Study on Hemostatic Materials of Mesoporous Silicon Dioxide Doped Ca and Ag with Antibacterial Properties

doi:10.3724/SP.J.1077.2012.00513

Abstract

Abstract:

An antibacterial hemostatic material of mesoporous silicon oxide gel doped with Ca and Ag (m-SCA) gel was fabricated by using cetyltrimethylammonium bromide(CTAB) as templates and Sol-Gel process. The results showed that m-SCA with mesoporous structure had the surface area of 416 m²/g and pore size of around 2 nm. The m-SCA with high surface area and good clotting function could significantly shorten the prothrombin time (PT) and activated partial thromboplastin time (APTT). The m-SCA containing a small quantity of Ag had no obvious effects on its clotting properties while Ca in m-SCA could promote clotting. The m-SCA doped 0.02wt% Ag without cytotoxicity had good antibacterial properties against E.coli. Animal experimental results showed that the m-SCA could control hemorrhage and shorten the bleeding time of rabbit ear wound, showing good hemostatic properties. It suggestes that the m-SCA is a good hemostatic material with antibacterial properties.

Key words: Ag-doped, Ca-doped, mesoporous silicon oxide gel, hemostatic materials, antibacterial, biocompatibility

CLC Number:

R318

CHEN Yu, LI Wen-Rui, XU Can, SU Jia-Can, LI Ming, LIU Chang-Sheng. Study on Hemostatic Materials of Mesoporous Silicon Dioxide Doped Ca and Ag with Antibacterial Properties[J]. Journal of Inorganic Materials, 2012, 27(5): 513-518.

Figures/Tables 9

Fig. 1 Micro-morphologies of mesoporous silicon dioxide gel doped with Ca and Ag (a,b) and without Ca and Ag(c,d)

Fig. 2 Nitrogen adsorption-desorption isoterms (a) and pore size distribution (b) of m-SCA (5wt% Ca, 0.02wt% Ag)

Fig. 3 Water adsorption properties of m-SCA (m-SA and SD as controls)

Fig. 4 m-SCA release Ca and Ag ions into PBS with time

Fig.5 Clotting time (PT and APTT) of m-SCA(m-SA and SD as controls)

Fig. 6 Effects of Ca content on clotting time (PT and APTT) of m-SCA

Fig. 7 Effects of Ag ontetent on antibacterial properties of m-SCA against E.coli incubation for 1h (a) and (b) 12 h

Fig. 8 Effects of m-SCA extracts on L929 cell proliferation(m-SC and SD as controls)

Fig. 9 Bleeding time of ear wound after being applied by m-SCA(m-SC and SD as controls)

References 19

[1]	Bjorsesa K, Holst J. Various local hemostatic agents with different modes of action; an in vivo comparative randomized vascular surgical experimental study. Eur. J. Vasc. Endovasc. , 2007, 33(3): 363-370.
[2]	Sohn V Y, Eckert M J, Martin M J, et al. Efficacy of three topical hemostatic agents applied by medics in a lethal groin injury model. J. Surg. Res. , 2009, 154(2): 258-261.
[3]	Humphreys M R, Castle E P,Andrews P E, et al. Microporous polysaccharide hemospheres for management of laparoscopic trocar injury to the spleen. Am. J. Sur., 2008, 195(1): 99-103.
[4]	Khalid AL-Qahtani. Initial experience with hemostatic fibrin glue as adjuvant during drainless parotidectomy. Saudi. Med. J. , 2011, 23(2): 67-71.
[5]	Hoffmann N E, Siddiqui S A, Agarwal S, et al. Choice of hemostatic agent influences adhesion formation in a rat cell adhesion model. J. Sur. Res., 2009, 155(1): 77-81.
[6]	Ong S Y, Wu J, Moochhala S M, et al. Development of a chitosan- based wound dressing with improved hemostatic and antimicrobial properties. Biomaterials, 2008, 29(32): 4323-4332.
[7]	Emily Blocha, Trang Phanb, Denis Bertinb, et al. Direct synthesis of mesoporous silica presenting large and tunable pores using BAB triblock copolymers: influence of each copolymer block on the porous structure. Micropor. Mesopor. Mat., 2007, 112(1/2/3): 612-620.
[8]	Dai Chenglong, Liu Changsheng, Wei Jie, et al. Molecular imprinted macroporous chitosan coated mesoporous silica xerogels for hemorrhage control. Biomaterials, 2010, 31(30):7620-7630.
[9]	LI Xiao-Sheng, LIU Chang-Sheng, YUAN Yuan, et al. Preparation and hemostatic properties of mesoporous silica-based xerogels. Journal of Inorganic Materials, 2008, 23(2): 327-331.
[10]	余承忠, 严晓霞, 赵东元. 新型的介孔分子筛止血材料及其制备方法. 中国发明专利, ZL200510026838. 9, 2006.02.01.
[11]	Hobday C D, Milam M R, Milam R A, et al. Postoperative small bowel obstruction associated with use of hemostatic agents. J. Minim. Invas. Gyn. , 2009, 16(2): 224-226.
[12]	Simon Silver. Bacterial silver resistance: molecular biology and uses and misuses of silver compounds. FEMS Microbiol. Rev., 2003, 27(2/3):341-353.
[13]	Percival S L, Bowler P G, Russell D. Bacterial resistance to silver in wound care. J. Hospl. Infect. , 2005, 60(1):1-7.
[14]	Dai Chenglong, Yuan Yuan, Liu Changsheng, et al. Degradable, antibacterial silver exchanged mesoporous silica spheres for hemorrhage control. Biomaterials, 2009, 30(29): 5364-5375.
[15]	Wei Jie, Chen Fangping, Shin Jung-Woog, et al. Preparation and chara- cte------rization of bioactive mesoporous wollastonit-polycaprolactone composite scaffold. Biomaterials, 2009, 30(6): 1080-1088.
[16]	Parveen Kumar, Vadim V Guliants. Periodic mesoporous organic-inorganic hybrid materials: applications in membrane separations and adsorption. Micropor. Mesopor. Mat., 2010, 132(1/2): 1-14.
[17]	Emily W Y Tam, Glenn Rosenbluth, Elizabeth E Rogers. Cerebellar hemorrhage on magnetic resonance imaging in preterm newborns associated with abnormal neurologic outcome. The Journal of Pediatrics, 2011, 158(2): 245-250.
[18]	Ostomel T A, Shi Q, Stoimenov P K, et al. Metal oxide surface charge mediated hemostasis. Langmuir, 2007, 23(22): 11233-11238.
[19]	Tuthill D D, Bayer V, Gallagher A M, et al. Assessment of topical hemostats in a renal hemorrhage model in heparinized rats. J. Surg. Res., 2001, 95(2): 126-132.