pH响应铜掺杂介孔硅纳米催化剂增强肿瘤化疗-化学动力学联合治疗的研究

doi:10.15541/jim20230151

无机材料学报

pH响应铜掺杂介孔硅纳米催化剂增强肿瘤化疗-化学动力学联合治疗的研究

何倩, 唐婉兰, 韩秉锟, 魏佳元, 吕文轩, 唐昭敏

西南石油大学新能源与材料学院, 成都 610500

收稿日期:2023-03-23 修回日期:2023-04-24 出版日期:2023-09-12 网络出版日期:2023-09-12
作者简介:何倩(1999-), 女, 硕士研究生. E-mail: 1084518887@qq.com
基金资助:
国家自然科学基金(51803174); 四川省玄武岩纤维复合材料开发及应用工程技术研究中心开放基金(2022SCXWYXWFC002)

pH Responsive Copper-Doped Mesoporous Silica Nanocatalyst for Enhanced Chemo-Chemodynamic Tumor Therapy

HE Qian, TANG Wanlan, HAN Bingkun, WEI Jiayuan, LV Wenxuan, TANG Zhaomin

School of New Energy and Materials, Southwest Petroleum University, Chengdu 610500, China

Received:2023-03-23 Revised:2023-04-24 Published:2023-09-12 Online:2023-09-12
About author:HE Qian (1999-), female, Master candidate. E-mail: 1084518887@qq.com
Supported by:
National Natural Science Foundation of China (51803174); Foundation of Sichuan Engineering Technology Research Center of Basalt Fiber Composite Development and Application of Southwest Petroleum University (2022SCXWYXWFC002)

摘要/Abstract

摘要： 化学动力学疗法(CDT)利用肿瘤细胞内源性H₂O₂与芬顿催化剂反应生成高毒性的羟基自由基(∙OH),从而杀死肿瘤细胞,但内源性H₂O₂不足和纳米粒子转运效率较低导致抗癌效果不理想。本研究制备了一种分散性良好、尺寸较小的铜掺杂介孔二氧化硅(Cu-MSN),负载化疗药物阿霉素(DOX)和抗坏血酸盐(AA)后,表面经叶酸(FA)和二甲基马来酸酐(DMMA)改性的壳聚糖(FA-CS-DMMA)以及羧甲基壳聚糖(CMC)包裹,得到pH响应型靶向纳米催化剂FA-CS-DMMA/CMC@Cu-MSN@DOX/AA (缩写为FCDC@Cu-MSN@DA)。扫描电镜显示纳米粒子FCDC@Cu-MSN@DA粒径约为100 nm。体外48 h内Cu²⁺释放量可达80%,药物DOX释放达到57.3%。释放的AA经自氧化后产生H₂O₂,诱导Cu²⁺发生类芬顿反应从而增强CDT。细胞实验证明FCDC@Cu-MSN@DA联合化疗药物表现出优异的抗肿瘤活性。该多功能纳米催化剂在癌症治疗中具有潜在的应用前景。

关键词: 癌症治疗, 铜离子, 过氧化氢, 纳米催化剂, 化学动力学疗法

Abstract: Chemodynamic therapy (CDT) uses endogenous H₂O₂ of tumor cells to react with Fenton catalysts to generate highly toxic hydroxyl radical (∙OH), thereby killing cancer cells. However, the insufficient endogenous H₂O₂ and low transport efficiency of nanoparticles result in unsatisfactory anticancer efficacy. In this work, we successfully synthesized a Cu²⁺ doped mesoporous silica nanoparticles (Cu-MSN) with excellent dispersity. After loaded with doxorubicin (DOX) and ascorbate (AA), Cu-MSN was coated with folic acid (FA), dimethyl maleic anhydride (DMMA) modified chitosan (FA-CS-DMMA) and carboxymethyl chitosan (CMC) to obtain a pH responsive targeted nanocatalyst FCDC@Cu-MSN@DA. SEM images showed that particle size of FCDC@Cu-MSN@DA was about 100 nm. After 48 h, cumulative amount of Cu²⁺release reached 80% and DOX release was about 57.3% in the acidic environment. After oxidation of AA, the produced exogenous H₂O₂ induced Cu²⁺to catalytic the Fenton-like reaction, which enhanced the therapeutic effect of tumor chemodynamic therapy (CDT). Cell viability in vitro demonstrated that FCDC@Cu-MSN@DA exhibited excellent anticancer ability in the combination of CDT and chemotherapy. All above data suggested that this multifunctional nanocatalyst has great potential application in cancer therapy in the future.

Key words: tumor therapy, copper iron, hydrogen peroxide, nanocatalyst, chemodynamic therapy

中图分类号:

TQ174

何倩, 唐婉兰, 韩秉锟, 魏佳元, 吕文轩, 唐昭敏. pH响应铜掺杂介孔硅纳米催化剂增强肿瘤化疗-化学动力学联合治疗的研究[J]. 无机材料学报, DOI: 10.15541/jim20230151.

HE Qian, TANG Wanlan, HAN Bingkun, WEI Jiayuan, LV Wenxuan, TANG Zhaomin. pH Responsive Copper-Doped Mesoporous Silica Nanocatalyst for Enhanced Chemo-Chemodynamic Tumor Therapy[J]. Journal of Inorganic Materials, DOI: 10.15541/jim20230151.

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pH响应铜掺杂介孔硅纳米催化剂增强肿瘤化疗-化学动力学联合治疗的研究

pH Responsive Copper-Doped Mesoporous Silica Nanocatalyst for Enhanced Chemo-Chemodynamic Tumor Therapy

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