双相磷酸钙陶瓷表面吸附血清蛋白的洗脱特性研究

doi:10.3724/SP.J.1077.2013.13047

无机材料学报 ›› 2013, Vol. 28 ›› Issue (10): 1143-1147.DOI: 10.3724/SP.J.1077.2013.13047 CSTR: 32189.14.SP.J.1077.2013.13047

双相磷酸钙陶瓷表面吸附血清蛋白的洗脱特性研究

王婧, 陈颖, 朱向东, 樊渝江, 张兴栋

(四川大学国家生物医学材料工程技术研究中心, 成都610064)

收稿日期:2013-01-17 修回日期:2013-03-26 出版日期:2013-10-20 网络出版日期:2013-09-18
作者简介:王婧(1988-), 女, 硕士研究生. E-mail: jw881010@163.com
基金资助:
国家自然科学基金 (81190131); 973计划 (2011CB606201)

Study on the Elution of the Adsorbed Serum Proteins on Biphasic Calcium Phosphate Ceramics

WANG Jing, CHEN Ying, ZHU Xiang-Dong, FAN Yu-Jiang, ZHANG Xing-Dong

(National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, China)

Received:2013-01-17 Revised:2013-03-26 Published:2013-10-20 Online:2013-09-18
About author:WANG Jing. E-mail: jw881010@163.com
Supported by:
National Natural Science Foundation of China (81190131); 973 Program (2011CB606201)

摘要/Abstract

摘要： 通过两种不同多孔结构的双相磷酸钙(biphasic calcium phosphate, BCP)陶瓷的制备, 以人血清为蛋白溶液模型, 选择三种不同的洗脱液对材料表面吸附的血清蛋白进行洗脱, 考察不同洗脱液及材料多孔结构对于BCP陶瓷表面吸附血清蛋白洗脱效果的影响。结果显示, 相对于2 mol/L氯化钠(NaCl)和 2%十二烷基磺酸钠溶液, 400 mmol/L 磷酸钠(Na₃PO₄)溶液对于BCP陶瓷表面吸附的血清蛋白洗脱效果更佳。此外, 材料多孔结构在血清蛋白的吸附和洗脱中起重要作用, 微孔结构能促进BCP陶瓷表面的血清蛋白吸附并减缓吸附蛋白的释放速率, 而三维贯通的大孔结构则有利于材料表面吸附血清蛋白的洗脱和释放。

关键词: BCP陶瓷, 多孔结构, 洗脱液, 蛋白吸附, 洗脱

Abstract: Two types of porous BCP ceramics were fabricated, using human serum as the model of protein solution. The effect of the different eluants and porous structure on the elution of the adsorbed serum proteins on BCP ceramics were investigated. The results showed that 400 mmol/L Na₃PO₄ solution had higher elution efficiency for the adsorbed serum proteins on BCP ceramics than 2 mol/L NaCl or 2% sodium dodecycl sufonate (SDS) solution. Moreover, the porous structure of BCP ceramics had a strong impact on the elution of the adsorbed serum proteins. The microporous structure could promote the adsorption of serum proteins on BCP ceramics and slowed down their release rate, but the 3D interconnected macropores were favorable for the elution and release of serum proteins from the BCP surface.

Key words: BCP ceramics, porous structure, eluants, protein adsorption, elution

中图分类号:

R318

王婧, 陈颖, 朱向东, 樊渝江, 张兴栋. 双相磷酸钙陶瓷表面吸附血清蛋白的洗脱特性研究[J]. 无机材料学报, 2013, 28(10): 1143-1147.

WANG Jing, CHEN Ying, ZHU Xiang-Dong, FAN Yu-Jiang, ZHANG Xing-Dong. Study on the Elution of the Adsorbed Serum Proteins on Biphasic Calcium Phosphate Ceramics[J]. Journal of Inorganic Materials, 2013, 28(10): 1143-1147.

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双相磷酸钙陶瓷表面吸附血清蛋白的洗脱特性研究

Study on the Elution of the Adsorbed Serum Proteins on Biphasic Calcium Phosphate Ceramics

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