基于介孔硼硅酸盐生物活性玻璃微球的可注射复合骨水泥

doi:10.15541/jim20200140

无机材料学报 ›› 2020, Vol. 35 ›› Issue (12): 1398-1406.DOI: 10.15541/jim20200140 CSTR: 32189.14.10.15541/jim20200140

所属专题：生物材料论文精选(2020)

• 研究快报 • 上一篇

基于介孔硼硅酸盐生物活性玻璃微球的可注射复合骨水泥

常宇辰¹(),林子扬¹,谢昕¹,吴章凡¹,姚爱华¹,叶松¹,林健¹,王德平¹(),崔旭²()

1. 同济大学材料科学与工程学院, 上海 201804
2. 中国科学院深圳先进技术研究院生物医药与技术研究所人体组织与器官退行性研究中心, 深圳 518055

收稿日期:2020-03-17 出版日期:2020-12-20 网络出版日期:2020-11-23
作者简介:常宇辰(1995–), 女, 硕士研究生. E-mail: changyuchencccc@163.com

An Injectable Composite Bone Cement Based on Mesoporous Borosilicate Bioactive Glass Spheres

CHANG Yuchen¹(),LIN Ziyang¹,XIE Xin¹,WU Zhangfan¹,YAO Aihua¹,YE Song¹,LIN Jian¹,WANG Deping¹(),CUI Xu²()

1. School of Materials Science and Engineering, Tongji University, Shanghai 201804, China
2. Center for Human Tissues and Organs Degeneration, Institute of Biomedicine and Biotechnolgy, Shenzhen Institutes of Advanced Technology, Chinese Academy of Science, Shenzhen 518055, China

Received:2020-03-17 Published:2020-12-20 Online:2020-11-23
About author:CHANG Yuchen(1995–), female, Master candidate. E-mail: changyuchencccc@163.com
Supported by:
National Key Research and Development Program of China(2018YFC1106302);National Natural Science Foundation of China(51772210);National Natural Science Foundation of China(51802340);National Natural Science Foundation of China(31870956);National Natural Science Foundation of China(81672227);Science and Technology Project of Guangdong Province-Doctoral Startup Fund of 2017(2017A030310318)

摘要/Abstract

摘要：

以溶胶-凝胶法制备的介孔硼硅酸盐生物活性玻璃微球(MBGS)作为固相, 海藻酸钠(SA)溶液作为液相,开发了一种可注射复合骨水泥。对MBGS中氧化硼/氧化硅的比例对其质构性能及骨水泥的可操作性、抗压强度和生物活性的影响进行表征。实验结果表明, 随着硼含量的增加, MBGS的比表面积从161.71 m²/g增大至214.28 m²/g, 平均孔径以及总孔容也随之增长, 加速了玻璃相中钙离子的释放, 使得玻璃与SA的快速交联, 改善了骨水泥可操作性能和力学性能, 凝固时间由21 min缩短至9 min, 抗压强度由3.4 MPa提升至4.1 MPa, 体外矿化性能也随之提高。综合各方面性能表现, BC-30骨水泥兼具良好的可操作性能、力学性能和体外矿化能力, 是最合适的骨水泥组分。总之, 提高MBGS的质构性能是增强复合骨水泥的可操作性、抗压强度和生物活性的有效方法。

关键词: 可注射骨水泥, 介孔硼硅酸盐生物活性玻璃, 生物活性, 可操作性

Abstract:

An injectable composite bone cement was fabricated by employing Sol-Gel derived mesoporous borosilicate bioactive glass spheres (MBGS) as solid phase and sodium alginate (SA) solution as liquid phase. The effects of the B₂O₃/SiO₂ ratio in MBGS on its textural properties and workability, compressive strength and bioactivity of bone cement were characterized. It is found that with the increase of boron content, the specific surface area of MBGS increases from 161.71 m²/g to 214.28 m²/g, and the average pore size and total pore volume also increase, which accelerates the Ca²⁺ release and rapidly crosslinks MBGS and SA. Thus, the workability, mechanical property and in vitro mineralization of bone cement are significantly improved, as the setting time is shortened from 21 min to 9 min and the compressive strength is enhanced from 3.4 MPa to 4.1 MPa. Comprehensive performance of all aspects, BC-30 bone cement showing good workability, mechanical properties and in vitro mineralization ability, displays the optimal component. Therefore, improving the textural properties of MBGS is an effective method to enhance the workability, compressive strength and bioactivity of composite bone cement.

Key words: injectable bone cement, mesoporous borosilicate bioactive glass, bioactivity, workability

中图分类号:

TQ171

常宇辰, 林子扬, 谢昕, 吴章凡, 姚爱华, 叶松, 林健, 王德平, 崔旭. 基于介孔硼硅酸盐生物活性玻璃微球的可注射复合骨水泥[J]. 无机材料学报, 2020, 35(12): 1398-1406.

CHANG Yuchen, LIN Ziyang, XIE Xin, WU Zhangfan, YAO Aihua, YE Song, LIN Jian, WANG Deping, CUI Xu. An Injectable Composite Bone Cement Based on Mesoporous Borosilicate Bioactive Glass Spheres[J]. Journal of Inorganic Materials, 2020, 35(12): 1398-1406.

图/表 14

Table 1 The compositions of MBGS/mol%

Glass	B₂O₃	SiO₂	CaO	P₂O₅
MBGS-20	20	40	36	4
MBGS-30	30	30	36	4
MBGS-40	40	20	36	4

Fig. 1 XRD patterns of the as-prepared MBGS

Fig. 2 TEM images of the as-prepared MBGS

Table 2 Specific surface area, average pore diameter and total pore volume of the three groups of MBGS

Sample	Specific surface area/(m²?g^-1)	Average pore diameter/nm	Total pore volume/(mL?g^-1)
MBGS-20	161.71	13.33	0.052
MBGS-30	176.98	13.97	0.057
MBGS-40	214.28	15.11	0.079

Fig. 3 FT-IR spectra of the as-prepared MBGS

Fig. 4 (a) N2 adsorption-desorption isotherms and (b) pore size distributions of MBGS-20, MBGS-30, and MBGS-40

Fig. 5 SEM images of (a, d) BC-20, (b, e) BC-30, and (c, f) BC-40 bone cement specimens

Fig. 6 (a) Setting time and (b) compressive strength of BC-20, BC-30, and BC-40, respectively * indicates significant difference between groups, p<0.05

Fig. 7 (a) Force-displacement curves and (b) injectabilities of mesoporous borosilicate bone cements for different B2O3 contents * indicates significant difference between groups, p<0.05

Fig. 8 Photographs of the mesoporous borosilicate bone cement extruded from the syring

Fig. 9 Anti-washout properties of mesoporous borosilicate bone cements

Fig. 10 Morphologies of mesoporous borosilicate bone cement specimens after being shaken in PBS at 37 ℃ for 2, 5, 12, and 24 h, respectively

Fig. 11 XRD patterns of bone cement specimens after being immersed in SBF for 3, 7 and 14 d, respectively

Fig. 12 SEM images and EDS data of (a, d) BC-20, (b, e) BC-30, (c, f) BC-30 after being immersed in SBF for 14 d

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基于介孔硼硅酸盐生物活性玻璃微球的可注射复合骨水泥

An Injectable Composite Bone Cement Based on Mesoporous Borosilicate Bioactive Glass Spheres

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