羟基磷灰石对海藻酸钙水凝胶的制备及性能的影响

doi:10.3724/SP.J.1077.2010.01087

无机材料学报 ›› 2010, Vol. 25 ›› Issue (10): 1087-1091.DOI: 10.3724/SP.J.1077.2010.01087 CSTR: 32189.14.SP.J.1077.2010.01087

羟基磷灰石对海藻酸钙水凝胶的制备及性能的影响

孙静, 肖玉梅, 范红松, 张兴栋

(四川大学国家生物医学材料工程技术研究中心, 成都610064)

收稿日期:2010-01-22 修回日期:2010-03-04 出版日期:2010-10-20 网络出版日期:2010-09-26
基金资助:
国家重点基础研究发展计划(973计划)(2005CB623901)

Effect of Hydroxyapatite on the Preparation and Properties of Alginate Hydrogel

SUN Jing, XIAO Yu-Mei, FAN Hong-Song, ZHANG Xing-Dong

(National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, China)

Received:2010-01-22 Revised:2010-03-04 Published:2010-10-20 Online:2010-09-26
Supported by:
973 Programe(2005CB623901)

摘要/Abstract

摘要： 采用具有良好生物相容性的羟基磷灰石(HA)作为钙源, 开发了一种新型的HA-GDL交联体系制备海藻酸钙水凝胶. 通过调节海藻酸钠溶液的浓度CSA和钙离子与海藻酸钠中羧基的摩尔比f, 分别制备CSA=2%、2.5%、3%, f=0.27、0.36、0.54的一系列水凝胶. 以CaCO₃-GDL体系作为对照, 系统比较了两种体系制得的水凝胶在凝胶化时间、凝胶形貌、力学性能以及溶胀收缩等方面的性能. 结果显示, 相同凝胶条件下, 采用HA-GDL体系制得的海藻酸钙水凝胶具有较快的凝胶化速率, 较为均一的凝胶结构, 较强的力学性能和较低的溶胀率. 将海藻酸钙凝胶与软骨细胞复合进行体外培养, 结果显示该体系具有良好的细胞相容性, 从而说明该材料体系可能是一种良好的软骨组织工程支架材料.

关键词: 海藻酸, 水凝胶, 羟基磷灰石, 碳酸钙, 组织工程

Abstract: A novel crosslingking system, hydroxyapatite-D-glucono-δ-lactone (HA-GDL) was developed to prepare alginate hydrogel, in which calcium ions can be released to initiate gelation. A series of hydrogels were prepared by adjusting the sodium alginate (SA) concentration and the molar ratio of calcium ion in HA to carboxyl of SA. Gelation time, surface morphology, mechanical property and swelling capacity of hydrogels crosslinked by HA-GDL system were characterized and compared with that crosslinked by the calcium carbonate- D-glucono-δ-lactone (CaCO₃-GDL) system. The results show that under the same conditions, the hydrogels obtained from the HA-GDL system have faster gelation rate, more uniform structure, stronger mechanical strength and lower swelling ratio compared with that obtained from CaCO₃-GDL system. Chondrocytes of newborn rabbit are embedded in alginate hydrogels to construct tissue engineered cartilage in vitro. Primary results show that the alginate hydrogels prepared with HA-GDL system have good cell compatibility and are with potential to be used as a scaffold for cartilage repair.

Key words: alginate, hydrogel, hydroxyapatite, calcium carbonate, tissue engineering

中图分类号:

R318

孙静, 肖玉梅, 范红松, 张兴栋. 羟基磷灰石对海藻酸钙水凝胶的制备及性能的影响[J]. 无机材料学报, 2010, 25(10): 1087-1091.

SUN Jing, XIAO Yu-Mei, FAN Hong-Song, ZHANG Xing-Dong. Effect of Hydroxyapatite on the Preparation and Properties of Alginate Hydrogel[J]. Journal of Inorganic Materials, 2010, 25(10): 1087-1091.

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羟基磷灰石对海藻酸钙水凝胶的制备及性能的影响

Effect of Hydroxyapatite on the Preparation and Properties of Alginate Hydrogel

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