无机材料学报 ›› 2021, Vol. 36 ›› Issue (2): 181-187.DOI: 10.15541/jim20200131 CSTR: 32189.14.10.15541/jim20200131

所属专题: 【虚拟专辑】药物递送(2020~2021) 【虚拟专辑】抗菌材料(2020~2021)

• 研究论文 • 上一篇    下一篇

负载二甲酸钾缓释抗菌微球的构建

郭小炜1(), 李玉妍1, 陈南春1(), 王秀丽2(), 解庆林3   

  1. 1.桂林理工大学 材料科学与工程学院, 桂林 541004
    2.桂林理工大学 化学与生物工程学院, 桂林 541006
    3.桂林理工大学 广西岩溶地区水污染控制与用水安全保障协同创新中心, 桂林 541006
  • 收稿日期:2020-03-12 修回日期:2020-05-20 出版日期:2021-02-20 网络出版日期:2020-06-09
  • 通讯作者: 陈南春, 教授. E-mail: cnc@glut.edu.cn;
  • 作者简介:郭小炜(1993-), 男, 硕士研究生. E-mail: 781860301@qq.com
  • 基金资助:
    国家自然科学基金(51564008);国家自然科学基金(41662005);广西科技攻关项目(11107003-4);广西自然科学基金(2015GXNSFAA139094);广西自然科学基金(2016GXNSFAA380014)

Construction of Sustainable Release Antimicrobial Microspheres Loaded with Potassium Diformate

GUO Xiaowei1(), LI Yuyan1, CHEN Nanchun1(), WANG Xiuli2(), XIE Qinglin3   

  1. 1. School of Materials Science and Engineering, Guilin University of Technology, Guilin 541004, China
    2. School of Chemical and Biological Engineering, Guilin University of Technology, Guilin 541006, China
    3. Guangxi Collaborative Innovation Center for Water Pollution Control and Water Safety in Karst Area, Guilin University of Technology, Guilin 541006, China
  • Received:2020-03-12 Revised:2020-05-20 Published:2021-02-20 Online:2020-06-09
  • About author:GUO Xiaowei(1993-), male, Master candidate. E-mail: 781860301@qq.com
  • Supported by:
    National Natural Science Foundation of China(51564008);National Natural Science Foundation of China(41662005);Guangxi Science and Technology Project(11107003-4);Natural Science Foundation of Guangxi(2015GXNSFAA139094);Natural Science Foundation of Guangxi(2016GXNSFAA380014)

摘要:

为避免二甲酸钾(KDF)在酸性环境下分解过快, 调节仔猪肠胃道酸碱性和菌落平衡, 实现KDF靶向释放抗菌, 本研究以可生物降解的壳聚糖(CS)、羧甲基纤维素(CMC)和无机刚性材料P型沸石分子筛(Zeolite P)为载体, 负载抗菌药物二甲酸钾制备控释水凝胶微球。发现CS中-NH2与CMC中-COOH离子作用, 可形成结构稳定的聚电解质复合物。溶胀率的差异性表明CS/CMC/Zeolite P水凝胶微球对pH高度敏感。加入Zeolite P使水凝胶微球在pH1.2保持原有形貌且不被降解破裂。CS/CMC/Zeolite P/KDF抗菌微球的包封率为47.75%, 载药率为23.88%, 可有效缓释KDF, 在pH7.4磷酸盐缓冲溶液中的缓释性比pH1.2更优。CS/CMC/Zeolite P/KDF抗菌微球浓度为96 mg/mL时对大肠杆菌最大抑菌率为83%, 有效提高了KDF利用率。

关键词: 二甲酸钾, 水凝胶微球, 缓释, 抗菌

Abstract:

To prevent potassium diformate (KDF) from decomposing too quickly in an acidic environment, adjust the acidity and alkalinity of the gastrointestinal tract of the piglets and the colony balance, and realize the targeted release of KDF antibacterial, in this study, a kind of hydrogel microspheres were prepared by biodegradable chitosan (CS), carboxymethyl cellulose (CMC), and inorganic rigid material P-type molecular sieve (Zeolite P). Then the antibacterial drug potassium diformate was loaded so as to be controlled to release from this hydrogel microsphere. The results show that -NH2 in CS and -COOH in CMC form a stable polyelectrolyte complex through ion interaction. Swelling rate test indicates that the CS/CMC/Zeolite P hydrogel microspheres display high pH-sensitivity. The addition of Zeolite P allows the hydrogel microspheres to maintain their original morphology at pH1.2 without being degraded or broken. The effective sustained-release KDF in pH7.4 phosphate buffer solution exhibits better sustained-release performance than that in pH1.2. When the concentration of CS/CMC/Zeolite P/KDF antimicrobial microspheres is 96 mg/mL, the maximum bacteriostatic rate of Escherichia coli is 83%, which effectively improves the utilization rate of KDF.

Key words: potassium diformate, hydrogel microsphere, sustained-release, antibacterial

中图分类号: