无机材料学报 ›› 2019, Vol. 34 ›› Issue (11): 1156-1160.DOI: 10.15541/jim20190031 CSTR: 32189.14.10.15541/jim20190031
收稿日期:
2019-01-16
修回日期:
2019-04-18
出版日期:
2019-11-20
网络出版日期:
2019-05-29
作者简介:
王君诚(1993-), 男, 硕士研究生. E-mail: wangjuncheng@whut.edu.cn
基金资助:
WANG Jun-Cheng1,YANG Fei-Fei2,GAO Guan-Bin1(),SUN Tao-Lei1,2(
)
Received:
2019-01-16
Revised:
2019-04-18
Published:
2019-11-20
Online:
2019-05-29
Supported by:
摘要:
近红外荧光特别是近红外Ⅱ区(1000~1700 nm)荧光在生物体内具有高组织渗透率、高时空分辨率、低背景荧光干扰和低光损伤的特点, 因此发展水溶性与生物相容性良好、量子产率高的长波段近红外荧光探针意义重大。本研究制备了不同荧光发射的Ag掺杂HgS量子点(HgAgS量子点)。在不同pH溶液中制备的HgAgS量子点荧光发射峰位于近红外Ⅱ区, 且呈现规律性变化; 随pH的增大, HgAgS量子点荧光发射峰先红移而后蓝移, 发射波长在pH 6时达到最大1110 nm; 原子吸收光谱表明在不同pH溶液中制备的HgAgS量子点, Ag的掺杂量(Ag/Hg比值)呈现出与荧光发射峰相同的规律性变化, 证明通过pH调控Ag的掺杂量从而调谐荧光发射峰的位置。HgAgS量子点的量子产率随pH先增加后降低, 在pH 7时达到最大13.23%(λem=1100 nm)。细胞毒性实验表明Ag的掺杂量对HgAgS量子点的细胞毒性无明显影响, 在1~50 μg/L浓度范围内均无明显细胞毒性。本研究结果不仅为体内进行近红外荧光成像提供了基础研究数据, 而且为荧光纳米探针的设计与制备提出了新的见解。
中图分类号:
王君诚, 杨菲菲, 高冠斌, 孙涛垒. Ag掺杂HgS量子点: 一种pH调谐的近红外Ⅱ区荧光纳米探针[J]. 无机材料学报, 2019, 34(11): 1156-1160.
WANG Jun-Cheng, YANG Fei-Fei, GAO Guan-Bin, SUN Tao-Lei. Ag doped HgS Quantum Dots: a pH-tunable Near-infrared-Ⅱ Fluorescent Nanoprobe[J]. Journal of Inorganic Materials, 2019, 34(11): 1156-1160.
图1 HgAgS量子点的X射线衍射图谱(a), 透射电子显微镜照片(b), 高分辨透射电镜照片(c)和粒径统计分布图(d)
Fig. 1 XRD pattern (a), TEM image (b), HR-TEM image (c), and size distribution (d) of HgAgS QDs
图2 HgAgS量子点的扫描透射电子显微镜高角环形暗场照片(HAADF) (a)和能谱图(b), 及其Ag(蓝色)、Hg(红色)和S(黄色)三种元素混合(c)和对应的单一元素Ag(d)、Hg(e)和S(f)分布图
Fig. 2 High-angle annular dark field (HAADF), STEM image (a) and EDS (b) of HgAgS QDs; Mixed elements mapping of HgAgS QDs (c) with corresponding single element Ag(blue) (d), Hg(red) (e) and S(yellow) (f) elements distribution
图4 激发波长为400 nm时在不同pH下制备的HgAgS量子点的荧光光谱(a)及其Ag/Hg值变化(彩图见网页)
Fig. 4 Fluorescence (a) and Ag/Hg value (b) of HgAgS QDs with different synthetic pH (Ex=400 nm) (colourful edition is available on website)
图5 HgAgS量子点(pH=6.0制备)在不同时间下对INS-1细胞的细胞活性影响(a)和不同pH条件下制备的HgAgS量子点对PC12细胞的活性影响(b)
Fig. 5 Cell viability of HgAgS QDs prepared at pH 6.0 on INS-1 cells for 24 and 48 h (a), and cell viability of HgAgS QDs prepared at pH=3.0, 5.0, 6.0 and 11.0 on PC12 cells (b). Concentrations of HgAgS QDs were 0, 1, 10, 20 and 50 μg/L
图8 图S3 HgS量子点(黑色曲线)和HgAgS量子点(红色曲线)的全谱(a)、Ag3d(b)、Hg4f(c)和S2p(d)的特征谱
Fig. 8 Fig. S3 Survey spectra (a), Ag3d (b), Hg4f (c) and S2p (d) spectra of HgS QDs (black curves) and HgAgS QDs (red curves)
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