Mn/nHA涂层CF/PEEK复合材料的制备及体外成骨活性评价

doi:10.15541/jim20250078

摘要/Abstract

摘要： 碳纤维/聚醚醚酮（CF/PEEK）在骨修复领域已得到了广泛应用,但其生物惰性致使其骨整合性较弱,极大地影响了临床应用的长期稳定性和骨修复效果。本研究基于无机纳米粒子液相自组装策略,在CF/PEEK表面构筑了锰掺杂纳米羟基磷灰石（Mn/nHA）涂层,以期改善其生物活性和骨整合性。实验结果表明,Mn/nHA和nHA涂层均显著改善CF/PEEK表面粗糙度和亲水性。进一步的体外研究发现,Mn/nHA和nHA两组涂层改性材料在与大鼠间充质干细胞共培养第7天后,相对细胞存活率比空白对照组分别高达180%和159%,均表现出显著的促增殖效果。此外,茜素红染色和逆转录聚合酶链式反应检测发现两种涂层均能增强细胞在材料表面的矿化和成骨分化,且Mn/nHA涂层呈现了更强的促进作用。

关键词: 羟基磷灰石, 锰, 碳纤维/聚醚醚酮, 体外成骨活性

Abstract: Carbon fiber/polyetheretherketone (CF/PEEK) has been widely used in the field of bone repair. However, its bio-inert nature results in poor osseointegration, which significantly limits its long-term stability and bone repair efficacy in clinical applications. Here, a manganese-doped nano-hydroxyapatite (Mn/nHA) coating was constructed on the surface of CF/PEEK using a liquid-phase self-assembly strategy of inorganic nanoparticles. The experimental results demonstrated that both Mn/nHA and nHA coatings significantly improved the surface roughness and hydrophilicity of CF/PEEK. Further in vitro studies revealed that after 7 days of co-culture with rat mesenchymal stem cells, the relative cell viability of the Mn/nHA and nHA-coated materials reached as high as 180% and 159%, respectively, compared to the blank control, indicating a pronounced pro-proliferative effect. Additionally, alizarin red staining and reverse transcription-polymerase chain reaction (RT-PCR) analysis showed that both coatings enhanced cell mineralization and osteogenic differentiation on the material surface, with the Mn/nHA coating exhibiting a stronger promoting effect.

Key words: hydroxyapatite, manganese, CF/PEEK, osteogenic activity

中图分类号:

R318

李正浩, 李婧鸣, 章玉祥, 袁波, 张凯, 朱向东. Mn/nHA涂层CF/PEEK复合材料的制备及体外成骨活性评价[J]. 无机材料学报, DOI: 10.15541/jim20250078.

LI Zhenghao, LI Jingming, ZHANG Yuxiang, YUAN Bo, ZHANG Kai, ZHU Xiangdong. Preparation and in vitro Osteogenic Activity Evaluation of Mn/nHA Coated CF/PEEK Composite[J]. Journal of Inorganic Materials, DOI: 10.15541/jim20250078.

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