Electrospinning Gelatin/Chitosan/Hydroxyapatite/Graphene Oxide Composite Nanofibers with Antibacterial Properties

doi:10.15541/jim20140528

Abstract

Abstract:

Gelatin/chitosan/hydroxyapatite/graphene oxide composite nanofibers were prepared by electrospinning. The effect of composition on fiber morphology and antibacterial properties were investigated. The results show that increasing of gelatin concentration results in the increase of fiber diameter and then heavy fiber adhesion, showing ideal concentration scope of 15%-20%. Increasing chitosan concentration leads to thin fibers, with optimum concentration at 1%, while increasing hydroxyapatite (HA) concentration increases the ionic concentration of eletrospinning solution, leading to the decrease of beads and fiber adhesion. Nanofibers with smooth morphology are obtained when the diameter of HA particles is 12 μm and at concentration of 5%. And the addition of graphene oxide (GO) enhances uniform, smooth and antibacterial property of the composite nanofibers, which enables the gelatin/chitosan/hydrox- yapatite/GO fibers good antibacterial effect against both Staphylococcus aureus and Escherichia coli.

null

Key words: gelatin, chitosan, hydroxyapatite, graphene oxide, electrospun fibers, antibacterial property

CLC Number:

TQ318

LIANG Hong-Pei, WANG Ying-Bo, SU Zhi, LU Xiong, WANG Shuai. Electrospinning Gelatin/Chitosan/Hydroxyapatite/Graphene Oxide Composite Nanofibers with Antibacterial Properties[J]. Journal of Inorganic Materials, 2015, 30(5): 516-522.

