无机材料学报 ›› 2023, Vol. 38 ›› Issue (7): 830-838.DOI: 10.15541/jim20220662 CSTR: 32189.14.10.15541/jim20220662

所属专题: 【生物材料】骨骼与齿类组织修复(202409)

• 研究快报 • 上一篇    下一篇

双相磷酸镁钙微球体外成血管和促成骨研究

吴未1,2(), BAKHET Shahd2, ASANTE Naomi Addai2, KAREEM Shefiu2, KOMBO Omar Ramadhan3, 李宾斌2, 戴红莲1,2()   

  1. 1.武汉理工大学 深圳研究院, 深圳 518000
    2.武汉理工大学 材料复合新技术国家重点实验室, 湖北省生物材料工程研究中心, 武汉 430070
    3.姆贝亚科技大学 医学科学与技术系, 姆贝亚, 坦桑尼亚
  • 收稿日期:2022-11-05 修回日期:2022-12-18 出版日期:2023-03-20 网络出版日期:2023-03-20
  • 通讯作者: 戴红莲, 教授. E-mail: daihonglian@whut.edu.cn
  • 作者简介:吴 未(1998-), 女, 硕士. E-mail: 2625276216@qq.com

In vitro Study of Biphasic Calcium Magnesium Phosphate Microspheres for Angiogenesis and Bone Formation

WU Wei1,2(), BAKHET Shahd2, ASANTE Naomi Addai2, KAREEM Shefiu2, KOMBO Omar Ramadhan3, LI Binbin2, DAI Honglian1,2()   

  1. 1. Shenzhen Institute of Wuhan University of Technology, Shenzhen 518000, China
    2. State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Biomedical Materials and Engineering Research Center of Hubei Province, Wuhan University of Technology, Wuhan 430070, China
    3. Department of Medical Science and Technology, Mbeya University of Science and Technology, Mbeya, Tanzania
  • Received:2022-11-05 Revised:2022-12-18 Published:2023-03-20 Online:2023-03-20
  • Contact: DAI Honglian, professor. E-mail: daihonglian@whut.edu.cn
  • About author:WU Wei (1998-), male, Master. E-mail: 2625276216@qq.com
  • Supported by:
    National Key Research and Development Program of China(2022YFB4601402);National Natural Science Foundation of China(32201109);Guangdong Basic and Applied Basic Research Foundation(2021A1515110557);Foshan Xianhu Laboratory of the Advanced Energy Science and Technology Guangdong Laboratory(XHT2020-008)

摘要:

磷酸三钙(β-TCP)陶瓷替代材料由于其与骨矿物成分相近及良好的生物相容性和骨传导性, 近年来被广泛关注, 常以纳米颗粒、支架和微球等形式用于骨修复。本研究制备了五种不同的磷酸三钙/磷酸三镁(TMP) (TCP、25% TMP、50% TMP、75% TMP和TMP)复合微球并作了相应表征。随着复合微球中TMP含量增加, 微球释放的Mg2+和Ca2+的累积浓度增加, 且TMP可以调节复合微球的降解速率。以小鼠胚胎成骨细胞前体细胞(MC3T3-E1)和人脐静脉内皮细胞(HUVECs)为模型, 评价了该复合微球的生物相容性、成血管和成骨作用。结果表明, 与TCP、TMP和75% TMP相比, 25% TMP和50% TMP复合微球具有更好的细胞相容性, 对HUVECs有一定的促增殖作用。因此, 含25% TMP和50% TMP的复合微球对血管生成和成骨具有更积极的作用。

关键词: β-TCP, TMP, 骨缺损, 陶瓷, 微球

Abstract:

Beta tricalcium phosphate (β-TCP) ceramic substituted materials have attracted a large amount of attention in the last decades because of their chemical similarity with bone inorganic components, good biocompatibility, and osteoconductivity. Such materials can be used for bone replacement and bone formation in various forms, such as nanoparticles, scaffolds and microspheres. In this study, five different microsphere materials of tricalcium phosphate/trimagnesium phosphate (TMP) (TCP, 25% TMP, 50% TMP, 75% TMP, and TMP) composites were prepared and characterized. With the increase of TMP content in the composite microspheres, the cumulative concentration of Mg2+ and Ca2+ released from the microspheres increased, indicating that TMP can regulate the degradation rate of the composite microspheres. The osteoblast precursor cell line (MC3T3-E1 cells) and human umbilical vein endothelial cells (HUVECs) were used as models to evaluate the biocompatibility, angiogenesis and osteogenesis of the composite microspheres. The results showed that compared with TCP, TMP and 75% TMP group, 25% TMP and 50% TMP composite microspheres had better cell compatibility and had a certain proliferative effect on HUVECs. Therefore, composite microspheres of 25% TMP and 50% TMP have more significant positive effects on angiogenesis and osteogenesis.

Key words: β-TCP, TMP, bone defect, ceramics, microsphere

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