磷灰石-硅灰石生物活性玻璃陶瓷表面接枝多肽改性研究

doi:10.3724/SP.J.1077.2013.13090

无机材料学报 ›› 2013, Vol. 28 ›› Issue (10): 1137-1142.DOI: 10.3724/SP.J.1077.2013.13090 CSTR: 32189.14.SP.J.1077.2013.13090

磷灰石-硅灰石生物活性玻璃陶瓷表面接枝多肽改性研究

张翔, 周大利, 龙沁, 周加贝, 谭言飞, 柳淑婧

(四川大学材料科学与工程学院, 成都 610064)

收稿日期:2013-02-19 修回日期:2013-04-02 出版日期:2013-10-20 网络出版日期:2013-09-18
作者简介:张翔(1986-), 男, 博士研究生. E-mail: zhang_npu@yahoo.cn
基金资助:
高等学校博士学科点专项科研基金(20060610024)

Surface Modification of Apatite-wollastonite Bioactive Glass-ceramic by Grafting with RGD Peptides

ZHANG Xiang, ZHOU Da-Li, LONG Qin, ZHOU Jia-Bei, TAN Yan-Fei, LIU Shu-Jing

(College of Materials Science and Engineering, Sichuan University, Chengdu 610064, China)

Received:2013-02-19 Revised:2013-04-02 Published:2013-10-20 Online:2013-09-18
About author:ZHANG Xiang. E-mail: zhang_npu@yahoo.cn
Supported by:
Specialized Research Fund for the Doctoral Program of Higher Education of China (20060610024)

摘要/Abstract

摘要： 为在磷灰石-硅灰石生物活性玻璃陶瓷(Apatite-Wollastonite Bioactive Glass-Ceramic, AW)表面引入能够促进细胞粘附的RGD(精氨酸-甘氨酸-天冬氨酸)多肽以提高其生物活性, 采用低温等离子法在材料的表面引入活性氨基基团, 并通过浸渍法使氨基基团与多肽发生反应。采用XRD、XPS、ATR-FTIR对AW的相组成及表面改性特性进行表征, 确认通过低温等离子法在AW表面接上氨基, RGD多肽分子与氨基反应以化学键合的形式接枝到材料表面(RGD-AW), 实现了在AW表面接枝生物大分子的改性。将改性前后的材料分别与类成骨细胞(MG63细胞)混合培养并使用荧光显微镜、SEM及MTT等测试方法对材料的细胞生物学性能进行了表征。细胞实验结果表明: 接枝RGD多肽分子的材料在细胞培养的早期阶段比AW更有利于细胞的粘附及铺展。

关键词: AW生物活性玻璃陶瓷, 氨基, 表面改性, 等离子接枝, RGD固定, 细胞粘附

Abstract: Tripeptide of arginine-glycine-aspartic acid (RGD) has been introduced to modify of biomaterials due to its high bio-activity, which could increase the cell adhesion. In this study, the apatite-wollastonite bioactive glass-ceramic (AW) with RGD peptide (RGD-AW) was fabricated. Firstly, the amino groups were introduced to the AW surface via low temperature plasma treatment. Then, RGD peptide were immobilized on the surface of AW by combination with the amino groups. The XRD, XPS and ATR-FTIR results confirmed that the RGD peptide were fixed on the surface of AW successfully. In order to testify whether the RGD-AW were more favorable to the cells, MG63 osteblast-like cells were employed to co-culture with RGD-AW for 4 h, 1 d and 5 d in vitro. The results of fluorescence microscopy, SEM and MTT assay showed that RGD-AW could enhance the attachment and proliferation of MG63 cells significantly compared with AW.

Key words: apatite-wollastonite bioactive glass-ceramic, amino-group, surface modification, plasma induced grafting, RGD immobilization, cell adhesion

中图分类号:

TQ174

张翔, 周大利, 龙沁, 周加贝, 谭言飞, 柳淑婧. 磷灰石-硅灰石生物活性玻璃陶瓷表面接枝多肽改性研究[J]. 无机材料学报, 2013, 28(10): 1137-1142.

ZHANG Xiang, ZHOU Da-Li, LONG Qin, ZHOU Jia-Bei, TAN Yan-Fei, LIU Shu-Jing. Surface Modification of Apatite-wollastonite Bioactive Glass-ceramic by Grafting with RGD Peptides[J]. Journal of Inorganic Materials, 2013, 28(10): 1137-1142.

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磷灰石-硅灰石生物活性玻璃陶瓷表面接枝多肽改性研究

Surface Modification of Apatite-wollastonite Bioactive Glass-ceramic by Grafting with RGD Peptides

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