Controllable Preparation and in Vitro Bioactivity of Bioglass Microspheres via Spray Drying Method

doi:10.15541/jim20190621

Abstract

Abstract: The CaO-SiO₂-P₂O₅ bioglass (BG) microspheres with good bioactivity and osteoconductivity have been extensively studied for bone tissue engineering. Traditional melting method for preparing BG powders is high energy consumption, and difficult to control the morphology of BG. While Sol-Gel technique for BG preparation requires a large amount of organic solvent, long preparation period and low mass production. For the rapid and scalable preparation of BG microspheres with controllable morphology, particle size and chemical composition, an efficient strategy of spray drying of precursor solution was proposed in this study, and the effects of process parameters including inlet air volume, precursor solution concentration and feed rate on the particle size of BG microspheres, and the effect of chemical composition on the apatite formation ability of BG microspheres in vitro were investigated. The results showed that the particle sizes of BG microspheres via spray drying could be controlled below 40 μm, and the particle sizes of BG microspheres increased with the concentration of precursor solution, and decreased with the increase of inlet air volume, but the feed rate has a slight effect on the particle size of the microspheres. On the other hand, BG microspheres with different chemical compositions had good ability to induce apatite formation in vitro, and it increased with the increase of CaO content. Therefore, spray drying method would be promising for rapid and scalable preparation of BG microspheres.

Key words: spray drying, bioglass, microsphere, apatite

CLC Number:

TQ174

HU Yaping, TIAN Zhengfang, ZHU Min, ZHU Yufang. Controllable Preparation and in Vitro Bioactivity of Bioglass Microspheres via Spray Drying Method[J]. Journal of Inorganic Materials, 2020, 35(11): 1268-1276.

