Mineralization Activity of Li2Ca2Si2O7 Bioceramics

doi:10.15541/jim20200657

Abstract

Abstract:

How to effectively treat periodontitis and achieve regeneration of damaged periodontal tissue has always been a challenging problem in the treatment of periodontal diseases, and mineralization is one of the key factors in the normal periodontal development and function. The aim of this study was to investigate the effects of Li₂Ca₂Si₂O₇ bioceramics on proliferation and mineralization of human periodontal ligament fibroblasts (HPLFs) and possibility of its application in periodontal tissue regeneration. Li₂Ca₂Si₂O₇ ceramic powder was synthesized by Sol-Gel method. It was found that Li₂Ca₂Si₂O₇ powder had good ability of hydroxyapatite mineralization by immersing in simulated body fluid. The in vitro biological results showed that Li₂Ca₂Si₂O₇powder extract could significantly promote proliferation of HPLFs in the concentration range of 3.125~25 mg/mL, and at low concentration (6.25 mg/mL), could significantly induce mineralization of HPLFs cells (p<0.05). All these experimental results reveal that the new Li₂Ca₂Si₂O₇powder can promote proliferation and mineralization of HPLFs, and it may be a promising bioactive material for periodontal tissue regeneration.

Key words: Li₂Ca₂Si₂O₇bioceramic, human periodontal ligament fibroblast, proliferation, mineralization

CLC Number:

TQ174

XU Hongyi, ZHAI Dong, CAO Wanting, CHEN Zhenhua, QIAN Wenhao, CHEN Lei. Mineralization Activity of Li₂Ca₂Si₂O₇ Bioceramics[J]. Journal of Inorganic Materials, 2021, 36(7): 753-760.

Figures/Tables 8

Fig. 1 XRD pattern (A), SEM morphology (B) and particle size distribution (C) of Li2Ca2Si2O7 powders

Fig. 2 XRD patterns of Li2Ca2Si2O7 powders soaked in SBF for different periods

Fig. 3 SEM images of Li2Ca2Si2O7 powders soaked in SBF for different periods (A) 0 d; (B) 1 d; (C) 3 d; (D) 7 d; (E) 14 d; (F) 14 d (enlarge image)

Fig. 4 Ions concentrations of Li2Ca2Si2O7 powders immersed in SBF for different periods

Fig. 5 Proliferation of HPLFs induced by Li2Ca2Si2O7 powders extract (* p<0.05, ** p<0.01, *** p<0.001)

Table 1 Ion concentrations of Li2Ca2Si2O7 powders extracts

Ionic concentration/(mg·L^-1)	Powder extract concentrations /(mg·mL^-1)
Ionic concentration/(mg·L^-1)	Control	3.125	6.25	12.5	25	50	100	200
Li	0	13.45	27.31	53.42	114.31	225.79	440.40	864.14
Ca	69.46	68.61	68.91	65.46	60.36	56.82	46.04	23.16
Si	1.19	13.62	26.16	51.57	102.70	205.01	416.02	840.13
P	28.37	27.48	26.44	25.28	22.19	19.66	13.94	0.71

Fig. 6 Cytoskeleton staining of HPLFs induced by Li2Ca2Si2O7 powders extract (A-C) Blank control; (D-F) 6.25 mg/mL; (G-I) 50 mg/mL; Blue: Nucleus; Red: Cytoskeleton

Fig. 7 Alizarin red staining of HPLFs induced by Li2Ca2Si2O7 powders extract (A) Blank control; (B) 6.25 mg/mL; (C) 50 mg/mL; (D) Mineralization. *: p<0.05; ***: p<0.001

References 34

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Mineralization Activity of Li₂Ca₂Si₂O₇ Bioceramics

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