Preparation of Ag2S Nanocrystals for NIR Photothermal Therapy Application

doi:10.15541/jim20170498

Abstract

Abstract:

Photothermal therapy (PTT) is a promising cancer treatment with both high effectiveness and low side effects. It is very important that the advancement of the NIR photothermal cancer therapy is dependent on the development of photothermal agents. Herein, this study developed hydrophilic DHLA-Ag₂S nanocrystals as a new photothermal agent, which were synthesized by combining a thermal decomposition and ligand exchange route. The as-prepared Ag₂S nanocrystals appear good water solubility, photo thermal stability and biocompatibility. Under irradiation of 980 nm laser with a power density of 5 W/cm², HeLa cancer cells in vitro can be efficiently killed by photothermal effects of the Ag₂S nanocrystals (40 μg/mL). The photothermal effect of Ag₂S nanocrystals and its good fluorescence imaging function can realize the visualization and precision of photothermal therapy.

Key words: Ag₂S nanocrystals, photothermal effect, 980 nm laser, visualization

CLC Number:

O614

DENG Li-Er, LI Yan, GONG Lei, WANG Jia. Preparation of Ag₂S Nanocrystals for NIR Photothermal Therapy Application[J]. Journal of Inorganic Materials, 2018, 33(8): 825-831.

Figures/Tables 8

Fig. 1 Photograph of DHLA-Ag2S nanocrystals aqueous dispersion (a) and XRD pattern (b) with inset showing TEM image of Ag2S nanocrystals

Fig. 2 HRTEM images of the synthesized Ag2S nanocrystals (a) and UV-Vis-NIR spectrum (b) of Ag2S nanocrystals suspended in water

Fig. 3 Temperature elevation of the aqueous dispersion of Ag2S nanocrystals with different concentrations as a function of irradiation time (a) and plot of temperature change (∆T) over a period of 5 min versus the concentration of the Ag2S nanocrystals aqueous dispersion (b)

Fig. 4 Temperature evolution of Ag2S nanocrystals aqueous dispersion (80 μg/mL) during heating (laser on) and cooling (laser off) (a) and linear fitting curve between t and lnθ (b)

Fig. 5 Temperature varied with time of DHLA-Ag2S nanocrystals aqueous solution in three cycles of laser switching

Fig. 6 Cell viability of HeLa cells incubated with different concentrations of Ag2S nanocrystal aqueous solutions for 24 h

Fig. 7 Cell viability of HeLa cells incubated with different concentrations of Ag2S nanomaterials and then being irradiated by a NIR laser for different time

Fig. 8 Fluorescence images of HeLa cells after photothermal treatment with different concentrations of Ag2S nanomaterials under NIR irradiation for 20 min Green fluorecence: live cells; Red fluorescence: dead cells. All bars are 50 μm

References 32

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Preparation of Ag₂S Nanocrystals for NIR Photothermal Therapy Application

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