微振动应力环境影响羟基磷灰石陶瓷生物活性及力学稳定性的体外评价

doi:10.15541/jim20180240

摘要/Abstract

摘要：

本研究考察微振动应力环境(MVS)下羟基磷灰石(HA)陶瓷的生物活性及力学稳定性, 探讨体内生理应力环境对HA陶瓷骨诱导性的影响。扫描电子显微镜(SEM)和钙离子释放结果均显示, MVS有利于具有较高微孔隙率((29±1.2)%, (26.4±0.3)%)HA材料的钙离子再沉积和类骨磷灰石层形成, 而对于微孔隙率较低((10.6±0.8)%)的HA材料则促进其钙离子释放。蛋白吸附结果显示40 Hz的MVS促进了HA的蛋白吸附, 60 Hz的MVS则显著抑制HA的蛋白吸附行为。抗压试验表明MVS应力环境并未影响HA多孔支架的力学稳定性。研究表明应力环境是影响HA陶瓷生物活性和骨诱导性的重要因素。

关键词: 微振动, 羟基磷灰石, 生物活性, 力学稳定性

Abstract:

This study investigated the bioactivity and mechanical stability of hydroxyapatite (HA) ceramics under micro-vibration stress environment (MVS), and explored influence of physiological stress environment on osteoinductivity of HA ceramics. Results of scanning electron microscopy (SEM) and calcium ion release showed that MVS was beneficial for calcium ion re-deposition and bone-like apatite layer formation in HA with high micro-porosity ((29±1.2)%, (26.4±0.3)%, respectively), while calcium ion release of HA materials with a lower micro-porosity ((10.6±0.8)%) was promoted. Protein adsorption results displayed that 40 Hz MVS promoted while 60 Hz MVS significantly inhibited HA protein adsorption behavior. The compression tests demonstrated that stress environment of MVS did not affect the mechanical stability of the HA scaffolds. Our results suggested that stress environment is an important factor affecting the bioactivity and osteoinductivity of HA ceramics.

Key words: micro-vibration, hydroxyapatite, bioactivity, mechanical properties

中图分类号:

R318
TQ174

吴金结, 李艳, 魏仁初, 汪建新, 屈树新, 翁杰, 智伟. 微振动应力环境影响羟基磷灰石陶瓷生物活性及力学稳定性的体外评价[J]. 无机材料学报, 2019, 34(4): 417-424.

Jin-Jie WU, Yan LI, Ren-Chu WEI, Jian-Xin WANG, Shu-Xin QU, Jie WENG, Wei ZHI. Bioactivity and Mechanical Stability of Hydroxyapatite Ceramicsunder Micro-vibration Environment[J]. Journal of Inorganic Materials, 2019, 34(4): 417-424.

图/表 7

图1 HA10、HA20、HA35和HA多孔支架烧结后XRD图谱

Fig. 1 XRD patterns of HA10, HA20, HA35 and porous HA scaffolds

图2 HA材料的SEM照片

Fig. 2 SEM images of HA materials (a) HA10; (b) HA20; (c) HA35; (d) HA porous scaffold; (d1) Partial magnification of porous scaffoldss

图3 微振动对不同微孔隙率HA陶瓷片钙离子释放的影响

Fig. 3 Calcium release concentration of HA slices with different mass fraction (HA10, HA20, HA35) under 40 Hz and 60 Hz frequency and static state incubation environment at d3, d7 and d14 (* represents statistical difference, P<0.05)

图4 微振动对不同微孔隙率HA片蛋白吸附的影响

Fig. 4 Protein adsorption concentration of HA discs with different mass fraction (HA10, HA20, HA35) under 40 Hz and 60 Hz frequency and static state incubation environment at d1 and d3 (* represents statistical difference, P<0.05)

图5 HA在SBF溶液中3, 7 d后的SEM照片

Fig. 5 SEM results of HA ceramic discs with different mass fraction soaked in SBF solution for 3 and 7 d (a) Static state; (b) 40 Hz; (c) 60 Hz; 1: HA10-at d 3; 2: HA10- at d 7; 3: HA20- at d 3; 4: HA20- at d 7; 5: HA35- at d 3; 6: HA35- at d 7

图6 HA材料浸泡在SBF溶液中7 d后的EDS能谱分析

Fig. 6 EDS spectra of HA materials after 7 d in SBF solution (a) Static state; (b) 40 Hz; (c) 60 Hz; 1: HA10-at d 7; 2: HA20-at d 7; 3: HA35-at d 7

图7 微振动对HA多孔支架抗压强度的影响

Fig. 7 Compressive strength of HA porous scaffold under static state, 40 and 60 Hz vibration frequency incubated for 7 and 14 d

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