References

[1] JONES J R.Review of bioactive glass: From Hench to hybrids.Acta Biomaterialia, 2015, 23: S53-S82.
[2] HULBERT S F, HENCH L L, FORBERS D,et al. The story of bioceramics. Ceramics International, 1982, 8(4): 131-140.
[3] FU S, HU H, CHEN J,et al. Silicone resin derived larnite/C scaffolds via 3D printing for potential tumor therapy and bone regeneration. Chemical Engineering Journal, 2020, 382: 122928.
[4] FU S Y, ZHU M, ZHU Y F.Organosilicon polymer-derived ceramics: an overview.Journal of Advanced Ceramics, 2019, 8(4): 457-478.
[5] ZHANG J J, CHEN X, LIN C, et al. Preparation of nanoscale bioactive glasses by addition of PEG as surface dispersion agent. Bulletin of the Chinese Ceramic Society, 2010, 29(2): 257-261.
[6] CHEN X, MENG Y, LI Y.Investigation on bio-mineralization of melt and Sol-Gel derived bioactive glasses.Applied Surface Science, 2008, 255(2): 562-564.
[7] ZHONG J P, GREENSPAN D C. Processing and properties of Sol- Gel bioactive glasses. Journal of Biomedical Materical Research, 2000, 53(6): 694-701.
[8] PAN S, YIN J, YU L,et al. 2D MXene-integrated 3D-printing scaffolds for augmented osteosarcoma phototherapy and accelerated tissue reconstruction. Advanced Science, 2020, 7: 1901551.
[9] DU X, WEI D, HUANG L,et al. 3D printing of mesoporous bioactive glass/silk ﬁbroin composite scafolds for bone tissue engineering. Materials Science & Engineering C, 2019, 103: 109731.
[10] PONTIROLI L, DADKHAH M, NOVAJRA G, et al. An aerosol- spray-assisted approach to produce mesoporous bioactive glass microspheres under mild acidic aqueous conditions. Materials Letters, 2017, 190: 111-114.
[11] YAMAUCHI Y, SUZUKI N, GUPTA P,et al. Aerosol-assisted synthesis of mesoporous organosilica microspheres with controlled organic contents. Science and Technology of Advanced Materials, 2009, 10(2): 025005-025009.
[12] FIORILLI S, TALLIA F, Pontiroli L,et al. Spray-dried mesoprous silica spheres functionalized with carboxylic groups. Materials Letters, 2013, 108: 118-121.
[13] LIU W J, WU W D, SELOMULYA C,et al. A single step assembly of uniform microparticles for controlled release applications. Soft Matter, 2011, 7(7): 3323.
[14] WU W D, AMELIA R, HAO N,et al. Assembly of uniform photoluminescent microcomposites using a novel micro-fluidic-jet- spray-dryer. AIChE Journal, 2011, 57: 2726-2737.
[15] AMELIA R, WU W D, CASHION J,et al. Microfluidic spray drying as a versatile assembly route of functional particles. Chemical Engineering Science, 2011, 66(22): 5531-5540
[16] AMELIA R, WU W D, CHEN X D,et al. Assembly of magnetic microcomposites from low pH precursors using a novel micro-fluidic-jet-spray-dryer. Chemical Engineering Research & Design, 2012, 90(1): 150-157.
[17] LIU W J, WU W D, SELOMULYA C,et al. Facile spray-drying assembly of uniform microencapsulates with tunable core-shell structures and controlled release properties. Langmuir, 2011, 27(21): 12910-12915.
[18] SHIH S J, Chou Y J, BORISENKO K B.Preparation method: structure-bioactivity correlation in mesoporous bioactive glass.Journal of Nanoparticle Research, 2013, 15(6): 1763.
[19] OSTOMEL T, SHI Q, TSUNG C K,et al. Spherical bioactive glass with enhanced rates of hydroxyapatite deposition and hemostatic activity. Small, 2006, 2(11): 1261-1265.
[20] ARCOS D, LOPEZ-NORIEGA A, RUIZ-HERNA NDEZ E,et al. Ordered mesoporous microspheres for bone grafting and drug delivery. Chemistry of Materials, 2009, 21(6): 1000-1009.
[21] WU W D, LIU W J, SELOMULYA C,et al. On spray drying of uniform silica-based microencapsulates for controlled release. Soft Matter, 2011, 7(24): 11416-11424.
[22] KOKUBO T, TAKADAMA H.How useful is SBF in predictingin vivo bone bioactivity? Biomaterials, 2006, 27(15): 2907-2915.
[23] MASTERS K.Spray Drying Handbook, fifth ed. Harlow: Longman Scientific and Technical, 1991, 15(3): 344-345.
[24] LAVERNIA E J, WU Y.Spray atomization and deposition. Journal of Materials Processing Technology, 2000: 278-279
[25] NUKIYAMA S, TANASAWA Y.Experiments on the atomization of liquids in an airstream.Transactions of the Canadian Society for Mechanical Engineering, 1940: S7.
[26] IDE M, WALLAERT E, DRIESSCHE I V,et al. Spherical mesopor- ous silica particles by spray drying: doubling the retention factor of HPLC columns. Microporous & Mesoporous Materials, 2011, 142(1): 282-291.
[27] FUJITA Y, YAMAMURO T, NAKAMURA T,et al. The bonding behavior of calcite to bone. Journal of Biomedical Materical Research, 1991, 25: 991-1003.
[28] ŚLÓSARCZYK A, PASZKIEWICZ Z, PALUSZKIEWICZ C. FTIR and XRD evaluation of carbonated hydroxyapatite powders synthesized by wet methods.Journal of Molecular Structure, 2005, 744: 657-661.
[29] MEYER J L, FOWLER B O.Lattice defects in nonstoichiometric calcium hydroxylapatites: a chemical approach. Inorganic Chemistry, 1982, 21(8): 3029-3035.
[30] MIAO X, TAN L P, TAN L S,et al. Porous calcium phosphate ceramics modified with PLGA-bioactive glass. Materials Science and Engineering, 2007, 27(2): 274-279.
[31] RICHARD M D, ALDO R B.Effect of particulate bioactive glasses on human macrophages and monocytesin vitro. Journal of Biomedical Materical Research Part A, 2005, 73A(1): 73-79.
[32] LUTZ-CHRISTIAN G, BOCCACCINI A R.Review - bioactive glass and glass-ceramic scaffolds for bone tissue engineering.Materials, 2010, 3(7): 3867-3910.
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