成纤维细胞和血小板在多壁碳纳米管上粘附性

doi:10.3724/SP.J.1077.2011.00073

无机材料学报 ›› 2011, Vol. 26 ›› Issue (1): 73-77.DOI: 10.3724/SP.J.1077.2011.00073 CSTR: 32189.14.SP.J.1077.2011.00073

成纤维细胞和血小板在多壁碳纳米管上粘附性

赵梦鲤¹, 岳玉琛¹, 袁丽¹, 李德军¹, 吕晓迎², 黄炎²

1. 天津师范大学物理与电子信息学院, 天津300387; 2. 东南大学生物电子学国家重点实验室, 南京 210096

收稿日期:2010-04-28 修回日期:2010-06-05 出版日期:2011-01-20 网络出版日期:2010-12-23
作者简介:赵梦鲤(1986-), 女, 硕士研究生. E-mail: Zhaomengli_56@163.com
基金资助:
国家自然科学基金(11075116); 东南大学生物电子学国家重点实验室开放研究基金(2010-2012); 射线束技术与材料改性教育部重点实验室开放基金(201014)

Study of Fibroblast and Platelet Adhesion on Multi-wallCarbon Nanotubes

ZHAO Meng-Li¹, YUE Yu-Chen¹, YUAN Li¹,LI De-Jun¹, LÜ Xiao-Ying², HUANG Yan²

1. College of Physics and Electronic Information Science, Tianjin NormalUniversity, Tianjin 300387, China; 2. State Key Laboratory ofBioelectronics, Southeast University, Nanjing 210096, China

Received:2010-04-28 Revised:2010-06-05 Published:2011-01-20 Online:2010-12-23
Supported by:
National Natural Science Foundation ofChina(11075116); Open Research Fund of State Key Laboratory of Bioelectronics, Southeast University(2010-2012); Key Laboratory of Beam Technology and Material Modification of Ministry of Education, Beijing Normal University(201014)

摘要/Abstract

摘要： 使用化学气相沉积(CVD)的方法在碳纸基底上制备了多壁碳纳米管(MWCNTs), 并研究了L929小鼠成纤维细胞在多壁碳纳米管和碳纸对照组上的粘附及增殖等生长行为, 以及各种血液蛋白吸附于这两种材料表面后对人皮肤成纤维细胞粘附产生的影响, 同时对比了这两种材料的血小板粘附情况. 结果表明: 种植在多壁碳纳米管上的成纤维细胞生长明显比碳纸上的旺盛, 细胞浓度从第1天的12.5´10⁵/mL明显增加到第7天的4.1´10⁵/mL, 多壁碳纳米管对细胞无毒性反应. 预吸附白蛋白、纤维蛋白原、免疫球蛋白对细胞粘附量有促进作用, 并且多壁碳纳米管的血小板粘附率低于碳纸. 这些结果证明多壁碳纳米管具有良好的组织相容性和一定的血液相容性.

关键词: 多壁碳纳米管, 细胞粘附, 蛋白吸附, 血小板粘附

Abstract: Abstract:Multi-walled nanotubes (MWCNTs) were grown on carbonpapers by chemical vapor deposition (CVD). Adhesion and proliferation of L929 mouse fibroblasts and plateletson MWCNTs and carbon papers were investigated. The influence of different bloodprotein adsorption on the human skin fibroblasts growth was also studied. The resultsshowed that mouse fibroblasts implanted on MWCNTs tended to grow more prolificallythan those on carbon papers. The cell concentration observed on MWCNTsincreased from 1.25´10⁵/mL (cultured for 1 d) to 4.1´10⁵/mL (culture for 7 d). No toxicity reaction was observed during the culturingperiod.By employing the adsorptionassay, it was found albumin, fibrinogen, and immunoglobulinG adhered on materials could promotethe adhesion and growth of the human skin fibroblasts. Furthermore, plateletadhesion rate on MWCNTs is lower than that on the carbon papers. These resultsindicate that MWCNTs have better tissue compatibility and blood compatibility.

Key words: multi-wall carbon nanotubes, cell adhesion, proteinadsorption, platelet adhesion

中图分类号:

TB34
Q819

赵梦鲤, 岳玉琛, 袁丽, 李德军, 吕晓迎, 黄炎. 成纤维细胞和血小板在多壁碳纳米管上粘附性[J]. 无机材料学报, 2011, 26(1): 73-77.

ZHAO Meng-Li, YUE Yu-Chen, YUAN Li,LI De-Jun, LÜ Xiao-Ying, HUANG Yan. Study of Fibroblast and Platelet Adhesion on Multi-wallCarbon Nanotubes[J]. Journal of Inorganic Materials, 2011, 26(1): 73-77.

参考文献

[1] Sander C G L, John A J, Jos M, et al. Trends in biomaterials research: an analysis of the scientific program of the World Biomaterials Congress 2008. Biomaterials, 2008, 29(21): 3047-3052.

[2] Chor Y T, Gu H G, Leong W S, et al. Cellular behavior of human mesenchymal stem cells cultured on single-walled carbon nanotube film. Carbon, 2010, 48(4): 1095-1104.

