致密羟基磷灰石球粒的制备及其生物学性能研究

doi:10.3724/SP.J.1077.2013.12038

无机材料学报 ›› 2013, Vol. 28 ›› Issue (1): 40-44.DOI: 10.3724/SP.J.1077.2013.12038 CSTR: 32189.14.SP.J.1077.2013.12038

致密羟基磷灰石球粒的制备及其生物学性能研究

罗会涛, 智伟, 鲁雄, 李金雨, 冯波, 翁杰

(西南交通大学材料科学与工程学院, 材料先进技术教育部重点实验室, 四川610031)

收稿日期:2012-01-13 修回日期:2012-03-16 出版日期:2013-01-10 网络出版日期:2012-12-20
作者简介:罗会涛(1985–), 男, 博士研究生. E-mail: luo815923@163.com
基金资助:
国家重大科学研究计划(2012CB933602);国家自然科学基金(51172188);四川省科技支撑计划(2010FZ0048)

Research on Preparation and Biological Properties of Dense Hydroxyapatite Spheres

LUO Hui-Tao, ZHI Wei, LU Xiong, LI Jin-Yu, FENG Bo, WENG Jie

(Key Laboratory of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China)

Received:2012-01-13 Revised:2012-03-16 Published:2013-01-10 Online:2012-12-20
About author:LUO Hui-Tao. E-mail: luo815923@163.com
Supported by:
National Basic Research Program of China (2012CB933602);National Natural Science Foundation of China (51172188);Science and Technology Pillar Project of Sichuan (2010FZ0048)

摘要/Abstract

摘要：

使用羧甲基壳聚糖(CMCS)和明胶(Gel)作为粘结剂, 以沉淀法制得的纳米羟基磷灰石(HA)浆料为原料, 采用溶胶-凝胶法制备出了粒径分布均匀, 几何形态良好的致密HA球粒. 其孔隙率为4.7%±0.6%, 单粒抗压强度为(8.9±0.4) MPa(颗粒直径为0.5 mm), 高于孔隙率为16.4%±0.5%的多孔球的抗压强度(7.9±0.2) MPa。仿生矿化和细胞培养的结果显示致密HA球粒具有良好的生物学性能。

关键词: 羟基磷灰石, 溶胶-凝胶法, 高强度, 致密度, 生物学性能

Abstract:

By using carboxy methyl chitosan (CMCS) and gelatin (Gel) as adhesive, the nano-HA slurry which was fabricated via a precipitation process as the raw material, dense HA spheres with a uniform size distribution and good geometry were prepared successfully by means of Sol-Gel method. Dense HA spheres with a porosity of 4.7%±0.6% have a higher compressive strength (8.9±0.4) MPa than that of porous HA spheres (7.9±0.2) MPa with a porosity of 16.4%±0.5% (particles with diameter of 0.5 mm). The results of biomimetic mineralization and cell culture show that dense HA spheres have an excellent biological property.

Key words: hydroxyapatite, Sol-Gel method, high strength, density, biological property

中图分类号:

R318
TQ174

罗会涛, 智伟, 鲁雄, 李金雨, 冯波, 翁杰. 致密羟基磷灰石球粒的制备及其生物学性能研究[J]. 无机材料学报, 2013, 28(1): 40-44.

LUO Hui-Tao, ZHI Wei, LU Xiong, LI Jin-Yu, FENG Bo, WENG Jie. Research on Preparation and Biological Properties of Dense Hydroxyapatite Spheres[J]. Journal of Inorganic Materials, 2013, 28(1): 40-44.

图/表 4

图1 (a)初始HA浆料TEM照片, (b)HA球粒烧结前后XRD图谱, (c)致密HA球粒光学照片, (d)致密HA球粒SEM照片和(e)HA球粒烧结工艺曲线

Fig. 1 (a) TEM image of initial HA slurry, (b) XRD patterns of initial HA and as-sintered HA spheres, (c) Optical picture of dense HA spheres, (d) SEM image of dense HA spheres and (e) Sintering curve of HA spheres

图2 三种不同HA含量制备的球粒表面的SEM照片

Fig. 2 Surface SEM images of three types of HA spheres

Table 1 Properties of spheres with different HA contents

HA	Porosity/%	Shrinkage/%	Compressive strength/MPa
10wt%	16.4±0.5	58.7±0.7	7.9±0.2
20wt%	8.3±0.4	57.9±0.6	8.6±0.2
25wt%	4.7±0.6	57.4±0.9	8.9±0.4

图3 (a)矿化3 d, (b)矿化5 d, (c)细胞培养3 d后致密HA球粒表面的SEM照片

Fig. 3 SEM images of the surface of dense spheres (a) mineralized for 3 d, (b) mineralized for 5 d and (c) cultured for 3 d

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致密羟基磷灰石球粒的制备及其生物学性能研究

Research on Preparation and Biological Properties of Dense Hydroxyapatite Spheres

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