Litchi-like Superparamagnetic Hydroxyapatite Microspheres with Hierarchically Mesoporous Microspheres

doi:10.15541/jim20180497

Abstract

Abstract:

Due to the fact that the conventional bioceramic microspheres lack target function, novel litchi-like porous microspheres composed of a core of CaCO₃ and a tunable magnetic-hydroxyapatite (HA) shell were successfully prepared in this study. Antitumor drug, doxorubicin (DOX), was effectively loaded on the HA microspheres which possess magnetic targeting function. In addition, the HA shell, which had favorable biocompatibility and pH response characteristics, could be used to control release of loaded DOX from the litchi-like superparamagnetic microspheres in a simulated acidic tumor cell environment, effectively killing tumor cells and reducing toxic side effects to normal cells. The smart design presented in this study, which incorporates a tunable superparamagnetic shell and a controlled architecture, allows the sensitive release of drugs for efficient antitumor activity.

Key words: core-shell, microspheres, hydroxyapatite, DOX

CLC Number:

TQ174

XIAO Wen-Qian,ZHANG Jing,LI Ke-Jiang,ZOU Xin-Yu,CAI Yu-Dong,LI Bo,LIU Xue,LIAO Xiao-Ling. Litchi-like Superparamagnetic Hydroxyapatite Microspheres with Hierarchically Mesoporous Microspheres[J]. Journal of Inorganic Materials, 2019, 34(9): 925-932.

Figures/Tables 10

Fig. 1 SEM images of superparamagnetic CaCO3@HA/Fe3O4 microspheres with different Fe3O4 component. Note: S0 (a1, a2), S1 (b1, b2), S2 (c1, c2), S3 (d1, d2)

Fig. 2 TEM images before (a) and after (b) hydrothermal conversion of CaCO3@HA/Fe3O4 microspheres with 25wt% Fe3O4 component

Fig. 3 Typical EDS patterns of HA microspheres with 25wt% Fe3O4 component

Fig. 4 XRD patterns of CaCO3@HA/Fe3O4 microspheres with different Fe3O4 component

Fig. 5 Typical nitrogen isothermal adsorption curves (a) and mesopore distribution (b) analysis of CaCO3@HA/Fe3O4 microspheres with different Fe3O4 content

Fig. 6 Formation mechanism diagram of litchi-like superparamagnetic CaCO3@HA/Fe3O4 microspheres

Fig. 7 Digital photographs (a) of the litchi-like CaCO3@HA/Fe3O4 magnetic HA microspheres in aqueous suspension and (b) magnetization of different samples as a function of the applied field measured at 300 K

Table 1 Fe3O4 content, specific surface area (SBET), drug loading amount (DLA) and drug loading efficiency (DLE) of CaCO3@HA/Fe3O4 microspheres

Sample	Fe₃O₄ content/wt%	S_BET/(m²·g^-1)	DLA /(mg·g^-1)	DLE/%
S1	16.08	196.481	96.88	96.88
S2	17.69	50.749	96.14	96.14
S3	37.98	46.623	97.96	97.96

Fig. 8 DOX release profiles of the DOX-loaded litchi-like magnetic HA microspheres in PBS with different pH of 3.4, 5.7 and 7.3 at 37 ℃

Fig. 9 CCK-8 assay of HaCaT (a) and HN6 tumor cells (b) co-cultured with unloaded or DOX-loaded litchi-like magnetic HA microspheres and litchi-like HA microspheres for 24 h

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