Influence of Micro-nano Structure of Haydroxyapatite Particles on Protein Adsorption

doi:10.15541/jim20140510

Abstract

Abstract:

Hydroxyapatite (HAP) particles were hydrothermally synthesized with the surface morphologies adjusted by cyclohexane-1, 2, 3, 4, 5, 6-hexacarboxylic acid (H6E) as the template. HAP particles were characterized by XRD, BET, SEM and FTIR. The protein adsorption-desorption behaviors of positively charged lysozyme (LYS), fibrinogen (FN) and negatively charged bovine serum albumin (BSA) on these HAP particles were examined. The results indicate that using H6E as a template to fabricate micro-nano structures on HAP particles through hydrothermal reaction is simple and controllable. HAP particles with micro-nano structures show selective protein adsorption-desorption properties for different proteins. The protein-loaded shell-like HAP particle (HAP50-protein) shows an excellent protein release behavior in vitro.

null

Key words: hydroxyapatite, H6E, micro-nano structure, protein adsorption

CLC Number:

R318
TQ174

FU Ya-Kang, ZHOU Xue, XIAO Dong-Qin, SHI Feng, LU Xiao-Ying, WENG Jie. Influence of Micro-nano Structure of Haydroxyapatite Particles on Protein Adsorption[J]. Journal of Inorganic Materials, 2015, 30(5): 523-528.

References

[1] MASKARINEC S A, TIRRELL D A. Protein engineering approaches to biomaterials design. Current Opinion in Biotechnology, 2005, 16(4): 422–426.
[2] DUCHEYNE P, QIU Q. Bioactive ceramics: the effect of surface reactivity on bone formation and bone cell function. Biomaterials, 1999, 20(23/24): 2287–2303.
[3] LIAO X L, LU S Y, ZHUO Y, et al. Bone physiology, biomaterial and the effect of mechanical/physical microenvironment on mesenchymal stem cell osteogenesis. Cellular and Molecular Bioengineering, 2011, 4(4): 579–590.
[4] YOSHIMOTO H, SHIN Y M, TERAI H, et al. A biodegradable nanofiber scaffold by electrospinning and its potential for bone tissue engineering. Biomaterials, 2003, 24(12): 2077–2082.
[5] ZHANG H J , ZHU X D, WANG X L, et al. Effect of sintering processes on surface properties and protein adsorption of hydroxyapatite ceramic particles. Journal of Inorganic Materials, 2010, 25(7): 771–774.
[6] SANTOS E A, FARINA M, SOARES G A, et al. Surface energy of hydroxyapatite and β-tricalcium phosphate ceramics driving serum protein adsorptionand osteoblast adhesion. Mater. Sci. Mater. Med., 2008, 19: 2307–2316.
[7] ROUAHI M, CHAMPION E, GALLET O, et al. Physico-chemical characteristics and protein adsorption potential of hydroxyapatite particles: in?uence on biocompatibility of ceramics after sintering. Colloids and Surfaces B: Biointerfaces, 2006, 47(1): 10–19.
[8] YUAN H P, YANG Z J, LI Y B, et al. Osteoinduction by calcium phosphate biomaterials. Materials in Medicine, 1998, 9(12): 723–726.
[9] LORD M S, FOSS M, BESENBACHER F. Influence of nanoscale surface topography on protein adsorption and cellular response. Nanotoday February, 2010, 5(1): 66–78.
[10] FUJII E, OHKUBO M, TSURU K, et al. Selective protein adsorption property and characterization of nano-crystalline zinc-containing hydroxyapatite. Acta Biomater. 2006, 2(1): 69–74.
[11] ELANGOVAN S , MARGOLIS H C, OPPENHEIM F G, et al. Conformational changes in salivary proline-rich protein 1 upon adsorption to calcium phosphate crystals. Langmuir, 2007, 23(22): 11200–11205.
[12] XIAO D Q, SHI F, YAO N, et al. Nanostructured hydroxyapatite microspheres by templated synthesis: formation and mechanisms. Progress in Biochemistry and Biophysics, 2013, 40(10): 935–947.
[13] JOHNSON M, RICHARDSON C F, SALLIS J D, et al. Adsorption and mineralization effects of citrate and phosphocitrate on hydroxyapatite. Calcified Tissue International, 1991, 49(2): 134–137.
[14] JOKI? B, MITRI? M, RADMILOVI? V, et al. Synthesis and characterization of monetite and hydroxyapatite whiskers obtained by a hydrothermal method. Ceramics International, 2011, 37(1): 167–173.
[15] KOUTSOPOULOS S. Synthesis and characterization of hydroxyapatite crystals: a review study on the analytical methods. Journal of Biomedical Materials Research, 2002, 62(4): 600–612.
[16] ARAI T, NORDE W. The behavior of some model proteins at solid-liquid interfaces 1. adsorption from single protein solutions. Colloids and Surface, 1990, 51: 1–15.
[17] KANDORI K, SHIMIZU T, YASUKAWA A, et al. Adsorption of bovine serum albumin onto synthetic calcium hydroxyapatite: influence of particle texture. Biointerfaces, 1995, 5(1/2): 81–87.
[18] KANDORI K, FUDO A, ISHIKAWA T. Study on the paritcle texture dependence of protein adsorption by using synthetic micrometer-sized calcium hydroxyapatite particles. Colloids and Surfaces B: Biointerfaces, 2002, 24(2): 145–153.
[19] WANG Y S, HASSAN M S, GUNAWAN P, et al. Polyelectroly temediated formation of hydroxyapatite microspheres of controlled size and hierarchical structure. Journal of Colloid and Interface Science, 2009, 339(1): 69–77.
[20] SUN R X, CHEN K Z, LU Y P. Fabrication and dissolution behavior of hollow hydroxyapatite microspheres intended for controlled drug release. Materials Research Bulletin, 2009, 44(10): 1939–1942.
[21] AMOOZGAR Z, YEO Y. Recent advances in stealth coating of nanoparticle drug delivery systems. Nanomedicine and Nanobiotechnology, 2012, 4(2): 219–233.