生物玻璃/聚乳酸多孔微球的制备及其作为细胞载体的研究

doi:10.15541/jim20190593

无机材料学报 ›› 2020, Vol. 35 ›› Issue (10): 1163-1168.DOI: 10.15541/jim20190593 CSTR: 32189.14.10.15541/jim20190593

所属专题：封面文章；生物材料论文精选(2020)

生物玻璃/聚乳酸多孔微球的制备及其作为细胞载体的研究

高龙^1,²(),张赵文斌^1,²,常江^1,²()

^1. 中国科学院上海硅酸盐研究所, 上海 200050
^2. 中国科学院大学, 北京 100049

收稿日期:2019-11-23 修回日期:2019-12-19 出版日期:2020-10-20 网络出版日期:2020-01-20
作者简介:高龙(1988-), 男, 博士研究生. E-mail: gaolong@sina.cn。
基金资助:
国家重点研发计划(2016YFC1100201);中国科学院战略性先导科技专项(XDA16010203)

Bioglass/Polylactic Acid Porous Microspheres: Preparation and Their Application as Cell Microcarriers

GAO Long^1,²(),ZHANG Zhaowenbin^1,²,CHANG Jiang^1,²()

^1. Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China
^2. University of Chinese Academy of Sciences, Beijing 100049, China

Received:2019-11-23 Revised:2019-12-19 Published:2020-10-20 Online:2020-01-20
About author:GAO Long (1988-), male, PhD candidate. E-mail: gaolong@sina.cn
Supported by:
National Key Research and Development Plan of China(2016YFC1100201);The Strategic Priority Research Program of the Chinese Academy of Sciences(XDA16010203)

摘要/Abstract

摘要：

具有大孔结构的多孔微球既可以在体外扩增细胞, 还可以作为细胞的传输工具, 通过注射的方式把细胞输送到需要修复的组织部位。生物玻璃虽然生物活性良好, 但难以直接制备成大孔结构的微载体。因此, 本研究将生物玻璃(BG)与聚乳酸(PLA)高分子复合, 通过复乳法制备了一种含生物玻璃的多孔微球细胞微载体。并通过扫描电镜(SEM)、热重分析(TGA)、电感耦合等离子体发射光谱仪(ICP-OES)等方法研究分析了微球的形貌、组成和离子释放。通过细胞实验, 证明细胞可以在微球的多孔结构中粘附和增殖, 并且生物玻璃可以促进细胞增殖, 在组织工程中具有潜在应用。

关键词: 多孔微球, 生物玻璃, 聚乳酸, 细胞微载体, 组织工程

Abstract:

Porous microsphere cell microcarrier with macroporous structure can not only amplify cells in vitro, but also serve as cell delivery tools to deliver cells to damaged tissues by injection. Bioglass (BG) is an inorganic material with excellent biological activity, however, it is difficult to directly prepare microcarriers with macroporous structure. Therefore, in this study, a BG/poly-lactic acid (PLA) porous microspheres was prepared by double emulsion method. The morphology and structure of the microsphere were characterized by SEM, and the load of BG in the microsphere was characterized by thermo gravimetric analysis and its ion release was detected by ICP. Cell proliferation experiments showed that cells could adhere and grow on the surface and inside of the microspheres. The results show that the microsphere with interconnected open-pore microstructure is suit for cell adhesion and proliferation. Bioglass can promote cell proliferation in the microspheres and the obtained BG/PLA composite microsphere has great potential applications in tissue engineering.

Key words: porous microsphere, bioglass, polylactic acid, cell microcarrier, tissue engineering

中图分类号:

R318.08

高龙, 张赵文斌, 常江. 生物玻璃/聚乳酸多孔微球的制备及其作为细胞载体的研究[J]. 无机材料学报, 2020, 35(10): 1163-1168.

GAO Long, ZHANG Zhaowenbin, CHANG Jiang. Bioglass/Polylactic Acid Porous Microspheres: Preparation and Their Application as Cell Microcarriers[J]. Journal of Inorganic Materials, 2020, 35(10): 1163-1168.

图/表 9

图1 生物玻璃/聚乳酸复合微球细胞微载体制备示意图

Fig. 1 Schematic illustration for the preparation of BG/PLA composite microsphere cell microcarriers

图2 PLA浓度为(a) 5.0%、(b) 7.5%、(c) 10.0%、(d) 12.5%的BG/PLA微球的粒径分布

Fig. 2 Particle size distribution of BG/PLA microspheres prepared with PLA solution concentration of (a) 5.0%, (b) 7.5%, (c) 10.0%, and (d) 12.5%

图3 致孔剂用量为(a) 2.0%、(b) 4.0%、(c) 6.0%、(d) 8.0%的BG/PLA多孔微球

Fig. 3 BG/PLA porous microspheres prepared with (a) 2.0%, (b) 4.0%, (c) 6.0%, and (d) 8.0% pore-forming agent

图4 致孔剂用量为(a) 2.0、(b) 4.0、(c) 6.0、(d) 8.0%的BG/PLA微球的孔径分布

Fig. 4 Pore size distribution of BG/PLA microspheres prepared with (a) 2.0, (b) 4.0, (c) 6.0 and (d) 8.0% pore-forming agent

