Abstract: Lead selenide nanoclusters and nanocubes were successfully in situ prepared through a room-temperature biosubstrate-induced approach employing the Pb(Ac)2 solution, Na₂SeSO₃ solution as the reactants, and the eggshell membrane (ESM), which contains many unique and periodically distributed functional groups, as the live biomaterial. Results show that under the complexation and electronic roles of these macromolecules with inorganic precursor ions, PbSe nanocrystallites firstly process oriented-formation, controlled-congregation and periodic-distribution on the mantle of ESM fibers, then they are self-assembled into the uniform nanoclusters with well crystalline, which are orderly distributed on the surface of ESM. The results of UV-Vis and PL analysis also indicate PbSe nanoparticles prepared are with fine, uniform size and nice distribution.

Key words: lead selenide, nanoclusters, eggshell membrane, single crystal