hBMP-2 Contained Composite Coatings on Titanium Mesh Surface: Preparation and hBMP-2 Release

doi:10.15541/jim20190127

Abstract

Abstract:

Titanate nanofibers were prepared on titanium mesh by alkali-heat treatment, and calcium phosphate coating was fabricated on the porous titanate nanofibers by electrochemical deposition technology. Then hBMP-2 was introduced into the coating by different methods to improve its osteointegration and bioactivity. Three kinds of composite coatings modified by hBMP-2 were prepared (TmhB, TmHedhB and TmHhBed). Surface morphology, chemical composition, phase composition and hBMP-2 amounts and hBMP-2 release performance of the composite coatings were characterized by SEM, ATR-FTIR, XRD, and hBMP-2 ELISA kit, respectively. Results showed that all of the coatings display porous fiber structure, calcium phosphate phase in TmHedhB and TmHhBed samples was hydroxyapatite (HA), and bead-like HA particles formed on the surface of titanate nanofibers. Protein adsorption experiments showed that introduction of bead-like HA phase increased the hBMP-2 adsorption on the composite coatings, and composite coatings prepared by electrochemical co-deposition technique further enhanced hBMP-2 adsorption up to 886 ng/mg, which were supported hBMP-2 sustained release within 6-48 h.

Key words: titanium, alkali-heat treatment, electrochemical deposition, hydroxyapatite, composite coating, hBMP-2

CLC Number:

R318
TQ174

FU Ya-Kang,WENG Jie,LIU Yao-Wen,ZHANG Ke-Hong. hBMP-2 Contained Composite Coatings on Titanium Mesh Surface: Preparation and hBMP-2 Release[J]. Journal of Inorganic Materials, 2020, 35(2): 173-178.

Figures/Tables 7

Fig. 1 Schematic diagram of preparation of composite coating on titanium mesh surface by electrochemical co-deposition

Table 1 Special fabrication procedures of titanium mesh after alkali-heat treatment

Sample	Special fabrication procedure
TmhB	hBMP-2 adsorption
TmHedhB	HA electrochemical deposition followed by hBMP-2 adsorption
TmHhBed	hBMP-2/HA electrochemical co-deposition

Fig. 2 ATR-FTIR spectra of different composite coatings on titanium mesh surface

Fig. 3 XRD images of different composite coatings on titanium mesh surface

Fig. 4 SEM images of different composite coatings on titanium mesh surface (a-c) TmhB; (d-f) TmHedhB; (g-i) TmHhBed

Fig. 5 hBMP-2 content in different composite coatings on titanium mesh surface (*P < 0.05)

Fig. 6 Release curves of hBMP-2 in different composite coatings on titanium mesh surface

