Study on Hollow Hydroxyapatite Microspheres as Delivery System for Human Bone Morphogenetic Protein

doi:10.3724/SP.J.1077.2011.00974

Journal of Inorganic Materials ›› 2011, Vol. 26 ›› Issue (9): 974-978.DOI: 10.3724/SP.J.1077.2011.00974

• Research Paper • Previous Articles Next Articles

Study on Hollow Hydroxyapatite Microspheres as Delivery System for Human Bone Morphogenetic Protein

YAO Ai-Hua¹, XU Wei¹, AI Fan-Rong^1,3, CHEN Qi¹, WANG De-Ping^1,2, HUANG Wen-Hai¹, Xu Jun⁴

(1. School of Materials Science and Engineering, Tongji University, Shanghai 200092, China; 2. Key Laboratory of Advanced Civil Engineering Materials, Ministry of Education, Tongji University, Shanghai 200092, China; 3. College of Mechanical and Electrical Engineering, Nanchang University, Nanchang 330031, China; 4. Shanghai Sixth People’s Hospital affiliated Shanghai JiaoTong University, Shanghai 200233, China)

Received:2010-10-30 Revised:2010-12-15 Published:2011-09-20 Online:2011-08-19
Supported by:
Key Project on Basic Research of Shanghai (08JC1419200); Program for Young Excellent Talent in Tongji University; Scientific Research Foundation of State Education Ministry for the Return Overseas Chinese Scholars; National Natural Science Foundation of China (81000788)

Abstract

Abstract: Hollow hydroxyapatite microspheres consisted of a hollow core and a porous shell were prepared by converting Li₂O-CaO-B₂O₃glass microspheres in dilute phosphate solution at 37℃. Human bone morphogenetic protein (rhBMP-2) was loaded in the resultant microspheres, and the in vitro release behavior of rhBMP-2 was studied. Furthermore, cell culture technique and ALP kit were used to investigate the effect of the released rhBMP-2 on cell activity. After incubation with rhBMP-2 loaded microspheres, the alkaline phosphatase (ALP) activity of the rat bone marrow mesenchymal stem cells (MSCs) was evaluated. It was found that rhBMP-2 could be released slowly from the hollow HA microspheres for an extended period time up to 1000h. The present delivery system established possessed higher biological activity as compared with pure rhBMP-2.

Key words: hydroxyapatite, hollow microsphere, bone morphogenetic protein, drug delivery

CLC Number:

TB321

YAO Ai-Hua, XU Wei, AI Fan-Rong, CHEN Qi, WANG De-Ping, HUANG Wen-Hai, Xu Jun. Study on Hollow Hydroxyapatite Microspheres as Delivery System for Human Bone Morphogenetic Protein[J]. Journal of Inorganic Materials, 2011, 26(9): 974-978.

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Study on Hollow Hydroxyapatite Microspheres as Delivery System for Human Bone Morphogenetic Protein

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