Effect of Silk Fibroin Content on the Bionic Mineralization and In Vitro Cellular Compatibility of Silk Fibroin/Hydroxyapaptite Nanocomposites

doi:10.3724/SP.J.1077.2012.11536

Abstract

Abstract: Silk fibroin and hydroxyapatite nanocomposites (SF/HA) with various SF content were prepared. Effect of Silk fibroin content on the bionic mineralization and cellular compatibility in vitro of HA nanocrystals (n-HA) was investigated. The results show that SF content has an obvious effect on the nucleation and growth of n-HA. However, though SF content does not show obvious difference on the nucleation and growth of n-HA, it has obvious effect on the n-HA aggregation. When SF content is less than 20wt%, n-HA orderly disperses in SF matrix. While the SF content is more than 20wt%, the n-HA aggregation becomes disordered. The in vitro cellular compatibility experiments demonstrate that the SF/HA composites exhibit better cell affinity than pure n-HA. However, SF content has no obvious effect on the cell affinity of n-SF/HA 20wt% SF/HA and 30wt% SF/HA show better osteoblast proliferation.

Key words: hydroxyapaptite, silk fibrion content, nanocrystals, bionic mineralization, cytocompatibility

CLC Number:

TB321

ZHU Yun-Rong, CHEN Yu-Yun, XU Guo-Hua, YE Xiao-Jian, ZHONG Jian, HE Dan-Nong. Effect of Silk Fibroin Content on the Bionic Mineralization and In Vitro Cellular Compatibility of Silk Fibroin/Hydroxyapaptite Nanocomposites[J]. Journal of Inorganic Materials, 2012, 27(8): 883-886.

References

[1] WangYongzhong, Kim Hyeon-Joo, Gordana Vunjak-Novakovic, et al. Stem cell-based tissue engineering with silk biomaterials. Biomaterials, 2006, 27(36): 6064-6082.

[2] Kong X D, Cui F Z, Wan X M, et al. Silk -broin regulated mineralization of hydroxyapatite nanocrystals. Journal of Crystal Growth, 2004, 270(1/2): 197-202.

[3] 王刚, 姚金波, 周旭光, 等. “一步法” 制备纳米相丝素蛋白/羟基磷灰石生物复合材料. 复合材料学报, 2008, 25(6): 136-139.

[4] WANG Jiang, ZUO Yi, YANG Wei-Hu, et al. Study on preparation of n-HA and silk fibroin bio-mineral material. Journal of Inorganic Materials, 2009, 24(2): 264-268.

[5] Wang Li Ning,Gui-Ling Senna Mamoru. Microstructure and gelation behavior of hydroxyapatite-based nanocomposite sol containing chemically modi-ed silk -broin. Colloids and Surfaces A, 2005, 254 (1/2/3): 159-164.

[6] Kong Xiangdong, Sun Xiaodan, Cui Fuzhai, et al. Effect of solute concentration on fibroin regulated biomineralization of calcium phosphate. Materials Science and Engineering C, 2006, 26(4): 639-643.

[7] Sun Xiao-Dan, Zhou Ying-Li, Ren Juan-Yong, et al. Effect of pH on the -broin regulated mineralization of calcium phosphate. Current Applied Physics, 2007, 7(S1): 75-79.

[8] 王江, 杨维虎, 沈宝珍, 等. 纳米羟基磷灰石/丝素蛋白生物复合材料的制备和表征. 高分子材料科学与工程, 2008, 24(11): 165-168.

[9] 杨辉, 张林, 张宏, 等. 丝素蛋白/羟基磷灰石复合材料的制备及性能表征. 复合材料学报, 2007, 24(3): 141-146.

[10] LI Zhi-Hong, WU Ji-Min, HUANG Shu-Jie et al. Effect of pretreatment on fabrication of natural fibroin fiber/apatite composites using alternate soaking method. Journal of Inorganic Materials, 2011, 26(1): 43-48.

[11] 曹惠, 陈新, 邵正中(CAO Hui, et al). 羟基磷灰石/丝素蛋白复合纤维的制备及其矿化研究. 化学学报(Acta Chim. Sinica), 2008, 66(18): 2059-2064.

[12] Li Yucheng, Cai Yurong, Kong Xiangdong, et al. Anisotropic growth of hydroxyapatite on the silk -broin -lms. Applied Surface Science, 2008, 255(5): 1681-1685.

[13] Du Chunling, Jin Jun, Li Yucheng, et al. Novel silk -broin/hydroxyapatite composite -lms: structure and properties. Materials Science and Engineering C, 2009, 29(1): 62-68.

[14] 刘琳, 孔祥东, 李玉成, 等(LIU Lin, et al). 预处理对丝素蛋白膜调控羟基磷灰石晶体生长的影响. 高等学校化学学报(Chem. J. Chinese N.), 2009, 30(10): 1987-1991.

[15] 姚菊明, 魏克民, 励丽, 等(YAO Ju-Ming, et al). 蚕丝素蛋白初始结构对其矿化作用的影响. 化学学报(Acta Chim. Sinica), 2007, 65(7): 635-639.

[16] Fan Chuanquan, Li Jiashun, Xu Guohua, et al. Facile fabrication of nano-hydroxyapatite/silk fibroin composite via a simplified coprecipitation route. Journal of Material Science, 2010, 45(21): 5814-5819.

[17] Zhu Yunrong, Chen Yuyun, Xu Guohua, et al. Micropattern of nano-hydroxyapatite/silk fibroin composite onto Ti alloy surface via template-assisted electrostatic spray deposition. Materials Science and Engineering C, 2012, 32(2): 390-394.