Photo/Magnetic Thermal Fe2SiO4/Fe3O4 Biphasic Bioceramic and Its Composite Electrospun Membrane: Preparation and Antibacterial

doi:10.15541/jim20210659

Abstract

Abstract:

Biomaterials with both antibacterial and tissue repair activity have promising applications in the field of regenerative medicine. Both photothermal and magnetothermal-based techniques have antimicrobial effects, but the limited penetration capacity of light and the low thermal conversion efficiency of magnetothermal reagents limit their applications in biomedical fields. Here, we synthesized Fe₂SiO₄/Fe₃O₄ biphasic composite bioceramic powders, which not only display good photothermal and magnetothermal effects, but also effectively release active Fe and silicate ions, and prepared electrospun membranes by combining ceramic powders with gelatin/polycaprolactone, which not only exhibit good cytocompatibility, but also, importantly, possess both photothermal and magnetothermal properties. The composite membranes, under conditions of being irradiated with near-infrared light (808 nm, 0.36 W·cm^-2) and placed in alternating magnetic field (506 kHz, 837 A·m^-1) for 15 min simultaneously, are able to inhibit bacterial activity more effectively than the thermal treatment with near-infrared light or magnetic field alone. Therefore, this Fe-Si-based bioceramic and its composite material with photothermal, magnetothermal, antimicrobial, and biocompatible properties, have potential application in the field of regenerative medicine.

Key words: Fe, silicate, biphasic bioceramic, electrospun, antibacterial

CLC Number:

TQ174

SHENG Lili, CHANG Jiang. Photo/Magnetic Thermal Fe₂SiO₄/Fe₃O₄ Biphasic Bioceramic and Its Composite Electrospun Membrane: Preparation and Antibacterial[J]. Journal of Inorganic Materials, 2022, 37(9): 983-990.

Figures/Tables 11

Fig. 1 TG-DTA curves of gel powders tested in (a) air and (b) argon atmosphere

Fig. 2 XRD patterns of FF products calcined at 800 ℃ in different atmospheres FF-1: the product obtained by calcining without argon gas; FF-2-FF-4: the product obtained by calcining with argon gas at 10, 25 and >25 kPa in the furnace, respectively.

Table 1 Ion release of powders prepared under different conditions after 24 h being submersed in cell culture medium ECM

Powder	Fe ion/(μg·mL^-1)	Silicate ion/(μg·mL^-1)
FF-1	43.96	70.12
FF-2	28.37	40.23
FF-3	13.62	27.16
FF-4	5.76	12.68

Fig. 3 Magnetothermal and photothermal properties of powder products after being calcined under different conditions (a) Magnetic analysis results; (b) Results of thermal performance of different powders under alternating magnetic field intensity of 506 kHz at 837 A·m-1; (c) Photothermal performance of different powders under 808 nm near-infrared light irradiation at a density of 0.36 W·cm-2. FF-1: the product obtained by calcining without argon gas; FF-2-FF-4: the product obtained by calcining with argon gas at 10, 25 and >25 kPa in the furnace, respectively. 1 emu=103 A·m-1, 1 Oe=1000/4π A/m Colorful figures are available on website

Fig. 4 Particle size characterization of FF-2 powders

Fig. 5 SEM and TEM images of FF-2 powders (a, b) SEM images at low (a) and high (b) magnification; (c) TEM high-resolution image of the powder with inset showing electron diffraction pattern of the powder

Fig. 6 Morphologies of composite electrospun membranes with different FF-2 powder compositing amounts (a) Optical photos; (b) SEM images. 0, 10, 20, 30, and 40 in the figures represent composite membranes with powder contents of 0, 10%, 20%, 30%, and 40%, respectively

Table 2 Ion release properties of composite electrospun films with different powder contents in cell culture medium ECM

Powder content/%	Fe ion/(μg·mL^-1)	Silicate ion/(μg·mL^-1)
0	0	0
10	0.05	0.27
20	0.13	1.45
30	0.32	2.76
40	0.61	5.98

