C12A7-O-材料抗菌性能及机理研究

doi:10.3724/SP.J.1077.2010.00942

无机材料学报 ›› 2010, Vol. 25 ›› Issue (9): 942-946.DOI: 10.3724/SP.J.1077.2010.00942 CSTR: 32189.14.SP.J.1077.2010.00942

C12A7-O^-材料抗菌性能及机理研究

宫璐, 沈静, 李全新

(中国科学技术大学化学物理系, 合肥230026)

收稿日期:2010-01-11 修回日期:2010-02-24 出版日期:2010-09-20 网络出版日期:2010-08-25
基金资助:
国家“863”项目(2009AA05Z435); 国家“973”项目(2007CB210206); 国家自然科学基金(50772107)

Investigation on the Antibacterial Activity and Mechanism of C12A7-O^- Particles

GONG Lu, SHEN Jing, LI Quan-Xin

(Department of Chemical Physics, University of Science and Technology of China, Hefei 230026, China)

Received:2010-01-11 Revised:2010-02-24 Published:2010-09-20 Online:2010-08-25

摘要/Abstract

摘要：

使用气固反应方法制备了[Ca₂₄Al₂₈O₆₄]⁴⁺·4O^-(C12A7-O^-)粉末材料, 运用X射线衍射(XRD)和电子自旋共振(ESR)对抗菌实验前后C12A7-O^-材料结构特性的变化进行了表征, 研究了C12A7-O^-材料在溶液中对大肠杆菌E. Coli的抗菌性能. 实验结果表明, C12A7-O^-材料中含有的O^-浓度约为2×10²⁰ /cm3. 1 mg/mL的C12A7-O^-水溶液中, O^-浓度约为4×10¹⁶ /cm3. 当菌液浓度为10⁵ cfu/mL时, 1 mg/mL的C12A7-O^-水溶液可在1 h内将大肠杆菌浓度降低99.98%. 通过对比实验和分析发现, C12A7-O^-粉末释放的O?离子是C12A7-O^-溶液具有抗菌作用的重要因素之一.

关键词: C12A7, 抗菌性能, 抗菌机理

Abstract:

The [Ca₂₄Al₂₈O₆₄]⁴⁺·4O^-(C12A7-O^-) particle was firstly investigated its antibacterial activity against bacteria E. Coli in aqueous solution and phosphate buffered saline (PBS). The C12A7-O^- particles were synthesized by gas-solid reaction method with CaCO₃ and γ-Al₂O₃ in a molar ratio of 12:7. The structure change of the C12A7-O^- particles was measured by X-ray diffraction (XRD) and electron spin resonance (ESR) after antibacterial test. The results show that the structure of the C12A7-O^- particles is completely changed into the structure of (CaO)₃Al₂O₃·6H₂O and Al(OH)₃. In the C12A7-O^- solution (1 mg/mL), the concentration of atomic oxygen anion (O^-) in the solution is 4×10¹⁶cm^-3. In the antibacterial test with the particle concentration of 3 mg/mL in aqueous solution, the bacteria concentration in decreased by 99.98% in 1 h from the original concentration of 10⁵cfu/mL. When the particle concentration is 1 mg/mL in PBS, the pH is 7.4 and the pH effect of the solution could be neglected, but the bacteria concentration still deduces to 3.7×10³ cfu/mL from the original concentration in 24 h. In briefly, O^- is the main factor in the antibacterial test in the C12A7-O^- PBS solution with the neutral pH environment.

Key words: C12A7, antibacterial activity, antibacterial mechanism

中图分类号:

TQ455

宫璐, 沈静, 李全新. C12A7-O^-材料抗菌性能及机理研究[J]. 无机材料学报, 2010, 25(9): 942-946.

GONG Lu, SHEN Jing, LI Quan-Xin. Investigation on the Antibacterial Activity and Mechanism of C12A7-O^- Particles[J]. Journal of Inorganic Materials, 2010, 25(9): 942-946.