References

[1] LI L H, WANG Y J, ZHU Y, et al. Poly (lactide-co-glycolide)/ hydroxyapatite nanofibrous scaffolds fabricated by electrospinning for bone tissue engineering. J. Mater. Sci.: Mater. Med., 2011, 22(8): 1873–1884.
[2] HOFMAN K, TUCKER N, STANGER J, et al. Effects of the molecular format of collagen on characteristics of electrospun fibres. J. Mater. Sci., 2012, 47(3): 1148–1155.
[3] JAISWAL A K, CHHABRA H, SONI V P, et al. Enhanced mechanical strength and biocompatibility of electrospun polycaprolactone-gelatin scaffold with surface deposited nano-hydroxyapatite. Mater. Sci. Eng., 2013, 33(4): 2376–2385.
[4] PEREZ M A, GUARINO V, CIRILLO V, et al. In vitro mineralization and bone osteogenesis in poly (ε-caprolactone)/gelatin nanofibers. J. Biomed. Mater. Res., 2012, 100(11): 3008–3019.
[5] WANG H Y, FENG Y K, FANG Z C, et al. Fabrication and characterization of electrospun gelatin-heparin nanofibers as vascular tissue engineering. Macromol. Res., 2013, 21(8): 860–869.
[6] MENG Z X, LI H F, SUN Z Z, et al. Fabrication of mineralized electrospun PLGA and PLGA/gelatin nanofibers and their potential in bone tissue engineering. Mat. Sci. Eng., 2013, 33(2): 699–706.
[7] SARAVANAN S, NETHALA S, PATTNAIK S, et al. Preparation, characterization and antimicrobial activity of a bio-composite scaffold containing chitosan/nano-hydroxyapatite/nano-silver for bone tissue engineering. Int. J. Bio. Macromol., 2011, 49(2): 188–193.
[8] OKUYAMA K, NOGUCHI K, HANAFUSA Y, et al. Structural study of anhydrous tendon chitosan obtained via chitosan/acetic acid complex. Int. J. Bio. Macromol., 1999, 26(4): 285–293.
[9] JAYAKUMAR R, PRABAHARAN M, NAIR S V, et al. Novel carboxymethyl derivatives of chitin and chitosan materials and their biomedical applications. Prog. Mater. Sci., 2010, 55(7): 675–709.
[10] MU Q X, SU G X, LI L W, et al. Size-dependent cell uptake of protein-coated graphene oxide nanosheets. ACS Appl. Mater. Inter., 2012, 4(4): 2259–2266.
[11] ARTILES M S, ROUT C S, FISHER T S. Graphene-based hybridmaterials and devices for biosensing. Adv. Drug. Deliver. Rev., 2011, 63(14/15): 1352–1360.
[12] WANG G M, QIAN F, SALTIKOV C W, et al. Microbial reduction of graphene oxide by shewanella. Nano Res., 2011, 4(6): 563–570.
[13] LU B G, LI T, ZHAO H T, et al. Graphene-based composite materials beneficial to wound healing. Nanoscale, 2012, 4(9): 2978–2982.
[14] FARIA A F, MARTINEZ D S, MEIRA S M, et al. Anti-adhesion and antibacterial activity of silver nanoparticlessupported on graphene oxide sheets. Colloid Surface B, 2014, 113: 115–124.
[15] CARPIO I E, SANTOS C M, WEI X, et al. Toxicity of a polymer- graphene oxide composite against bacterial planktonic cells, biofilms, and mammalian cells. Nanoscale, 2012, 4(15): 4746–4756.
[16] TITOV A V, KRAL P, PEARSON R. Sandwiched graphene-membrane superstructures. ACS Nano, 2010, 4(1): 229–234.
[17] GOENKA S, SANT V, SANT S. Graphene-based nanomaterials for drug delivery and tissue engineering. Journal of Controlled Release, 2014, 173: 75–88.
[18] SHI X T, CHANG H X, CHEN S, et al. Regulating cellular behavior on few-layer reduced graphene oxide films with well- controlled reduction states. Adv. Funct. Mater., 2012, 22(4): 751–759.
[19] REN H L, WANG C, ZHANG J L, et al. DNA cleavage system of nanosized graphene oxide sheets and copper ions. ACS Nano, 2010, 4(12): 7169–7174.
[20] SEABRA A B, PAULA A J, LIMA R, et al. Nanotoxicity of graphene and graphene oxide. Chem. Res. Toxicol., 2014, 27(2): 159–168.
[21] SHIRKHANZADEH M. Direct formation of hydroxyapatite on cathodically polarized electrodes. J. Mater. Sci.: Mater. Med., 1998, 9(2): 67–72.
[22] MOHAMED K R, MOSTAFA A A. Preparation and bioactivity evaluation of hydroxyapatite-titania/chitosan-gelatin polymeric biocomposites. Mat. Sci. Eng. C, 2008, 28(7): 1087–1099.
[23] CUI W G, LI X H, ZHOU S B, et al. Investigation on process parameters of electrospinning system through orthogonal experimental design. J. Appl. Polym. Sci., 2007, 103(5): 3105–3112.
[24] WANG Y B, LU X, LI D, et al. Hydroxyapatite/chitosan composite coatings on titanium supfaces by pulsed electrochemical deposition. Acta Polymerica Sinica, 2011(11): 1244–1252.
[25] 王策, 卢晓峰. 有机纳米功能材料: 高压静电纺丝技术与纳米纤维. 北京: 科学出版社, 2011: 42–44.
[26] 丁彬, 俞建勇. 静电纺丝与纳米纤维. 北京: 中国纺织出版社, 2011: 41–44.
[27] SEABRA A B, PAULA A J, LIMA R, et al. Nanotoxicity of graphene and graphene oxide. Chem. Res. Toxicol, 2014, 27(2): 159-168.
[28] AKHAVAN O, GHADERI E. Toxicity of graphene and graphene oxide nanowalls against bacteria. ACS nano, 2010, 4(10): 5731–5736.
[29] FENG L Z, LIU Z. Graphene in biomedicine: opportunities and challenges. Nanomedicine, 2011, 6(2): 317-324.
[30] KUILA T, BOSE S, KHANRA P, et al. Recent advances in graphene-based biosensors. Biosens Bioelectron, 2011, 26(12): 4637-4648.