Fig. 7 Photothermal and magnetothermal properties of composite membranes with different powder contents (a) Under NIR light (0.36 W·cm-2) irradiation at a wavelength of 808 nm; (b) In an alternating magnetic field (506 kHz, 837A·m-1); (c) Composite film with 30% powder content under laser, alternating magnetic field and laser in combination with alternating magnetic field, respectively. 0, 10, 20, 30, and 40 in the figures represent composite membranes with powder contents of 0, 10%, 20%, 30%, and 40%, respectively. MTT: magnetothermal; PTT: photothermal; MTT+PTT: combined photothermal and magnetothermal

Fig. 8 Cytocompatibility of FG-30 composite electrospun membranes (a-f) SEM images of cell adhesion on FG-0 membrane (a-c) and FG-30 composite membrane (d-f) after 24 h of HUVEC culture, respectively; (g) Effect of composite membrane on HUVECs proliferation. Blank, FG-0, and FG-30 in the figures indicate blank control group, FG-0 electrospun membrane group, and FG-30 composite electrospun membrane group, respectively. * indicates p<0.05 which means significant difference between groups

Fig. 9 Combined photo/magneto-thermal inhibition of S.aureus by FG-30 composite membrane (a) Optical photographs of colony coated plates; (b) Statistical results of inhibition rate. Blank, FG-0, FG-30, FG-30 (MTT), FG-30 (PTT), and FG-30 (MTT+PTT) denote blank control, FG-0 membrane, FG-30 membrane, FG-30 membrane with magnetothermal, FG-30 membrane with photothermal, FG-30 membrane with combined photothermal and magnetothermal, respectively. * indicates p<0.05 which means significant difference between groups

