丝素蛋白含量对纳米羟基磷灰石仿生矿化和体外细胞相容性的影响

doi:10.3724/SP.J.1077.2012.11536

无机材料学报 ›› 2012, Vol. 27 ›› Issue (8): 883-886.DOI: 10.3724/SP.J.1077.2012.11536 CSTR: 32189.14.SP.J.1077.2012.11536

丝素蛋白含量对纳米羟基磷灰石仿生矿化和体外细胞相容性的影响

朱云荣¹, 陈玉云², 许国华¹, 叶晓健¹, 钟健², 何丹农²

(1. 长征医院, 上海 200003; 2. 纳米技术及应用国家工程研究中心, 上海 200241)

收稿日期:2011-08-25 修回日期:2011-11-28 出版日期:2012-08-20 网络出版日期:2012-07-09
作者简介:朱云荣(1984-), 男, 博士研究生. E-mail: zyr13579@hotmail.com
基金资助:
国家重点基础研究发展计划(2009CB930000); 上海市自然科学基金(0852nm03100)

Effect of Silk Fibroin Content on the Bionic Mineralization and In Vitro Cellular Compatibility of Silk Fibroin/Hydroxyapaptite Nanocomposites

ZHU Yun-Rong¹, CHEN Yu-Yun², XU Guo-Hua¹, YE Xiao-Jian¹, ZHONG Jian², HE Dan-Nong²

(1. Changzheng Hospital, Shanghai 200003, China; 2. National Engineering Research Center for Nanotechnology, Shanghai 200241, China)

Received:2011-08-25 Revised:2011-11-28 Published:2012-08-20 Online:2012-07-09
About author:ZHU Yun-Rong. E-mail: zyr13579@hotmail.com
Supported by:
973 Program (2009CB930000); Natural Science Research Foundation of Shanghai (0852nm03100)

摘要/Abstract

摘要： 通过制备含有不同丝素蛋白(SF)含量的丝素蛋白/纳米羟基磷灰石复合材料, 着重研究丝素蛋白含量对纳米羟基磷灰石(n-HA)仿生矿化过程中晶体成核与生长及体外细胞相容性的影响. 结果表明: SF的加入对n-HA晶体的成核和生长具有明显的调控作用; 丝素含量的大小对n-HA晶体成核和生长没有明显的区别, 但对晶粒在有机大分子中的聚集状态有明显的影响: 当SF含量不超过20wt%时, n-HA晶粒呈现放射状团簇, 当SF含量超过20wt%时, n-HA晶粒无序团聚. 体外细胞相容性结果显示, SF的加入可以促进材料与细胞的界面亲和性, 但SF含量对这种亲和性的影响不明显, 20wt%和30wt%的SF含量对增殖能力具有较强的促进作用.

关键词: 羟基磷灰石, 丝素蛋白含量, 纳米晶, 仿生矿化, 细胞相容性

Abstract: Silk fibroin and hydroxyapatite nanocomposites (SF/HA) with various SF content were prepared. Effect of Silk fibroin content on the bionic mineralization and cellular compatibility in vitro of HA nanocrystals (n-HA) was investigated. The results show that SF content has an obvious effect on the nucleation and growth of n-HA. However, though SF content does not show obvious difference on the nucleation and growth of n-HA, it has obvious effect on the n-HA aggregation. When SF content is less than 20wt%, n-HA orderly disperses in SF matrix. While the SF content is more than 20wt%, the n-HA aggregation becomes disordered. The in vitro cellular compatibility experiments demonstrate that the SF/HA composites exhibit better cell affinity than pure n-HA. However, SF content has no obvious effect on the cell affinity of n-SF/HA 20wt% SF/HA and 30wt% SF/HA show better osteoblast proliferation.

Key words: hydroxyapaptite, silk fibrion content, nanocrystals, bionic mineralization, cytocompatibility

中图分类号:

TB321

朱云荣, 陈玉云, 许国华, 叶晓健, 钟健, 何丹农. 丝素蛋白含量对纳米羟基磷灰石仿生矿化和体外细胞相容性的影响[J]. 无机材料学报, 2012, 27(8): 883-886.

ZHU Yun-Rong, CHEN Yu-Yun, XU Guo-Hua, YE Xiao-Jian, ZHONG Jian, HE Dan-Nong. Effect of Silk Fibroin Content on the Bionic Mineralization and In Vitro Cellular Compatibility of Silk Fibroin/Hydroxyapaptite Nanocomposites[J]. Journal of Inorganic Materials, 2012, 27(8): 883-886.

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丝素蛋白含量对纳米羟基磷灰石仿生矿化和体外细胞相容性的影响

Effect of Silk Fibroin Content on the Bionic Mineralization and In Vitro Cellular Compatibility of Silk Fibroin/Hydroxyapaptite Nanocomposites

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