尿液中不同尺寸微晶的聚集过程

doi:10.3724/SP.J.1077.2012.00343

无机材料学报 ›› 2012, Vol. 27 ›› Issue (4): 343-347.DOI: 10.3724/SP.J.1077.2012.00343 CSTR: 32189.14.SP.J.1077.2012.00343

尿液中不同尺寸微晶的聚集过程

姚秀琼, 何节玉, 杨锦, 侯善华, 欧阳健明

(暨南大学生物矿化与结石病防治研究所, 广州 510632)

收稿日期:2011-04-29 修回日期:2011-09-05 出版日期:2012-04-10 网络出版日期:2012-03-12
作者简介:姚秀琼(1978-), 女, 博士研究生. E-mail: yaoxiuqiong-2006@163.com
基金资助:
广东省科技攻关项目(2009B030801236);国家自然科学基金(81170649)

Aggregation of Urinary Microcrystallines with Different Sizes

YAO Xiu-Qiong, HE Jie-Yu, YANG Jin, HOU Shan-Hua, OUYANG Jian-Ming

(Institute of Biomineralization and Lithiasis Research, Jinan University, Guangzhou 510632, China)

Received:2011-04-29 Revised:2011-09-05 Published:2012-04-10 Online:2012-03-12
About author:YAO Xiu-Qiong. E-mail: yaoxiuqiong-2006@163.com
Supported by:
Guangdong Technical Research Projects (2009B030801236);National Natural Science Foundation of China (81170649)

摘要/Abstract

摘要：

关键词: 草酸钙, 纳米晶, 快速团聚, 动力学

Abstract:

Key words: calcium oxalate, nanocrystal, rapid agglomeration, dynamics

中图分类号:

O614

姚秀琼, 何节玉, 杨锦, 侯善华, 欧阳健明. 尿液中不同尺寸微晶的聚集过程[J]. 无机材料学报, 2012, 27(4): 343-347.

YAO Xiu-Qiong, HE Jie-Yu, YANG Jin, HOU Shan-Hua, OUYANG Jian-Ming. Aggregation of Urinary Microcrystallines with Different Sizes[J]. Journal of Inorganic Materials, 2012, 27(4): 343-347.

图/表 4

参考文献 20

[1]	Fazil Marickar Y M, Salim A. Clinical risk index in urolithiasis. Urol. Res., 2009, 37: 283-287.
[2]	Laube N, Mohr, B, Hesse A. Laser-probe-based investigation of the evolution of particle size distributions of calcium oxalate particles formed in artificial urines. J. Crystal Growth, 2001, 233: 367-374.
[3]	He J Y, Deng S P, Ouyang J M. Morphology, size distribution, aggregation, and crystal phase of nanocrystallites in urines of healthy persons and lithogenic patients. IEEE Trans. Nanobiosci., 2010, 9(2): 156-163.
[4]	欧阳健明, 姚秀琼, 钟玖平, 等(OUYANG Jian-Ming, et al). 不同模拟体系中草酸钙结晶的比较研究. 高等学校化学学报(Chem. J. Chinese U.), 2002, 23(12): 2237-2239.
[5]	YANG Jin, LI Jun-Jun, YUAN Huan-Xin, et al. Regulation of potassium aminocarboxylates on calcium oxalate crystal growth and its relationship with molecular structure. Journal of Inorganic Materials, 2010, 25(11): 1185-1190.
[6]	LIU Yi-Ming, YUAN Huan-Xin, OUYANG Jian-Ming. Modulation of mixed potassium carboxylates on crystallization of calcium oxalate crystals in gel system. Journal of Inorganic Materials, 2007, 22(1): 75-78.
[7]	He J Y, Ouyang J M, Yang Y E. Agglomeration of urinary nanocrystallites: key factor to formation of urinary stone. Mater. Sci. Eng. C-Bio S, 2010, 30: 878-885.
[8]	柳一鸣, 何节玉, 邝荔, 等(LIU Yi-Ming, et al). 尿液中纳米微晶的团聚促进尿石的形成. 高等学校化学学报(Chem. J. Chinese U.), 2010, 31(5): 885-891.
[9]	Zetasizer Nano Series. User Manual. Malvern instruments, Ltd., UK. 2007: 2.
[10]	Volcke C, Pirotton S, Grandfils C, et al. Influence of DNA condensation state on transfection efficiency in DNA/polymer complexes: an AFM and DLS comparative study. J. Biotechnol,2006, 25(1): 11-21.
[11]	Finlayson B, Reid F. The expectation of free and fixed particles in urinary stone disease. Invest. Urol., 1978, 15(6): 442-448.
[12]	Hess B, Zipperle L, Jaeger P. Citrate and calcium effects on Tamm-Horsfall glycoprotein as a modifier of calcium oxalate crystal aggregation. Am. J. Physiol., 1993, 265(6): F784-F791.
[13]	Garten V A, Head R B. Homogeneous nucleation and phenomenon of crystalloluminescence. Phil. Mag., 1966, 14(132): 1243-1253.
[14]	居正华, 张旭, 王少刚, 等. TH蛋白和枸橼酸对尿草酸钙结晶动力学的影响的研究. 中国现代医学杂志, 2005, 15(10): 1496-1499.
[15]	Tsuji H, Tohru U, Hirotsugu U, et al. Urinary concentration of osteopontin and association with urinary supersaturation and crystal formation. Int. J. Urol., 2007, 14(7): 630-634.
[16]	Curreri P, Onoda G Y, Finlayson B. An electrophoretic study of calcium oxalate monohydrate. J. Colloid Interface Sci., 1979, 69(1): 170-182.
[17]	Kok D J, Papapoulos S E, Bijvoet O L. Crystal agglomeration is a major element in calcium oxalate urinary stone formation. Kidney Int., 1990, 37(1): 51-56.
[18]	Mandel N S, Mandel G S. Physicochemistry of Urinary Stone Formation. In: Renal Stone Disease. (Pak CYC, ed.) Martinus Nijhoff, Boston, MA, 1987: 1-24.
[19]	Burns J R, Finlayson B, Gauthier J. Calcium oxalate retention in subjects with crystalluria. Urol. Int., 1984, 39(1): 36-39.
[20]	Blomen L J M J. Growth and Agglomeration of Calcium Oxalate Crystals. University of Leiden, 1982.

