Journal of Inorganic Materials ›› 2020, Vol. 35 ›› Issue (1): 131-138.DOI: 10.15541/jim20190139

Special Issue: MAX相和MXene材料 副主编黄庆研究员专辑 MXene材料专辑(2020~2021) 【虚拟专辑】层状MAX,MXene及其他二维材料

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Enzyme-MXene Nanosheets: Fabrication and Application in Electrochemical Detection of H2O2

MA Bao-Kai1,2,3,LI Mian3,CHEONG Ling-Zhi2(),WENG Xin-Chu1,SHEN Cai3,HUANG Qing3   

  1. 1. School of Life and Sciences, Shanghai University, Shanghai 200444, China
    2. Department of Food Science and Engineering, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315211, China
    3. Institute of Materials Technology & Engineering, Chinese Academy of Sciences, Ningbo 315201, China
  • Received:2019-03-28 Published:2020-01-20 Online:2019-05-29
  • About author:MA Bao-Kai (1992-), male, Master candidate. E-mail: mabaokai@nimte.ac.cn
  • Supported by:
    Ningbo University(421401560);National Natural Science Foundation of China(21706137);National Natural Science Foundation of China(21671195)

Abstract:

Two-dimensional MXene nanosheets with vertical junction structure was employed for easy immobilization of horse radish peroxidase enzymes to fabricate the electrochemical hydrogen peroxide (H2O2) biosensor. The synthesized MXene nanosheets exhibited large specific area, excellent electronic conductivity and good dispersion in aqueous phase. Horse Radish Peroxidase (HRP) enzymes molecules immobilized on MXene/chitosan/GCE electrode demonstrated good electrocatalytic activity toward reduction of H2O2. The fabricated HRP@MXene/chitosan/GCE biosensor exhibited a wide linear range from 5 to 1650 μmol?L -1, a limit of detection of 0.74 μmol?L -1 and good operation stability. The fabricated biosensor was successfully employed for detection of trace level of H2O2 in both solid and liquid food.

Key words: horse radish peroxidase, MXene nanosheets, biosensor, hydrogen peroxide

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