Bioinspired Polydopamine Functionalization of Titanium Surface for Silver Nanoparticles Immobilization with Antibacterial Property

doi:10.15541/jim20140162

Abstract

Abstract:

A bioinspired polydopamine (PDA) layer was deposited on titanium surface by simply dipping the substrate into an alkaline dopamine solution. The in vitro bioactivity of the polydopamine coated titanium was assessed by incubation in simulated body fluids (SBF). The results showed that surface-anchored catecholamine moieties in polydopamine enriched the interface with calcium ions, facilitating the deposition of hydroxyapatite. Silver nanoparticles (AgNPs) were metallised on the PDA-grafted titanium surface in mild aqueous environments. About 14% silver release as Ag⁺ ions were found after 5 d when the surfaces were exposed to deionized water. The AgNPs showed an efficient microbicidal activity against Staphylococcus Aureus bacterial strains, while not significantly affecting osteoblast cells (MC3T3-E1) viability.

Key words: titanium, polydopamine, silver nanoparticles, antibacterial property, cytocompatibility

CLC Number:

TB331

TAN Ying, Tan Guo-Xin, NING Cheng-Yun, RONG Xi-Cang, ZHANG Yu, ZHOU Lei. Bioinspired Polydopamine Functionalization of Titanium Surface for Silver Nanoparticles Immobilization with Antibacterial Property[J]. Journal of Inorganic Materials, 2014, 29(12): 1320-1326.

Figures/Tables 6

Fig. 1 SEM images (a-c) and water contact angle (d) of Ti (a), pTi (b) and Ti-PDA(c)

Fig. 2 SEM images, EDS spectra (a-c) and FTIR spectra (d) of Ti (a), pTi (b) and Ti-PDA (c) after 5 d in simulated body fluid

Fig. 3 SEM (a), XPS (b), Raman spectrum (c) and release of Ag+ (d) of Ti-PDA-Ag

Fig. 4 Typical photographs of re-cultivated Staphylococcus Aureus colonies on agar Ti (a), pTi (b), Ti-PDA (c) and Ti-PDA-Ag (d)

Fig. 5 SEM images of the Ti (a), pTi (b), Ti-PDA (c) and Ti-PDA-Ag (d) surfaces after incubation with Staphylococcus Aureus for 6 h

Fig. 6 SEM images showing the morphology of MC3T3-E1 cells after 12 h of culture on Ti (a), pTi(b), Ti-PDA (c), Ti-PDA -Ag (d) and MC3T3-E1 proliferation measured using MTT after incubation for 1, 3, 5 and 7 d (n=5, p< 0.05 ) (e) *represents significant difference

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