	Membrane pore size /μm	Placing time, t / h					Δ */ nm
	Membrane pore size /μm	0	0.5	1	2	4	Δ */ nm
	0.22	167±80	533±144	854±193	1041±211	1606±281	1439
Stone formers	0.45	295±115	731±170	1124±224	1386±335	1852±373	1557
	1.2	772±210	1392±311	1831±310	1910±296	2306±379	1534
	3	1718±355	2060±259	2337±331	2509±390	2769±394	1051
	10	3251±531	2267±642	1657±261	3140±279	1550±352	?
	0.22	194±69	324±123	499±198	423±141	668±228	474
Healthy subjects	0.45	375±132	503±181	596±149	689±167	886±193	511
	1.2	457±143	619±156	690±201	806±194	1024±218	567
	3	615±213	655±187	731±146	921±215	1048±282	433
	10	721±231	751±251	838±213	959±311	1100±267	379

	Membrane pore size /μm	Placing time, t / h					Δ */ nm
	Membrane pore size /μm	0	0.5	1	2	4	Δ */ nm
	0.22	167±80	533±144	854±193	1041±211	1606±281	1439
Stone formers	0.45	295±115	731±170	1124±224	1386±335	1852±373	1557
	1.2	772±210	1392±311	1831±310	1910±296	2306±379	1534
	3	1718±355	2060±259	2337±331	2509±390	2769±394	1051
	10	3251±531	2267±642	1657±261	3140±279	1550±352	?
	0.22	194±69	324±123	499±198	423±141	668±228	474
Healthy subjects	0.45	375±132	503±181	596±149	689±167	886±193	511
	1.2	457±143	619±156	690±201	806±194	1024±218	567
	3	615±213	655±187	731±146	921±215	1048±282	433
	10	721±231	751±251	838±213	959±311	1100±267	379

	Instruments / μm	Placement time / h
	Instruments / μm	0	0.5	1	2	4
Stone former	NSA results / nm	295±115	731±170	1124±224	1386±335	1852±373
Stone former	TEM results / nm	275		1050		1600
Healthy subject	NSA results / nm	375±132	503±181	596±149	689±167	886±193
Healthy subject	TEM results / nm	170		300		600

	Instruments / μm	Placement time / h
	Instruments / μm	0	0.5	1	2	4
Stone former	NSA results / nm	295±115	731±170	1124±224	1386±335	1852±373
Stone former	TEM results / nm	275		1050		1600
Healthy subject	NSA results / nm	375±132	503±181	596±149	689±167	886±193
Healthy subject	TEM results / nm	170		300		600

尿液中不同尺寸微晶的聚集过程

Aggregation of Urinary Microcrystallines with Different Sizes

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