日期:2021-07-20 网络出版日期:2020-10-10
通讯作者: 王德平, 教授. E-mail:wdpshk@tongji.edu.cn
作者简介:林子扬(1996-), 男, 硕士研究生. E-mail:lzyyymx@163.com
基金资助:
国家重点研发计划(2018YFC1106300);上海三悠树脂有限公司(05002450376)

Different Simulated Body Fluid on Mineralization of Borosilicate Bioactive Glass-based Bone Cement

LIN Ziyang¹(), CHANG Yuchen², WU Zhangfan¹, BAO Rong³, LIN Wenqing³, WANG Deping¹()

1. School of Materials Science and Engineering, Tongji University, Shanghai 201804, China
2. JALA (Group) Co., Ltd, Shanghai 200030, China
3. Shanghai Sanyu Resin Co., Ltd, Shanghai 201818, China

Received:2020-08-20 Revised:2020-09-18 Published:2021-07-20 Online:2020-10-10
Contact: WANG Deping, professor. E-mail:wdpshk@tongji.edu.cn
About author:LIN Ziyang(1996-), male, Master candidate. E-mail:lzyyymx@163.com
Supported by:
National Key Research and Development Projects(2018YFC1106300);Shanghai Sanyu Resin Co., Ltd(05002450376)

摘要/Abstract

摘要：

硼硅酸盐生物活性玻璃基(Borosilicate bioactive glass-based, BBG)骨水泥由于其优异的生物活性和生物降解性, 在治疗骨质疏松性骨折以及骨肿瘤、骨创伤、骨髓炎等疾病方面具有重要的应用前景, 受到人们的广泛关注。为进一步了解氨基酸对其植入生物体内后的矿化影响, 本研究在常规的SBF溶液中添加了不同种类及浓度的氨基酸物质, 重点研究对植入体表面形貌的影响。同时为在矿化过程中同步形成白磷钙矿(Whitlockite, WH)和羟基磷灰石(hydroxyapatite, HA), 调整了SBF溶液的温度以及酸碱度和Mg²⁺浓度, 研究了不同SBF溶液中BBG骨水泥表面形成的矿化产物。研究结果表明, 不同的氨基酸及浓度的变化对矿化产物的影响有较大差异, 天冬氨酸和赖氨酸的浓度变化影响矿物的长径比, 而甘氨酸对矿物形貌的影响较小。将硼硅酸盐生物活性玻璃压片放置在70 ℃下的高Mg²⁺浓度的酸性(pH=3.5)SBF溶液中浸泡一定时间后, 能够获得HA/WH的复相矿物。

关键词: 模拟体液, 氨基酸, 硼硅酸盐生物活性玻璃基骨水泥, 白磷钙矿, 羟基磷灰石

Abstract:

Borosilicate bioactive glass-based (BBG) bone cement has important application prospects in the treatment of osteoporotic fractures, bone tumors, bone trauma, osteomyelitis, and other diseases due to its excellent bioactivity and biodegradability. To further understand the factors affecting its mineralization ability after implantation into human body, three kinds of amino acid were added into the conventional simulated body fluids (SBF) solution. Furthermore, to get whitlockite (WH) and hydroxyapatite (HA) simultaneously during the mineralization process, the environmental temperature, pH and Mg²⁺ concentration of SBF were adjusted. The mineralized products of BBG bone cement after being immersed in different SBF solutions were characterized. It is found that the effects of amino acids on mineralization products are quite different, of which aspartic acid and lysine affect the aspect ratio of the mineral, but glycine does not affect the morphology of the mineral. After the borosilicate bioactive glass tablet being immersed in an acidic (pH 3.5) SBF solution with a high Mg²⁺ content at 70 ℃ for a certain period, HA/WH complex mineral can be obtained.

Key words: simulated boby fluid, amino acid, borosilicate bioactive glass-based bone cement, whitlickite, hydroxyapatite

中图分类号:

TQ171

林子扬, 常宇辰, 吴章凡, 包荣, 林文庆, 王德平. 不同模拟体液对硼硅酸盐生物活性玻璃基骨水泥矿化性能的影响[J]. 无机材料学报, 2021, 36(7): 745-752.