图5 BG/PLA复合微球的热重分析图

Fig. 5 Thermo gravimetric analysis of BG/PLA microspheres

图6 BG/PLA复合微球的Si离子释放

Fig. 6 Si ions released from the BG/PLA microspheres

图7 (a)PLA微球和(b)BG/PLA微球上细胞的活死染色照片

Fig. 7 Live-dead cell staining of cells on (a) PLA microspheres and (b) BG/PLA microspheres

图8 细胞在微球上的增殖

Fig. 8 Proliferation of cells on microspheres

图9 细胞在BG/PLA微球不同截面上的分布

Fig. 9 Distribution of cells on different cross sections of BG/ PLA microspheres

参考文献 17

[1]	MALDA J, FRONDOZA CG . Microcarriers in the engineering of cartilage and bone. Trends in Biotechnology, 2006,24(7):299-304. DOI URL PMID
[2]	R. LANGER, J.P. VACANTI . Tissue engineering, Science. 1993,260(5110):920-926. DOI URL PMID
[3]	ZHOU L, KONG J, ZHUANG Y , et al. Ex vivo expansion of bone marrow mesenchymal stem cells using microcarrier beads in a stirred bioreactor Biotechnology and Bioprocess Engineering, 2013,18(1):173-184.
[4]	QIU QQ, DUCHEYNE P, AYYASWAMY PS . New bioactive, degradable composite microspheres as tissue engineering substrates. Journal of Biomedical Materials Research, 2000,52(1):66-76. DOI URL PMID
[5]	WEI DX, DAO JW, CHEN GQ . A Micro-Ark for Cells: Highly Open Porous Polyhydroxyalkanoate Microspheres as Injectable Scaffolds for Tissue Regeneration. Advanced Materials, 2018,30(31):1802273. DOI URL
[6]	BOO L, SELVARATNAM L, TAI CC , et al. Expansion and preservation of multipotentiality of rabbit bone-marrow derived mesenchymal stem cells in dextran-based microcarrier spin culture. Journal of Materials Science-Materials in Medicine , 2011,22(5):1343-1356. DOI URL
[7]	SU K, GONG Y, WANG C, WANG DA . A novel shell-structure cell microcarrier (SSCM) for cell transplantation and bone regeneration medicine. Pharmaceutical Research, 2011,28(6):1431-1441. DOI URL
[8]	CHUN KW, YOO HS, YOON JJ , et al. Biodegradable PLGA microcarriers for injectable delivery of chondrocytes: Effect of surface modification on cell attachment and function. Biotechnology Progress , 2004,20(6):1797-1801. DOI URL PMID
[9]	YAN S, XIA P, XU S , et al. Nanocomposite porous microcarriers based on Strontium-substituted HA-g-poly (gamma-benzyl-l- glutamate) for bone tissue engineering ACS Applied Materials & Interfaces, 2018,10(19):16270-16281.
[10]	FANG J, YONG Q, ZHANG K , et al. Novel injectable porous poly(γ-benzyl-l-glutamate) microspheres for cartilage tissue engineering: preparation and evaluation. Journal of Materials Chemistry B , 2015,3(6):1020-1031. DOI URL PMID
[11]	FANG J, ZHANG Y, YAN S , et al. Poly (L-glutamic acid)/chitosan polyelectrolyte complex porous microspheres as cell microcarriers for cartilage regeneration. Acta Biomaterialia , 2014,10(1):276-288. DOI URL PMID
[12]	HUANG CC, WEI HJ, YEH YC , et al. Injectable PLGA porous beads cellularized by hAFSCs for cellular cardiomyoplasty. Biomaterials, 2012,33(16):4069-4077. DOI URL PMID
[13]	HENCHL L, XYNOS I, EDGAR A, et al. Preparation and biomineralization research of ultrafine sol-gel bioactive glass power. Journal of Inorganic Materials , 2002,17(5):897-909.
[14]	DING FENG, LI NAN, LI YONG-SHENG , et al. Preparation of hierarchically porous MBG/CPC scaffold and its performance on drug loading and release. Journal of Inorganic Materials, 2013,28(1):97-102.
[15]	ZHOU YAN-LING, FENG XIN-XING, ZHAI WAN-YIN . Study on the loading and releasing behavior of epirubicin hydrochloride from mesoporous bioactive glass (MBGs). Journal of Inorganic Materials, 2011,26(1):68-72.
[16]	MIGUEZ-PACHECO V, HENCH LL, BOCCACCINI AR . Bioactive glasses beyond bone and teeth: emerging applications in contact with soft tissues Acta Biomaterialia, 2015,13(1):1-15.
[17]	MIAO G, CHEN X, DONG H , et al. Investigation of emulsified, acid and acid-alkali catalyzed mesoporous bioactive glass microspheres for bone regeneration and drug delivery. Materials Science & Engineering C-Materials for Biological Applications, 2013,33(7):4236-4243. DOI URL PMID
	BRAUER DS . Bioactive glasses-structure and properties Angewandte Chemie International Edition, 2015,54(14):4160-4181. DOI URL PMID

生物玻璃/聚乳酸多孔微球的制备及其作为细胞载体的研究

Bioglass/Polylactic Acid Porous Microspheres: Preparation and Their Application as Cell Microcarriers

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