References 43

[1]	BUSER D, SCHENK R K, STEINEMANN S , et al. Influence of surface characteristics on bone integration of titanium implants. a histomorphometric study in miniature pigs. Journal of Biomedical Materials Research, 1991,25(7):889-902.
[2]	DABROWSKI B, SWIESZKOWSKI W, GODLINSKI D , et al. Highly porous titanium scaffolds for orthopaedic applications. Journal of Biomedical Materials Research Part B Applied Biomaterials, 2010,95B(1):53-61.
[3]	CARINE V, YANN C, DIDIER L O , et al. Surface modification of titanium with phosphonic acid to improve bone bonding: characterization by XPS and ToF-SIMS. Langmuir, 2002,18(7):2582-2589.
[4]	WANG X H, LI J S, HU R , et al. Mechanical properties and bioactive surface modification via alkali-heat treatment of porous titanium for biomedical applications. Advanced Materials Research, 2013,647:511-517.
[5]	DAS K, BALLA V K, BANDYOPADHYAY A , et al. Surface modification of laser-processed porous titanium for load-bearing implants. Scripta Materialia, 2008,59(8):822-825.
[6]	RAKHMATIA Y D, AYUKAWA Y, FURUHASHI A , et al. Current barrier membranes: titanium mesh and other membranes for guided bone regeneration in dental applications. J. Prosthodont. Res., 2013,57(1):3-14.
[7]	MARBACHER S, ANDRES R H, FATHI A R , et al. Primary reconstruction of open depressed skull fractures with titanium mesh. Journal of Craniofacial Surgery, 2008,19(2):490-495.
[8]	SCH N R, METZGER M C, ZIZELMANN C , et al. Individually preformed titanium mesh implants for a true-to-original repair of orbital fractures. International Journal of Oral & Maxillofacial Surgery, 2006,35(11):990-995.
[9]	NAKASE H, PARK Y S, KIMURA H , et al. Complications and long-term follow-up results in titanium mesh cage reconstruction after cervical corpectomy. Journal of Spinal Disorders & Techniques, 2006,19(19):353-357.
[10]	MARTIN M P, OLSON S . Post-operative complications with titanium mesh. Journal of Clinical Neuroscience, 2009,16(8):1080-1081.
[11]	CARRAD A . Development of bioactive hydroxyapatite coatings on titanium alloys. Key Engineering Materials, 2012,533:183-193.
[12]	LANGELIER B, WANG X, GRANDFIELD K . Atomic scale chemical tomography of human bone. Scientific Reports, 2017,7:39958.
[13]	KANE R, MA P X . Mimicking the nanostructure of bone matrix to regenerate bone. Materials Today, 2013,16(11):418-423.
[14]	HABIBOVIC P, YUAN H, VAN D D M , et al. Relevance of osteoinductive biomaterials in critical-sized orthotopic defect. Journal of Orthopaedic Research, 2010,24(5):867-876.
[15]	LI C, VEPARI C, JIN H J , et al. Electrospun silk-BMP-2 scaffolds for bone tissue engineering. Biomaterials, 2006,27(16):3115-3124.
[16]	MURPHY C M, SCHINDELER A, GLEESON J P , et al. A collagen- hydroxyapatite scaffold allows for binding and co-delivery of recombinant bone morphogenetic proteins and bisphosphonates. Acta Biomaterialia, 2014,10(5):2250-2258.
[17]	GUO X, PARK H, YOUNG S , et al. Repair of osteochondral defects with biodegradable hydrogel composites encapsulating marrow mesenchymal stem cells in a rabbit model. Acta Biomaterialia, 2010,6(1):39-47.
[18]	CHEN P C, XU Z K . Mineral-coated polymer membranes with superhydrophilicity and underwater superoleophobicity for effective oil/water separation. Sci. Rep., 2013,3(6153):2776.
[19]	USINSKAS P, STANKEVICIUTE Z, BEGANSKIENE A , et al. Sol-Gel derived porous and hydrophilic calcium hydroxyapatite coating on modified titanium substrate. Surface & Coatings Technology, 2016,307:935-940.
[20]	CHA C, LIECHTY W B, KHADEMHOSSEINI A , et al. Designing biomaterials to direct stem cell fate. ACS Nano, 2012,6(11):9353-9358.
[21]	LUTOLF M P, GILBERT P M, BLAU H M . Designing materials to direct stem-cell fate. Nature, 2009,462(7272):433-441.
[22]	DOM NGUEZ-TRUJILLO C, PE N E, CHICARDI E , et al. Sol-Gel deposition of hydroxyapatite coatings on porous titanium for biomedical applications. Surface & Coatings Technology, 2017,333(15):158-162.