LIN Ziyang, CHANG Yuchen, WU Zhangfan, BAO Rong, LIN Wenqing, WANG Deping. Different Simulated Body Fluid on Mineralization of Borosilicate Bioactive Glass-based Bone Cement[J]. Journal of Inorganic Materials, 2021, 36(7): 745-752.

图/表 11

表1 不同SBF溶液的组成/(mmol·L-1)

Table 1 Composition of different SBFs/(mmol·L-1)

Ion	Na⁺	K⁺	Mg²⁺	Ca²⁺	Cl^-	HCO- 3	HPO2- 4	SO2- 4	pH
Blood plasma	142.0	5.0	1.5	2.5	103.0	27.0	1.0	0.5	7.2-7.4
SBF	142.0	5.0	1.5	2.5	147.8	4.2	1.0	0.5	7.4
1.5SBF	213.0	7.5	2.25	3.75	221.7	6.3	1.5	0.75	7.4
2Mg-1.5SBF	213.0	7.5	4.5	3.75	226.2	6.3	1.5	0.75	3.5

图1 浸泡前骨水泥的(a)表面形貌及(b )XRD图谱

Fig. 1 (a) Surface morphology and (b) XRD pattern of BBG bone cement before being soaked

图2 (a)天冬氨酸、(b)甘氨酸、(c)赖氨酸浓度为0.1 mol/L时和(d)对照组骨水泥矿化(1) 5、(2) 7和(3) 14 d后表面形貌

Fig. 2 Surface morphologies of BBG bone cements after being soaked in 1.5SBF containing 0.1 mol/L (a) aspartic acid, (b) glycine, (c) lysine, and (d) control group without amino acid for (1) 5, (2) 7 and (3) 14 d

图3 骨水泥在含有0.1 mol/L氨基酸的1.5SBF中浸泡(a) 5和(b)14 d后产物的XRD图谱

Fig. 3 XRD patterns of the products produced by soaking bone cements in 1.5SBF containing 0.1 mol/L amino acid for (a) 5 and (b) 14 d

图4 骨水泥在含有(a) 0.1和(b) 0.2 mol/L 氨基酸的1.5SBF中矿化过程的pH变化

Fig. 4 pH Changes of 1.5SBF containing (a) 0.1 and (b) 0.2 mol/L amino acid during the mineralization of bone cements

图5 (a)天冬氨酸、(b)甘氨酸、(c)赖氨酸浓度为0.2 mol/L时骨水泥矿化(1) 5、(2) 7和(3) 14 d后表面形貌

Fig. 5 Surface morphologies of BBG bone cements after being soaked in 1.5SBF containing 0.2 mol/L (a) aspartic acid, (b) glycine and (c) lysine for (1) 5, (2)7 and (3) 14 d

图6 骨水泥在不同浓度氨基酸溶液中矿化产物XRD图谱

Fig. 6 XRD patterns of the products produced in 1.5SBF with different amino acids at different concentrations

图7 骨水泥在不同浓度氨基酸溶液中矿化产物(211)和(002)晶面的XRD峰相对强度

Fig. 7 Relative intensities of (211) and (002) peaks in XRD patterns of mineralization products

图8 BBG动态浸泡7 和10 d后产物的XRD图谱以及HA和WH的标准XRD图谱

Fig. 8 XRD patterns of BBG after dynamic soaking for 7 and 10 d against the standard XRD patterns of HA and WH

图9 矿化产物中(a, b) HA和(c, d) WH的(a, c) TEM照片和 (b, d)选区电子衍射花样

Fig. 9 (a, c) TEM images and (b, d) selected area electron diffraction patterns of mineralized (a, b) HA and (c, d) WH

图10 BBG动态浸泡在2Mg-1.5SBF矿化7 d后产物表面SEM形貌照片

Fig. 10 SEM image of BBG mineralized surface after being immersed in 2Mg-1.5SBF for 7 d

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不同模拟体液对硼硅酸盐生物活性玻璃基骨水泥矿化性能的影响

Different Simulated Body Fluid on Mineralization of Borosilicate Bioactive Glass-based Bone Cement

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