参考文献

[1] 李吉东, 李玉宝, 王学江, 等(LI Ji-Dong, et al). 载铜锌纳米经基磷灰石的抗菌性能及机理研究. 无机材料学报(Journal of Inorganic Materials), 2006, 21(1): 162-168.
[2] 陈君华, 王飞, 程年寿, 等(CHEN Jun-Hua, et al). 铜原位改性HMS材料的表征及抗菌性能. 无机材料学报(Journal of Inorganic Materials), 2009, 24(4): 695-701.
[3] Cioffi N, Torsi L, Ditaranto N, et al. Copper nanoparticle/polymer composites with antifungal and bacteriostatic properties. Chem. Mater., 2005, 17(21): 5255-5262.
[4] 戴晋明, 侯文生, 魏丽乔, 等(DAI Jin-Ming, et al). 载银锌4A沸石抗菌剂抗变色性能的研究. 无机材料学报(Journal of Inorganic Materials), 2008, 23(5): 1011-1015.
[5] Lu Z X, Zhou L, Zhang Z L, et al. Cell damage induced by photocatalysis of TiO2 thin films. Langmuir, 2003, 19(21): 8765-8768.
[6] Jones N, Ray B, Ranjit K T, et al. Antibacterial activity of ZnO nanoparticle suspensions on a broad spectrum of microorganisms. FEMS Microbiol. Lett., 2008, 279(1): 71-76.
[7] Li Q X, Nishioka M, Kashiwagi H, et al. High-intensity atomic oxygen radical anion emission mechanism from 12CaO?7Al2O3 crystal surface. Surf. Sci., 2003, 527(1/2/3): 100-112.
[8] Wang L, Gong L, Zhao E, et al. Inactivation of Escherichia coli by O- water. Lett. Appl. Microbiol., 2007, 45(2): 200-205.
[9] Wang L, Yan L F, Zhao P T, et al. Surface modification of polystyrene with atomic oxygen radical anions-dissolved solution. Appl. Surf. Sci., 2008, 254(13): 4191-4200.
[10] Hayashi K, Matsuishi S, Kamiya T, et al. Light-induced conversion of an insulating refractory oxide into a persistent electronic conductor. Nature, 2002, 419(6906): 462-465.
[11] Miyakawa M, Kim S W, Hirano M, et al. Superconductivity in an inorganic electride 12CaO?7Al2O3:e-. J. Am. Chem. Soc., 2007,129(23): 7270-7271.
[12] Song C F, Sun J Q, Qiu S B, et al. Atomic fluorine anion storage emission material C12A7-F- and etching of Si and SiO2 by atomic fluorine anions. Chem. Mater., 2008, 20(10): 3473-3479.
[13] Sun J Q, Song C F, Ning S, et al. Preparation and characterization of storage and emission functional material of chlorine anion: [Ca24Al28O64]4+? (Cl-)3.80(O2-)0.10. Chin. J. Chem. Phys., 2009, 22(4): 417-422.
[14] Singh V K, Ali M M, Mandal U K. Formation kinetics of calcium aluminates. J. Am. Ceram. Soc., 1990, 73(4): 872-876.
[15] 朱英莲, 李远钊, 张培正, 等. 温度生长预测模型在大肠杆菌O157: H7控制中的应用. 食品科学, 2007, 28(3): 183-187.
[16] Yamamoto O. Influence of particle size on the antibacterial activity of zinc oxide. Int. J. Inorg. Mater., 2001, 3(7): 643-646.
[17] Sawai J, Shoji S, Igarashi H, et al. Hydrogen peroxide as an antibacterial factor in zinc oxide powder slurry. J. Ferment. Bioeng., 1998, 86(5): 521-522.
[18] Mendonca A F, Amoroso T L, Knabel S J. Destruction of gram-negative food-borne pathogens by high ph involves disruption of the cytoplasmic membrane. Appl. Environ. Microbiol., 1994, 60(11): 4009-4014.
[19] Sawai J, Kojima H, Igarashi H, et al. Escherichia coli damage by ceramic powder slurries. J. Chem. Eng. Jpn., 1997, 30(6): 1034-1039.
[20] Stoimenov P K, Klinger R L, Marchin G L, et al. Metal oxide nanoparticles as bactericidal agents. Langmuir, 2002, 18(17): 6679-6686.
[21] McCord J M. The evolution of free radicals and oxidative stress. American Journal of Medicine, 2000, 108(8): 652-659.
[22] Henchman M, Hierl P M, Paulson J F. Nucleophilic displacement vs proton transfer: the system OH-?(H2O)0,1,2+CH3Cl in the relative energy range 0.03-5eV. J. Am. Chem. Soc., 1985, 107(9): 2812-2814.
[23] Gao A M, Zhu X F, Wang H J, et al. Reduction features of NO over a potassium-doped C12A7-O- catalyst. J. Phys. Chem. B, 2006, 110 (24): 11854-11862.
[24] Lee J, Grabowski J J. Reactions of the atomic oxygen radical-anion and the synthesis of organic reactive Intermediates. Chemical Reviews, 1992, 92(7): 1611-1647.
[25] Dong T, Li J, Huang F, et al. One-step synthesis of phenol by O- and OH- emission material. Chem. Commun., 2005(21): 2724-2726.
[26] Wang Z X, Pan Y, Dong T, et al. Production of hydrogen from catalytic steam reforming of bio-oil using C12A7-O--based catalysts. Appl. Catal. A-Gen., 2007, 320: 24-34.
[27] Zhao E, Wang L, Yan L F, et al. Surface modification of medical poly(vinyl chloride) with O- water. J. Appl. Polym. Sci., 2008, 110(1): 39-48.

