KGM/明胶/nano HAP椎间盘纤维环组织工程支架的制备与研究

doi:10.15541/jim20170111

摘要/Abstract

摘要：

椎间盘退变性疾病已经成为严重影响人们工作和生活的疾病, 生物组织工程技术的出现为椎间盘疾病的治疗提供了新的思路和方法。本研究以魔芋葡甘聚糖(Konjac Glucomannan, KGM)、明胶和纳米羟基磷灰石(nano HAP)为原料, 分别采用湿法纺丝法和卷膜法构建了椎间盘纤维环组织工程支架。采用扫描电子显微镜(SEM)、X射线衍射仪(XRD)和傅里叶变换红外光谱仪(FT-IR)对支架的成分、结构和形貌进行了分析, 并测试了纤维环支架的抗压强度、吸水率、孔隙率、体外降解性及细胞毒性。实验结果表明, 得到的椎间盘纤维环组织工程支架具有各向异性的结构特点, 与天然椎间盘纤维环结构极为类似, 且为多孔结构。支架中加入nano HAP可以提高其强度; 湿法纺丝法比卷膜法制备的纤维环支架强度高; 戊二醛交联比氨水交联的纤维环支架强度高, 但降解速率快; 此外, 采用戊二醛作交联剂可对nano HAP进行更好的包裹, 从而更好地提高支架的强度。支架的吸水率均在700%以上, 孔隙率为66%~75%。该研究为研发新型椎间盘纤维环组织工程支架材料提供了一种思路以及一定的实验和理论依据。

关键词: 椎间盘纤维环, 组织工程支架, 魔芋葡甘聚糖, 明胶, 纳米羟基磷灰石

Abstract:

Intervertebral disc degenerative disease has become a serious disease which affects people's work and life, and the emergence of tissue engineering technology provides a new way to the treatment of intervertebral disc disease. In this research, Konjac glucomannan (KGM), gelatin, and nano hydroxyapatite (nano HAP) were used to prepare tissue engineering scaffolds for intervertebral disc annulus fibrosus by wet spinning and rolling film method. Composition, structures and morphologies of the scaffolds were analyzed by scanning electron microscope (SEM), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FT-IR). The compressive strength, water absorption, porosity, in vitro degradation, and cytotoxicity of scaffolds were also measured. The results show that the scaffolds are anisotropic structure, similar to natural annulus fibrosus. The scaffolds are porous, with its strength being improved by adding nano HAP. The strength of scaffolds prepared by wet spinning method are higher than that by rolling film method; the scaffolds cross-linked by glutaraldehyde are stronger than that by ammonia because of better nano HAP combination, and exhibit higher degradation rate. Water absorption and porosity of the scaffolds are over 700%, 66%~75%, respectively. This study provides a theoretical and experimental basis for further development for tissue engineering scaffolds for annulus fibrosus.

Key words: annulus fibrosus, tissue engineering scaffold, konjac glucomannan, gelatin, nano hydroxyapatite

中图分类号:

TB332

陈希亮, 陈庆华, 庄颖, 颜廷亭. KGM/明胶/nano HAP椎间盘纤维环组织工程支架的制备与研究[J]. 无机材料学报, 2018, 33(1): 60-66.

CHEN Xi-Liang, CHEN Qing-Hua, ZHUANG Ying, YAN Ting-Ting. KGM/Gelatin/Nano HAP Scaffolds for Tissue Engineering of Intervertebral Disc Annulus Fibrosus[J]. Journal of Inorganic Materials, 2018, 33(1): 60-66.

图/表 9

图1 纤维环组织工程支架的XRD图谱

Fig. 1 XRD patterns of annulus fibrosus tissue engineering scaffolds^(A) Scaffolds prepared by wet spinning method; (B) Scaffolds prepared by rolling film method

图2 纤维环组织工程支架的红外光谱图

Fig. 2 FT-IR spectra of annulus fibrosus tissue engineering scaffolds^(A) Scaffolds prepared by wet spinning method; (B) Scaffolds prepared by rolling film method

图3 湿法纺丝法制备的纤维环支架的SEM照片

Fig. 3 SEM images of annulus fibrosus scaffolds prepared by wet spinning method^(a, b) Scaffolds crosslinked by ammonia; (c, d) Scaffolds crosslinked by glutaraldehyde

图4 卷膜法制备的纤维环支架的SEM照片

Fig. 4 SEM images of annulus fibrosus scaffolds prepared by rolling film method^(a, b) Scaffolds crosslinked by ammonia; (c, d) Scaffolds crosslinked by glutaraldehyde

图5 纤维环支架的抗压强度

Fig. 5 Compressive strength of annulus fibrosus scaffolds

图6 纤维环支架的吸水率

Fig. 6 Water absorption of annulus fibrosus scaffolds

图7 纤维环支架的孔隙率

Fig. 7 Porosity of annulus fibrosus scaffolds

图8 纤维环支架的降解实验结果

Fig. 8 Degradation results of annulus fibrosus scaffolds

图9 纤维环支架的细胞毒性实验结果

Fig. 9 Cytotoxicity results of annulus fibrosus scaffolds

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