Fe3O4-DMSA-PEI Magnetic Nanoparticles with Small Particle Size: Preparation and Gene Loading

doi:10.15541/jim20230464

Abstract

Abstract:

Ferroferric oxide (Fe₃O₄) magnetic nanoparticles are widely used as passive targeting carriers in gene therapy, due to their simple preparation, targeting under external magnetic field and easy surface grafting. This study synthesized oil phase Fe₃O₄ nanoparticles with controllable particle sizes in the range from 4 to 9 nm by regulating the accumulation growth time in the solvothermal method. Then, meso-2, 3-dimercaptosuccinic (DMSA) was employed to double exchange oleic acid molecules on its surface to provide good water dispersibility. Finally, Fe₃O₄-DMSA-PEI magnetic nanoparticles were obtained by grafting branched polyethylenimine (PEI) onto Fe₃O₄-DMSA surface through amidization reaction. The results demonstrate that the Fe₃O₄-DMSA-PEI magnetic nanoparticles have a surface Zeta potential of (52.50 ± 1.94) mV, remaining a certain degree of superparamagnetism (14.48 emu/g, 1 emu/g=1 A∙m²/kg). When the mass ratio of Fe₃O₄-DMSA-PEI magnetic nanoparticles to plasmid DNA is 15 : 1, it can completely block DNA and its loading capacity is as high as 6.67%. The Fe₃O₄-DMSA-PEI magnetic nanoparticles prepared in this study have a certain gene delivery ability and are expected to be used as gene carriers in the field of gene transfection.

Key words: ferroferric oxide, magnetic nanoparticle, gene carrier, surface modification

CLC Number:

TB383

CAI Heqing, HAN Lu, YANG Songsong, XUE Xinyu, ZHANG Kou, SUN Zhicheng, LIU Ruping, HU Kun, WEI Yan. Fe₃O₄-DMSA-PEI Magnetic Nanoparticles with Small Particle Size: Preparation and Gene Loading[J]. Journal of Inorganic Materials, 2024, 39(5): 517-524.

Figures/Tables 10

Fig. 1 Route map of PEI grafting on the surface of Fe3O4 nanoparticles

Fig. 2 TEM images of Fe3O4-OA (a) MNP-1; (b) MNP-2; (c) MNP-3

Fig. 3 Particle size distributions (a, c) and polydispersity indexs (b, d) of samples (a, b) Fe3O4-OA (MNP-1, MNP-2 and MNP-3); (c, d) Fe3O4-OA, Fe3O4-DMSA and Fe3O4-DMSA-PEI Colorful figures are available on website

Fig. 4 FT-IR spectra of Fe3O4-OA, Fe3O4-DMSA and Fe3O4-DMSA-PEI

Fig. 5 Raman spectra of Fe3O4-OA and Fe3O4-DMSA

Fig. 6 XRD pattern of Fe3O4-DMSA-PEI

Fig. 7 Zeta potential histogram of Fe3O4-OA, Fe3O4-DMSA and Fe3O4-DMSA-PEI

Fig. 8 Variable temperature magnetic induction curves of (a) Fe3O4-OA, (b) Fe3O4-DMSA and (c, d) Fe3O4-DMSA-PEI

Fig. 9 Hysteresis loops of Fe3O4-OA, Fe3O4-DMSA and Fe3O4-DMSA-PEI 1 emu/g=1 A∙m2/kg; 1 Oe=79.6 A/m

Fig. 10 Gel electrophoresis image of Fe3O4-DMSA-PEI(1800) blocking plasmid DNA

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Fe₃O₄-DMSA-PEI Magnetic Nanoparticles with Small Particle Size: Preparation and Gene Loading

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