C12A7-O^-材料抗菌性能及机理研究

Investigation on the Antibacterial Activity and Mechanism of C12A7-O^- Particles

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 14

编辑推荐

Metrics

本文评价

[1]	盛丽丽, 常江. 光/磁热Fe₂SiO₄/Fe₃O₄双相生物陶瓷及其复合电纺丝膜制备及抗菌性能研究[J]. 无机材料学报, 2022, 37(9): 983-990.
[2]	王恩典, 常江. 钼掺杂铜硅钙石的制备及其抗菌性能和细胞相容性研究[J]. 无机材料学报, 2021, 36(7): 738-744.
[3]	刘玉玲, 王瑞莉, 李楠, 刘梅, 张青红. ZnO介晶填料的制备及其齿科复合树脂的性能[J]. 无机材料学报, 2019, 34(10): 1077-1084.
[4]	付海丽, 张雯, 张华, 宋少波, 李伟. 壳聚糖/有机锂皂石纳米复合材料的制备及抗菌性能研究[J]. 无机材料学报, 2016, 31(5): 479-484.
[5]	谭英, 谭帼馨, 宁成云, 容锡沧, 张余, 周蕾. 聚多巴胺修饰钛表面纳米载银及其抗菌和细胞相容性[J]. 无机材料学报, 2014, 29(12): 1320-1326.
[6]	张翔宇, 蒋立, 黄晓波, 马永, 范爱兰, 唐宾. 不锈钢表面渗铜扩散复合处理合金层的抗菌性能研究[J]. 无机材料学报, 2012, 27(5): 519-523.
[7]	李丹, 陈运法. 离子液体/磷酸锆抗菌材料的制备及耐热性研究[J]. 无机材料学报, 2011, 26(11): 1193-1198.
[8]	陈君华,王飞,程年寿,丁志杰,伏再辉,银董红. 铜原位改性HMS材料的表征及抗菌性能[J]. 无机材料学报, 2009, 24(4): 695-701.
[9]	戴晋明,侯文生,魏丽乔,贾虎生. 载银锌4A沸石抗菌剂抗变色性能的研究[J]. 无机材料学报, 2008, 23(5): 1011-1015.
[10]	韩晓建,黄争鸣,何创龙,刘玲,董国华,吴庆生. 聚碳酸酯/TiO₂超细纤维的制备与表征[J]. 无机材料学报, 2007, 22(3): 407-412.
[11]	李吉东,李玉宝,王学江,杨维虎,周钢. 载铜锌纳米羟基磷灰石的抗菌性能及机理研究[J]. 无机材料学报, 2006, 21(1): 162-168.
[12]	侯文生,魏丽乔,戴晋明,贾虎生,许并社. 载银4A沸石抗菌剂的制备及其抗菌性能的研究[J]. 无机材料学报, 2005, 20(4): 907-913.
[13]	叶瑛,周玉航,夏枚生,胡彩虹,凌洪飞. 新型无机抗菌材料:载铜蒙脱石及其抗菌机理讨论[J]. 无机材料学报, 2003, 18(3): 569-574.
[14]	柳清菊,王毓德,赵景畅,项金钟,吴兴惠. 强力安全的无机抗菌功能材料[J]. 无机材料学报, 2002, 17(1): 73-78.

C12A7-O-材料抗菌性能及机理研究

Investigation on the Antibacterial Activity and Mechanism of C12A7-O- Particles

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 14

编辑推荐

Metrics

本文评价

C12A7-O^-材料抗菌性能及机理研究

Investigation on the Antibacterial Activity and Mechanism of C12A7-O^- Particles