锰掺杂纳米羟基磷灰石的体外抗菌-促成骨性能研究

doi:10.15541/jim20230438

无机材料学报 ›› 2024, Vol. 39 ›› Issue (3): 313-320.DOI: 10.15541/jim20230438 CSTR: 32189.14.10.15541/jim20230438

所属专题：【生物材料】骨骼与齿类组织修复(202409)

锰掺杂纳米羟基磷灰石的体外抗菌-促成骨性能研究

李承瑜(), 丁自友, 韩颖超()

武汉理工大学材料复合新技术国家重点实验室, 武汉 430070

收稿日期:2023-09-24 修回日期:2023-10-15 出版日期:2024-03-20 网络出版日期:2023-11-22
通讯作者: 韩颖超, 研究员. E-mail: hanyingchao@whut.edu.cn
作者简介:李承瑜(1995-), 男, 博士研究生. E-mail: lcy9524@foxmail.com
基金资助:
国家自然科学基金(51672206)

In vitro Antibacterial and Osteogenic Properties of Manganese Doped Nano Hydroxyapatite

LI Chengyu(), DING Ziyou, HAN Yingchao()

State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan 430070, China

Received:2023-09-24 Revised:2023-10-15 Published:2024-03-20 Online:2023-11-22
Contact: HAN Yingchao, professor. E-mail: hanyingchao@whut.edu.cn
About author:LI Chengyu(1995-), male, PhD candidate. E-mail: lcy9524@foxmail.com
Supported by:
National Natural Science Foundation of China(51672206)

摘要/Abstract

摘要：

羟基磷灰石(HAP)作为一种常见的骨修复材料, 在治疗感染性骨缺损时仍面临着细菌感染的风险, 有限的骨诱导能力也阻碍了其进一步应用。本研究采用共沉淀法制备了一种锰掺杂羟基磷灰石纳米棒(MnHAP), 该纳米材料兼具优良的细胞生物相容性, 高抗菌效率和骨诱导能力。抗菌实验表明, MnHAP10(n(Mn)/n(Ca+Mn)=10%))对大肠杆菌和金黄色葡萄球菌的抑制率可分别达到77.85%和75.92%, 并且在808 nm近红外光照射下, 对大肠杆菌的抗菌效率得到了进一步增强(97.63%)。细胞增殖以及相关成骨基因实验表明, MnHAP有利于成骨细胞的增殖分化, 提高了对蛋白质的吸附能力和成骨活性, 促进了相关成骨基因的表达, 且具有良好的生物相容性。MnHAP纳米棒有望为感染性骨缺损治疗提供一种新的思路。

关键词: 羟基磷灰石, 锰, 抗菌, 感染性骨缺损, 光热

Abstract:

Hydroxyapatite (HAP), as a common bone repair material, still faces the risk of bacterial infection in the treatment of infectious bone defects, whose limited osteogenic properties also hinders its further application. This study used a coprecipitation method to prepare the manganese doped hydroxyapatite nanorod (MnHAP), which exhibited excellent cell biocompatibility, high antibacterial efficiency and osteogenic properties. Antibacterial experiments showed that the inhibition rates of MnHAP10(n(Mn)/n(Ca+Mn)=10%)) against Escherichia coli and Staphylococcus aureus can reach 77.85% and 75.92%, respectively. Moreover, the antibacterial efficiency of MnHAP10 against Escherichia coli can be further enhanced (97.63%) under 808 nm near-infrared light irradiation. Cell proliferation and related osteogenic gene experiments display that MnHAP is beneficial for the proliferation and differentiation of osteoblasts, which improves protein adsorption capacity, stimulates osteogenic activity, and promotes the expression of related osteogenic genes, demonstrating its good biocompatibility. Therefore, MnHAP nanorods are expected to provide a new approach in the treatment of infectious bone defects.

Key words: hydroxyapatite, manganese, antibacterial, infectious bone defect, photothermal

中图分类号:

TQ174

李承瑜, 丁自友, 韩颖超. 锰掺杂纳米羟基磷灰石的体外抗菌-促成骨性能研究[J]. 无机材料学报, 2024, 39(3): 313-320.

LI Chengyu, DING Ziyou, HAN Yingchao. In vitro Antibacterial and Osteogenic Properties of Manganese Doped Nano Hydroxyapatite[J]. Journal of Inorganic Materials, 2024, 39(3): 313-320.

图/表 6

图1 MnHAP的微观形貌和结构分析

Fig. 1 Morphologies and structures of MnHAP (a) XRD patterns of MnHAP and HAP; (b-f) SEM images of HAP (b), MnHAP1 (c), MnHAP5 (d), MnHAP10 (e), and MnHAP20 (f); (g-k) TEM images of HAP (g), MnHAP1 (h), MnHAP5 (i), MnHAP10 (j) and MnHAP20 (k); (l-p) Selected area diffraction pattern of HAP (l), MnHAP1 (m), MnHAP5 (n), MnHAP10 (o), and MnHAP20 (p)

表1 MnHAP和HAP的晶格参数, 晶胞体积和结晶度

Table 1 Lattice parameters, volume, and crystallinity of MnHAP and HAP

Sample	a-axis/Å	c-axis/Å	Volume/Å³	Crytallinity/%
HAP	9.41129	6.88743	528.31	61.77
MnHAP1	9.41083	6.88255	528.78	62.92
MnHAP5	9.40699	6.88027	527.74	61.72
MnHAP10	9.40415	6.87068	527.4	55.98
MnHAP20	9.40065	6.86074	527.11	50.36

图2 MnHAP的锰离子释放曲线

Fig. 2 Manganese ion release curves of MnHAP

图3 MnHAP10的Mn元素价态(a)、MnHAP光吸收(b)、MnHAP10光热转换效率(c)、MnHAP单纯抗菌作用(d~e)和MnHAP10光热抗菌作用(f)

Fig. 3 Mn element valence in MnHAP10 (a), photoabsorption of MnHAP (b), photothermal conversion efficiency of MnHAP10 (c), pure antibacterial effect MnHAP (d-e) and photothermal antibacterial MnHAP10 (f)

图4 MnHAP的比表面积与孔结构(a)、Zeta电位(b)与蛋白质吸附量(c)

Fig. 4 Specific surface area and pore structure (a), Zeta potential (b) and protein adsorption amount (c) of MnHAP

图5 MnHAP的细胞相容性(a)、成骨前期特征酶活性(b)以及特征蛋白和成骨相关基因表达(c~h)

Fig. 5 Cell compatibility (a), osteogenic marker (ALP/ARS) (b), and gene expression of protein marker and osteogenesis-related genes (c-h) of MnHAP

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锰掺杂纳米羟基磷灰石的体外抗菌-促成骨性能研究

In vitro Antibacterial and Osteogenic Properties of Manganese Doped Nano Hydroxyapatite

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