不同浓度硝酸处理的多孔钛及其体外生物活性

doi:10.3724/SP.J.1077.2012.11691-1

无机材料学报 ›› 2012, Vol. 27 ›› Issue (5): 555-560.DOI: 10.3724/SP.J.1077.2012.11691-1 CSTR: 32189.14.SP.J.1077.2012.11691-1

• • 上一篇

不同浓度硝酸处理的多孔钛及其体外生物活性

胥彬¹, 赵朝勇¹, 蔡兵², 范红松¹

(四川大学 1. 国家生物医学材料工程技术研究中心; 2. 分析测试中心, 成都 610064)

收稿日期:2011-11-10 出版日期:2012-05-10 网络出版日期:2012-03-31
作者简介:XU Bin(1987-), male, candidate of master degree. E-mail: xubin1987.happy@163.com

Porous Titanium Treated by Nitric Acid with Varied Concentration and the Bioactivity in Vitro

XU Bin¹, ZHAO Chao-Yong¹, CAI Bing², FAN Hong-Song¹

(1. National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, China; 2. Analytical and Testing Center, Sichuan University, Chengdu 610064, China)

Received:2011-11-10 Published:2012-05-10 Online:2012-03-31
About author:XU Bin(1987-), male, candidate of master degree. E-mail: xubin1987.happy@163.com
Supported by:
Foundation item: National Key Technologies R&D Program of China (2006BAI16B01);Key Technologies R&D Program of Sichuan Province, China (2010SZ0121)

摘要/Abstract

摘要：

多种表面活化改性方法已用于致密钛表面活化改性, 本研究尝试用不同浓度的硝酸溶液处理浆料发泡法制备的多孔钛. 酸处理多孔钛内孔壁上出现了微小尺寸的酸蚀坑, 其微结构随酸浓度的变化而略有不同, 处理后表面磷灰石沉积能力随酸浓度变化差异显著, 其中以1:1和1:5两种浓度(体积比)的硝酸处理组最强, 并显著促进MG63细胞增殖, 细胞在样品表面和孔内得到良好粘附和铺展. 本研究结果表明硝酸处理活化多孔钛, 方法简单, 不引入杂质, 渗透性好, 可有效活化多孔钛内外表面, 是一种有效的制备生物活性多孔钛的方法.

关键词: 多孔钛, 生物活性, 表面处理, 硝酸处理

Abstract:

Surface modification is expected to further activate the titanium implant and has been widely used in dense titanium surface. In this study, porous titanium fabricated by a slurry foaming method was treated by nitric acid solution with different concentrations. Micrometer-sized acid-etched pits were observed on the inner-pore walls of porous titanium and the morphology varied a little with nitric acid concentration. However, the ability to deposit apatite on the treated porous titanium surface changed obviously with nitric acid concentration. The samples treated by nitric acid solution at a ratio of 1:1 and 1:5 (V(HNO₃):V(H₂O)) showed the best apatite-forming ability. Compared with that non-treated porous titanium, the samples were treated by nitric acid at the ration of 1:1 and 1:5 (V(HNO₃):V(H₂O)) showed higher MG63s cells viability, better cells adhesion and spreading with cells both on the surface and inside the pores. The results showed that nitric acid treatment, a simple process with less impurity introduced and good penetration inside porous titanium was an effective method to prepare bioactive porous titanium.

Key words: porous titanium, bioactivity, surface modification, nitric acid treatment

中图分类号:

R783

胥彬, 赵朝勇, 蔡兵, 范红松. 不同浓度硝酸处理的多孔钛及其体外生物活性[J]. 无机材料学报, 2012, 27(5): 555-560.

XU Bin, ZHAO Chao-Yong, CAI Bing, FAN Hong-Song. Porous Titanium Treated by Nitric Acid with Varied Concentration and the Bioactivity in Vitro[J]. Journal of Inorganic Materials, 2012, 27(5): 555-560.

图/表 7

Fig. 1 FESEM images of porous titanium subjected to different concentrations of HNO3 aqueous solution treatment

Fig. 2 XRD patterns of porous titanium subjected to different concentrations of HNO3 aqueous solution

Fig. 3 FESEM photographs of porous titanium subjected to different concentrations of HNO3 aqueous solution treatment after immersion in SBF for 1 d (a, b) no treatment, (c, d) after the etching of mixed acid V(65wt% HNO3):V(H2O)=(e, f) 1: 0 (g, h) 1:1; (i, j) 1:5 and (k, l) 1:10

Fig. 4 XRD patterns of porous titanium subjected to HNO3 aqueous solution treatment after immersion in SBF V(65wt% HNO3):V(H2O) =(a) 1:0; (b) 1:1; (c) 1:5 and (d) 1:10

Fig. 5 Proliferation of MG63s grown on different porous titanium surface at 2, 4 and 6 d (P<0.05)

Fig. 6 FESEM micrographs showing the morphology of MG63s cultured on different porous titanium surface at 3 d

Fig. 7 CLSM micrographs showing the morphology of MG63s cultured on the different porous titanium surface (a, d) no treatment (b, e) V(65wt% HNO3):V(H2O)=1:1 (c, f) V(65wt% HNO3):V(H2O)=1:5 nitric treating

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不同浓度硝酸处理的多孔钛及其体外生物活性

Porous Titanium Treated by Nitric Acid with Varied Concentration and the Bioactivity in Vitro

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