无机材料学报 ›› 2015, Vol. 30 ›› Issue (5): 523-528.DOI: 10.15541/jim20140510 CSTR: 32189.14.10.15541/jim20140510

• 研究论文 • 上一篇    下一篇

羟基磷灰石微纳结构对蛋白吸附的影响

付亚康1, 2, 周 雪1, 肖东琴1, 匙 峰1, 卢晓英1, 翁 杰1   

  1. (1. 西南交通大学 材料先进技术教育部重点实验室, 成都 610031; 2. 雅安职业技术学院, 雅安 625000)
  • 收稿日期:2014-10-10 修回日期:2014-11-20 出版日期:2015-05-20 网络出版日期:2015-04-28
  • 作者简介:付亚康(1988–), 男, 硕士研究生, 助教. E-mail: fykmail@163.com; 810667526@qq.com
  • 基金资助:

    国家重点基础研究发展计划(2012CB933600); 国家自然科学基金(51172188); 四川省科技支撑计划(2010FZ0048); 四川省高校科研创新团队建设计划项目(14TD0050)

Influence of Micro-nano Structure of Haydroxyapatite Particles on Protein Adsorption

FU Ya-Kang1, 2, ZHOU Xue1, XIAO Dong-Qin1, SHI Feng1, LU Xiao-Ying1, WENG Jie1   

  1. (1. Key Laboratory of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China; 2. Ya’an Vocational College, Ya’an 625000, China)
  • Received:2014-10-10 Revised:2014-11-20 Published:2015-05-20 Online:2015-04-28
  • About author:FU Ya-Kang. E-mail: fykmail@163.com; 810667526@qq.com
  • Supported by:

    National Basic Research Program of China (2012CB933600); National Natural Science Foundation of China (51172188); Science and Technology Pillar Project of Sichuan (2010FZ0048); Research and Innovation Team Project of Sichuan for Central Universities(14TD0050)

摘要:

本研究采用水热反应法, 在不同浓度环己烷六羧酸(H6E)模板调控作用下, 合成了具有不同表面微纳结构的羟基磷灰石(HAP)微粒, 并采用XRD、BET、FTIR和SEM对其进行表征。对HAP微粒进行了牛血清白蛋白(BSA)、纤维蛋白原(FN)和溶菌酶(LYS)的吸附及释放实验。结果表明: H6E能够在HAP微粒表面构建微纳结构, 不同微纳结构对不同蛋白质具有选择性吸附作用; 在H6E浓度为50 mmol/L的合成条件下制备的中空结构HAP微粒(HAP50)其载蛋白后体外释放具有明显的蛋白缓释性能。

关键词: 羟基磷灰石, 环己烷六羧酸, 微纳结构, 蛋白吸附

Abstract:

Hydroxyapatite (HAP) particles were hydrothermally synthesized with the surface morphologies adjusted by cyclohexane-1, 2, 3, 4, 5, 6-hexacarboxylic acid (H6E) as the template. HAP particles were characterized by XRD, BET, SEM and FTIR. The protein adsorption-desorption behaviors of positively charged lysozyme (LYS), fibrinogen (FN) and negatively charged bovine serum albumin (BSA) on these HAP particles were examined. The results indicate that using H6E as a template to fabricate micro-nano structures on HAP particles through hydrothermal reaction is simple and controllable. HAP particles with micro-nano structures show selective protein adsorption-desorption properties for different proteins. The protein-loaded shell-like HAP particle (HAP50-protein) shows an excellent protein release behavior in vitro.

Key words: hydroxyapatite, H6E, micro-nano structure, protein adsorption

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