Journal of Inorganic Materials ›› 2017, Vol. 32 ›› Issue (3): 319-325.doi: 10.15541/jim20160260

• Orginal Article • Previous Articles     Next Articles

Surface Microstructure on Hydroxyapatite Spherules and Its Regulation on Stem Cells

Wei ZHI1(), Feng SHI1, Jing-Yu LI1, Teng ZHOU1, Shu-Xin QU1, Jian-Xin WANG1, Cong ZHANG2, Jie WENG1()   

  1. 1. Key Laboratory of Advanced Technologies of Materials (Ministry of Education), School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China
    2. The 309st Hospital of Chinese PLA, Beijing 100091, China
  • Received:2016-04-18 Revised:2016-07-07 Online:2017-03-20 Published:2017-02-24
  • About author:ZHI Wei. E-mail:
  • Supported by:
    National Key Research and Development Program of China (2016YFC110200, 2016YFB07000800);National Natural Science Foundation of China (31400809);Fundamental Research Funds for the Central Universities (2682015CX006)


HA spheres with different surface microstructures were prepared by a Sol-Gel route with various hydroxyapatite (HA)/chitin ratios. The surface morphologies, phase compositions, and biomimetic mineralization ability of samples were respectively analyzed with scanning electron microscopy (SEM), X-ray diffraction (XRD), and biomimetic mineralization test. Furthermore, the influences of the surface microstructure on biological behavior of bone marrow-derived mesenchymal stem cells were examined by Alamar blue, SEM, alkaline phosphatase assay and flow cytometry. When the mass ratio of HA/Chitin was increased from 4/1 to 35/1, the surface microstructure of HA spheres changed substantially, as showed by a reduction of micro-porosity from (35%±0.8%) to (10.4%±0.7%), decrease of surface roughness, and gradual disappearance of micro-creases. The result of biomimetic mineralization test showed that the surface microstructure had important influence to the ability of biomimetic mineralization for HA spheres. In vitro culture of bone marrow-derived mesenchymal stem cells demonstrated that the rougher sphere surface, with abundant micro-creases and micro-pores, supported cell spreading and proliferation. In comparison, the smoother sphere surface with fewer micro-pores effectively induced cell elongation and up-regulated ALP expression, which suggested stem cells osteogenic differentiation. Meanwhile, the surface microstructure of spherules also modulated the expression of characteristic antigen makers on the surface of stem cells.

Key words: hydroxyapatite, surface microstructure, stem cell, biological behavior

CLC Number: 

  • R318