References 26

[1]	BELKAID Y, TAMOUTOUNOUR S. The influence of skin microorganisms on cutaneous immunity. Nature Reviews Immunology, 2016, 16(6): 353-366. DOI URL
[2]	FALANGA V. Wound healing and its impairment in the diabetic foot. Lancet, 2005, 366(9498): 1736-1743. DOI URL
[3]	ROBSON M. Wound infection - a failure of wound healing caused by an imbalance of bacteria. Surgical Clinics of North America, 1997, 77(3): 637-650. DOI URL
[4]	LI P L, HAN F X, CAO W W, et al. Carbon quantum dots derived from lysine and arginine simultaneously scavenge bacteria and promote tissue repair. Applied Materials Today, 2020, 19(1): 100601. DOI URL
[5]	XIE X, WU J R, CAI X J, et al. Photothermal/pH response B-CuS-DOX nanodrugs for chemo-photothermal synergistic therapy of tumor. Journal of Inorganic Materials, 2020, 36(1): 81-87. DOI URL
[6]	ZENG Y L, CHEN J J, TIAN Z F, et al. Preparation of mesoporous organosilica-based nanosystem for in vitro synergistic chemo-and photothermal therapy. Journal of Inorganic Materials, 2020, 35(12): 1365-1372. DOI URL
[7]	ZHAO L Y, LIU Y M, XING R R, et al. Supramolecular photothermal effects: a promising mechanism for efficient thermal conversion. Angewandte Chemie International Edition, 2020, 59(10): 3793-3801. DOI URL
[8]	WU G Z, WU Z Y, LIU L, et al. NIR light responsive MoS₂ nanomaterials for rapid sterilization: optimum photothermal effect via sulfur vacancy modulation. Chemical Engineering Journal, 2022, 427(1): 132007. DOI URL
[9]	CHEN R, ROMERO G, CHRISTIANSEN M G, et al. Wireless magnetothermal deep brain stimulation. Science, 2015, 347(6229): 1477-1480. DOI URL
[10]	LI W, WEI W Y, WU X P, et al. The antibacterial and antibiofilm activities of mesoporous hollow Fe₃O₄ nanoparticles in an alternating magnetic field. Biomaterials Science, 2020, 8(16): 4492-4507. DOI URL
[11]	ZHUANG H, LIN R C, LIU Y Q, et al. 3D-printed bioceramic scaffolds with osteogenic activity for simultaneous photo-magnetothermal therapy of bone tumor. ACS Biomaterials Science and Engineering, 2019, 5(12): 6725-6734. DOI URL
[12]	FU D P, LIU J L, REN Q L, et al. Magnetic iron sulfide nanoparticles as thrombolytic agents for magnetocaloric therapy and photothermal therapy of thrombosis. Frontiers in Materials, 2019, 6(1): 316. DOI URL
[13]	LIU J C, GUO X, ZHAO Z, et al. Fe₃S₄ nanoparticles for arterial inflammation therapy: integration of magnetic hyperthermia and photothermal treatment. Applied Materials Today, 2020, 18(1): 100457. DOI URL
[14]	LI H Y, CHANG J. Stimulation of proangiogenesis by calcium silicate bioactive ceramic. Acta Biomaterialia, 2013, 9(2): 5379-5389. DOI URL
[15]	ZHAI W Y, LU H X, CHEN L, et al. Silicate bioceramics induce angiogenesis during bone regeneration. Acta Biomaterialia, 2012, 8(1): 341-349. DOI URL
[16]	YU Q Q, CHANG J, WU C. Silicate bioceramics: from soft tissue regeneration to tumor therapy. Journal of Material Chemistry B, 2019, 7(36): 5449-5460. DOI URL
[17]	MAO L X, XIA L G, CHANG J, et al. The synergistic effects of Sr and Si bioactive ions on osteogenesis, osteoclastogenesis and angiogenesis for osteoporotic bone regeneration. Acta Biomaterialia, 2017, 61(1): 217-232. DOI URL
[18]	GAO L, ZhOU Y L, PENG J L, et al. A novel dual-adhesive and bioactive hydrogel activated by bioglass for wound healing. NPG Asia Materials, 2019, 11: 66.
[19]	WEINTRAUB L R, GORAL A, GRASSO J, et al. Collagen biosynthesis in iron overload. Annals of the New York Academy of Sciences, 1988, 526(1): 179-184.
[20]	SHENG L, ZHANG Z, ZHANG Y, et al. A novel “hot spring”-mimetic hydrogel with excellent angiogenic properties for chronic wound healing. Biomaterials, 2020, 264(1): 120414. DOI URL
[21]	XING M, HUAN Z G, LI Q, et al. Containerless processing of Ca-Sr-Si system bioactive materials: thermophysical properties and ion release behaviors. Ceramics International, 2017, 43(6): 5156-5163. DOI URL
[22]	LI H Y, CHANG J. Bioactive silicate materials stimulate angiogenesis in fibroblast and endothelial cell co-culture system through paracrine effect. Acta Biomaterialia, 2013, 9(6): 6981-6991. DOI URL
[23]	XING M, WANG X Y, WANG E D, et al. Bone tissue engineering strategy based on the synergistic effects of silicon and strontium ions. Acta Biomaterialia, 2018, 72(1): 381-395. DOI URL
[24]	ZHANG J, SHI H S, LIU J Q, et al. Good hydration and cell-biological performances of superparamagnetic calcium phosphate cement with concentration-dependent osteogenesis and angiogenesis induced by ferric iron. Journal of Materials Chemistry B, 2015, 3(45): 8782-8795. DOI URL
[25]	WU M, DEOKAR A R, LIAO J, et al. Graphene-based photothermal agent for rapid and effective killing of bacteria. ACS Nano, 2013, 7(2): 1281-1290. DOI URL
[26]	IBELLI T, TEMPLETON S, LEVI-POLYACHENKO N. Progress on utilizing hyperthermia for mitigating bacterial infections. International Journal of Hyperthermia, 2018, 34(2): 144-156. DOI URL

Photo/Magnetic Thermal Fe₂SiO₄/Fe₃O₄ Biphasic Bioceramic and Its Composite Electrospun Membrane: Preparation and Antibacterial

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