硼硅酸盐生物活性玻璃在直流电场下的体外矿化性能

doi:10.15541/jim20210030

无机材料学报 ›› 2021, Vol. 36 ›› Issue (9): 1006-1012.DOI: 10.15541/jim20210030

所属专题：【生物材料】骨骼与齿类组织修复

• 研究快报 • 上一篇

硼硅酸盐生物活性玻璃在直流电场下的体外矿化性能

朱子旻¹(), 张敏慧¹, 张轩宇¹, 姚爱华¹^,²(), 林健¹^,²(), 王德平¹^,²

1.同济大学材料科学与工程学院, 上海 201804
2.同济大学教育部土木工程先进材料重点实验室, 上海 200092

收稿日期:2021-01-15 修回日期:2021-02-08 出版日期:2021-09-20 网络出版日期:2021-03-12
通讯作者: 姚爱华, 副教授. E-mail: aihyao@126.com; 林健, 教授. E-mail: lin_jian@tongji.edu.cn
作者简介:朱子旻(1996-), 女, 硕士研究生. E-mail: zzm0605@tongji.edu.cn

In Vitro Mineralization Property of Borosilicate Bioactive Glass under DC Electric Field

ZHU Zimin¹(), ZHANG Minhui¹, ZHANG Xuanyu¹, YAO Aihua¹^,²(), LIN Jian¹^,²(), WANG Deping¹^,²

1. School of Materials Science and Engineering, Tongji University, Shanghai 201804, China
2. Key Laboratory of Advanced Civil Engineering Materials, Ministry of Education, Tongji University, Shanghai 200092, China

Received:2021-01-15 Revised:2021-02-08 Published:2021-09-20 Online:2021-03-12
Contact: YAO Aihua, associate professor. E-mail: aihyao@126.com; LIN Jian, professor. E-mail: lin_jian@tongji.edu.cn
About author:ZHU Zimin (1996-), female, Master candidate. E-mail: zzm0605@tongji.edu.cn
Supported by:
National Key Research and Development Projects(2018YFC1106300);National Natural Science Foundation of China(51972232)

摘要/Abstract

摘要：

硼硅酸盐生物活性玻璃具有良好的生物活性和骨传导性, 但大多数生物活性玻璃表现出非线性降解和矿化行为, 矿化性能会随着时间而减缓。电场作为一种外场辅助调节的方法, 能够干预玻璃的离子交换和扩散。本研究利用直流电场干预硼硅酸盐生物活性玻璃的体外矿化, 加快降解较慢阶段中硼硅酸盐生物玻璃的生物活性。将熔融法制备的成分为18SiO₂-6Na₂O-8K₂O-8MgO-22CaO-2P₂O₅-36B₂O₃的硼硅酸盐生物活性玻璃浸泡在SBF生理模拟液中, 施加0~90 mA的电流, 研究直流电场对硼硅酸盐生物玻璃降解及体外矿化性能的影响。研究结果表明, 施加电场不仅可以提高硼硅酸盐生物活性玻璃的降解率和离子释放量, 而且有利于玻璃网络水解和表面羟基化, 加速羟基磷灰石的生成。其中失重率比对照组提高了3%~5%, 硼和钙的离子释放量分别较对照组提高了2.3~2.9倍和1.9~2.3倍。对硼硅酸盐生物活性玻璃表面结构分析得出, 暴露在电场下的样品表面生成了磷灰石层。应用直流电场可以提高生物活性玻璃的降解及体外矿化性能, 为提升骨修复效果提供了一种新思路。

关键词: 硼硅酸盐生物活性玻璃, 直流电场, 矿化性能

Abstract:

Borosilicate bioactive glass has excellent bioactivity and bone conductivity, but most bioactive glasses exhibit nonlinear degradation and mineralization behavior, with mineralization property declining over time. The direct current (DC) electric field, as an outfield-assisted approach to regulation, can interfere in the ion exchange and diffusion of the glass to modify its property. In this study, a DC electric field is used to intervene in vitro mineralization of borosilicate bioactive glass to accelerate the bioactivity in the slower degradation phase. Borosilicate bioactive glass with the composition of 18SiO₂-6Na₂O-8K₂O-8MgO-22CaO-2P₂O₅-36B₂O₃, prepared by the melting method, was immersed in simulated body fluid (SBF). A current in range of 0-90 mA was applied to study the effect of DC electric field on the degradation and in vitro mineralization property of borosilicate bioactive glass. The results show that the application of electric field increases the degradation rate and ion release of borosilicate bioactive glass. Compared to the control group (without electric field), the weight loss rate increased by 3%-5% and the dissolution of B and Ca ions increased by 2.3-2.9 and 1.9-2.3 times, respectively. Meanwhile, the electric field assists glass network hydrolysis and surface hydroxylation, accelerating the generation of hydroxyapatite (HA). Analyzing the surface structure of the borosilicate bioactive glass particles, we found that an apatite layer was formed on the surface of the sample exposed to the electric field. Hence, the application of a DC electric field can improve the degradation and in vitro mineralization property of bioactive glass, providing a new idea for bone repair effect enhancement.

Key words: borosilicate bioactive glass, DC electric field, mineralization property

中图分类号:

TQ171

朱子旻, 张敏慧, 张轩宇, 姚爱华, 林健, 王德平. 硼硅酸盐生物活性玻璃在直流电场下的体外矿化性能[J]. 无机材料学报, 2021, 36(9): 1006-1012.

ZHU Zimin, ZHANG Minhui, ZHANG Xuanyu, YAO Aihua, LIN Jian, WANG Deping. In Vitro Mineralization Property of Borosilicate Bioactive Glass under DC Electric Field[J]. Journal of Inorganic Materials, 2021, 36(9): 1006-1012.

图/表 8

Fig. 1 Scheme of DC electric field

Fig. 2 Weight loss (a) and comparison of cumulative ion concentration (b) of borosilicate bioactive glass immersed in SBF with different currents applied for 3 d at 37 ℃

Fig. 3 XRD patterns of bioactive glass immersed in SBF with an applied electric field for 14 d

Fig. 4 Microscopic morphologies of borosilicate bioactive glass immersed in SBF for 3 d after applying electric field and EDS analysis of the formed HA (a1, a2, a3) Without electric field; (b1, b2, b3) 30 mA; (c1, c2, c3) 60 mA; (d1, d2, d3) 90 mA

Fig. 5 FT-IR spectra of bioactive glass immersed in SBF with an applied electric field

Table 1 Elemental atomic composition of the surface of bioactive glass particles/at%

	Ca	P	Mg	Na	Si	B
0 mA	16.55	8.79	7.98	5.36	26.65	34.67
60 mA	21.10	10.66	5.87	8.69	9.18	44.50
Original glass	16.18	2.94	5.88	8.82	13.24	52.94

Fig. 6 XPS survey spectra of bioactive glass surface

Fig. 7 XPS spectra of bioactive glass surface

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硼硅酸盐生物活性玻璃在直流电场下的体外矿化性能

In Vitro Mineralization Property of Borosilicate Bioactive Glass under DC Electric Field

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