[23]	PENG H, USAS A, OLSHANSKI A , et al. VEGF improves, whereas sFlt1 inhibits, BMP2-induced bone formation and bone healing through modulation of angiogenesis. Journal of Bone & Mineral Research, 2010,20(11):2017-2027.
[24]	CHEN L, JIANG W, HUANG J , et al. Insulin-like growth factor 2(IGF-2) potentiates BMP-9-induced osteogenic differentiation and bone formation. Journal of Bone & Mineral Research, 2010,47(11):S432-S433.
[25]	BOSE S, TARAFDER S . Calcium phosphate ceramic systems in growth factor and drug delivery for bone tissue engineering: a review. Acta Biomaterialia, 2012,8(4):1401-1421.
[26]	KHAN S N, BOSTROM M P, LANE J M . Bone growth factors. Orthopedic Clinics of North America, 2000,31(3):375-387.
[27]	WANG Z, CHEN L, XU J , et al. Bioadhesive microporous architectures by self-assembling polydopamine microcapsules for biomedical applications. Chemistry of Materials, 2015,27(3):848-856.
[28]	ZHANG C D, XIAO D Q, FU Y K , et al. Fabrication of nanostructured hierarchical coatings composed of calcium phosphate/ titanate on titanium substrate. Key Engineering Materials, 2014, 575-576:253-258.
[29]	ZHAO H, DONG W, ZHENG Y , et al. The structural and biological properties of hydroxyapatite-modified titanate nanowire scaffolds. Biomaterials, 2011,32(25):5837-5846.
[30]	KOUTSOPOULOS S . Synthesis and characterization of hydroxyapatite crystals: a review study on the analytical methods. Journal of Biomedical Materials Research Part A, 2002,62(4):600-612.
[31]	PALAZZO B, IAFISCO M, LAFORGIA M , et al. Biomimetic hydroxyapatite-drug nanocrystals as potential bone substitutes with antitumor drug delivery properties. Advanced Functional Materials, 2010,17(13):2180-2188.
[32]	CAI L, LIN D, CHAI Y , et al. MBG scaffold containing chitosan microspheres as binary delivery system of IL-8 and BMP-2 for bone regeneration. Journal of Materials Chemistry B, 2018,6(27):4453-4465.
[33]	LU X, WANG Y B, LIU Y R , et al. Preparation of HA/chitosan composite coatings on alkali treated titanium surfaces through Sol-Gel techniques. Materials Letters, 2007,61(18):3970-3973.
[34]	PANG D, HE L, WEI L , et al. Preparation of a beta-tricalcium phosphate nanocoating and its protein adsorption behaviour by quartz crystal microbalance with dissipation technique. Colloids and Surfaces B: Biointerfaces, 2018,162:1-7.
[35]	DONG X, WANG Q, WU T , et al. Understanding adsorption- desorption dynamics of BMP-2 on hydroxyapatite(001) surface. Biophysical journal, 2007,93(3):750-759.
[36]	WALLWORK M L, KIRKHAM J, ZHANG J , et al. Binding of matrix proteins to developing enamel crystals: an atomic force microscopy study. Langmuir, 2001,17(8):2508-2513.
[37]	ZHU X, FAN H, LI D , et al. Protein adsorption and zeta potentials of a biphasic calcium phosphate ceramic under various conditions. J. Biomed. Mater. Res. B App.l Biomater., 2007,82(1):65-73.
[38]	ZHOU H, WU T, DONG X , et al. Adsorption mechanism of BMP-7 on hydroxyapatite(001) surfaces. Biochem. Biophys. Res. Commun., 2007,361(1):91-96.
[39]	KANDORI K, FUDO A, ISHIKAWA T . Study on the particle texture dependence of protein adsorption by using synthetic micrometer- sized calcium hydroxyapatite particles. Colloids and Surfaces B: Biointerfaces, 2002,24(2):145-153.
[40]	BOIX T, GOMEZ-MORALES J, TORRENT-BURGUES J , et al. Adsorption of recombinant human bone morphogenetic protein rhBMP-2m onto hydroxyapatite. Journal of Inorganic Biochemistry, 2005,99(5):1043-1050.
[41]	FU Y K, ZHOU X, XIAO D Q , et al. Influence of micro-nano structure of haydroxyapatite particles on protein adsorption. Journal of Inorganic Materials, 2015,30(5):523-528.
[42]	FU Y K, LI X Z, XIA X , et al. Effect of zeta potential of hydroxyapatite on protein adsorption. Progress in Modern Biomedicine, 2016,16(19):3610-3613.
[43]	IMAMURA T, KAITO T . Homeostasis and disorder of musculoskeletal system.BMP and TGF signaling and locomotive tissues. Clinical Calcium, 2018,28(3):313.