[3] Eva H, Alan C, Fiona M L, et al. A new approach to the toxicity testing of carbon-based nanomaterials-the clonogenic assay. Toxicology Letters, 2007, 174(1/2/3): 49-60.

[4] Karin P, Jorg M W, Frank H, et al. Human lung epithelial cells show biphasic oxidative burst after single-walled carbon nanotube contact. Carbon, 2007, 45(11): 2241-2249.

[5] Xiao C W, Zhang W J, Ramaswami S, et al. Non-functionalized carbon nanotube binding with hemoglobin. Colloids and Surfaces B: Biointerfaces, 2008, 65(1): 146-149.

[6] Alla L A, Shan W Q, Pavel B, et al. Single-walled carbon nanotubes dispersed in aqueous media via non-covalent functionalization: effect of dispersant on the stability, cytotoxicity, and epigenetic toxicity of nanotube suspensions. Water Research, 2010, 44(2): 505-520.

[7] Laura E V, Pablo A F, Carlos D G, et al. The adsorption-desorption process of bovine serum albumin on carbon nanotubes. Journal of Colloid and Interface Science, 2007, 307(2): 349-356.

[8] Curtis A, Wikinsion C. Topographical control of cells. Biomaterials, 1997, 18(24): 1573-1583.

[9] Kenichi M, Frank X W, Masao Y, et al. The attachment and growth behavior of osteoblast-like cells on microtextured surfaces. Biomaterials, 2003, 24(16): 2711-2719.

[10] Puja C, Ajit S, Anju S. Cisplatin primes murine peritoneal macrophages for enhanced expression of nitric oxide, proinflammatory cytokines, TLRs, transcription factors and activation of MAP kinases upon co-incubation with L929 cells. Immunobiology, 2009, 214(3): 197-209.

[11] Van K T G, Spijker H T, Busscher H J. Plasma-treated polystyrene surfaces: model surfaces for studying cell-biomaterial interactions. Biomaterials, 2004, 25(10): 1735-1747.

[1]	刘甜甜, 王庆华, 刘希莉, 高凤, 汪庆祥. 近球状钼酸镍/多壁碳纳米管复合材料的制备及其赝电容性能[J]. 无机材料学报, 2018, 33(7): 735-740.
[2]	陈健, 郑玉婴, 张延兵, 邹海强, 卢秀恋. 氧化还原沉淀法制备MnO₂/MWCNTs催化剂及其低温SCR活性[J]. 无机材料学报, 2016, 31(12): 1347-1354.
[3]	付亚康, 周雪, 肖东琴, 匙峰, 卢晓英, 翁杰. 羟基磷灰石微纳结构对蛋白吸附的影响[J]. 无机材料学报, 2015, 30(5): 523-528.
[4]	陈峰, 孙团伟, 漆超, 吴进, 崔大祥, 朱英杰. 微波辅助溶剂热法制备磷酸钙微球和多面体[J]. 无机材料学报, 2014, 29(7): 776-780.
[5]	肖兴中, 易清风. MnO₂/SMWCNT/PANI三元复合材料的合成及其电化学电容性能[J]. 无机材料学报, 2013, 28(8): 825-830.
[6]	王婧, 陈颖, 朱向东, 樊渝江, 张兴栋. 双相磷酸钙陶瓷表面吸附血清蛋白的洗脱特性研究[J]. 无机材料学报, 2013, 28(10): 1143-1147.
[7]	张翔, 周大利, 龙沁, 周加贝, 谭言飞, 柳淑婧. 磷灰石-硅灰石生物活性玻璃陶瓷表面接枝多肽改性研究[J]. 无机材料学报, 2013, 28(10): 1137-1142.
[8]	吴振奕, 杨绳岩. 多壁碳纳米管-酞菁铜复合物的合成表征及光电性能[J]. 无机材料学报, 2011, 26(8): 785-791.
[9]	毕成, 朱美芳, 张青红, 李耀刚, 王宏志. 电磁波吸收剂BaTiO₃/MWCNT及其与PAN杂化纤维的制备与性能研究[J]. 无机材料学报, 2010, 25(8): 829-834.
[10]	张慧杰, 朱向东, 王辛龙, 范红松, 张兴栋. 烧结方式对羟基磷灰石陶瓷颗粒性能和蛋白吸附的影响[J]. 无机材料学报, 2010, 25(7): 770-774.
[11]	庞来学, 孙康宁, 李晋, 张金升. 多壁碳纳米管强韧化Fe₃Al复合材料[J]. 无机材料学报, 2010, 25(7): 733-737.
[12]	卿玉长,周万城,罗发,朱冬梅. 多壁碳纳米管/环氧有机硅树脂吸波涂层的介电和吸波性能研究[J]. 无机材料学报, 2010, 15(2): 181-185.
[13]	赵薇,黄可龙,刘素琴. 多壁碳纳米管对SiO电极电化学性能的影响[J]. 无机材料学报, 2008, 23(3): 578-582.

成纤维细胞和血小板在多壁碳纳米管上粘附性

Study of Fibroblast and Platelet Adhesion on Multi-wallCarbon Nanotubes

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 13

编辑推荐

Metrics

本文评价