Synthesis of Europium or Terbium Doped Hydroxyapatite Nanorods and Their Cytotoxicity

doi:10.3724/SP.J.1077.2013.12336

Abstract

Abstract:

Using CaCO₃, Eu₂O₃, Tb₇O₄, HNO₃and NH₄H₂PO₄ as starting materials, regulating pH value by NH₃·H₂O, europium-doped and terbium-doped hydroxyapatite (HA:Eu³⁺/ HA:Tb³⁺) nanorods were prepared by hydrothermal method using EDTA as a template. The synthesized materials were systematically characterized by XRD and FESEM. Their luminescence properties and cytotoxicity were studied by fluorescence analysis and MTT assay, respectively. The results show that the main emission peaks of HA:Eu³⁺ and HA:Tb³⁺ nanorods occur at 617 and 544 nm, which can emit bright red and green light, respectively. The cell survival curves show that the prepared HA:Eu³⁺ and HA:Tb³⁺ nanorods can be used as ideal biological tracer materials.

Key words: HA:Eu³⁺, HA:Tb³⁺, EDTA, nanorods, cytotoxicity

CLC Number:

TAO Ting-Ting, WANG Jing-Xian, DONG Xiang-Ting, YU Wen-Sheng, LI Qing-Li. Synthesis of Europium or Terbium Doped Hydroxyapatite Nanorods and Their Cytotoxicity[J]. Journal of Inorganic Materials, 2013, 28(5): 557-560.

Figures/Tables 7

Fig. 1 XRD patterns of HA:Eu3+ and HA:Tb3+ nanorods

Fig. 2 FESEM images of HA:Eu3+(a) and HA:Tb3+(b) nanorods

Fig. 3 Emission and excitation spectra of HA:Eu3+ nanorods

Fig. 4 Emission and excitation spectra of HA:Tb3+ nanorods

Fig. 5 Curves of cell survival rate

References 21

[1]	Sun J J, Bae C J, Koh Y H, et al. Fabrication of hydroxyapatite- poly(epsilon-caprolactone) scaffolds by a combination of the extrusion and bi-axial lamination processes. J. Mater Sci.: Mater. Med., 2007, 18(6): 1017-1023.
[2]	Huang J, Jayasinghe S N, Best S M, et al. Electrospraying of a nano-hydroxyapatite suspension. J. Mater. Sci., 2004, 39(3): 1029-1032.
[3]	Kumar R, Prakash K, Cheang P, et al. Temperature driven morphological changes of chemically precipitated hydroxyapatite nanoparticles. Langmuir, 2004, 20(13): 5196-5200.
[4]	LIAO Jian-Guo, ZHANG Li, ZUO Yi, et al. Surface modification of nano-hydroxyapatite with stearic acid. Chin. J. Inorg. Chem., 2009, 25(7): 1267-1273.
[5]	LIANG Qiong, HAN Dong-Mei, GU Fu-Bo, et al. Synthesis of hydroxyapatite nanorods by hydrothermal recrystallization method. Chin. J. Inorg. Chem., 2007, 23(1): 86-90.
[6]	SUN Yan-Rong, FAN Tao, HUANG Yong, et al. Research trend and prospect of hydroxyapatite bioceramics materials. J. Chin. Ceram. Soc., 2010, 38(6): 1145-1150.
[7]	LI Ling, LIU Yu-Kan, TAO Jin-Hui, et al. Synthesis and characterization of terbium-doped nano-hydroxyapatite biological flurescent probe. Chin. J. Inorg. Chem., 2008, 24(9): 1369-1373.
[8]	Nudelman F, Pieterse K, George A, et al. The role of collagen in bone apatite formation in the presence of hydroxyapatite nucleation inhibitors. Nat. Mater., 2010, 9(12): 1004-1009.
[9]	Peroosa S, Dub Z, Henriette N, et al. A computer modelling study of the uptake, structure and distribution of carbonate defects in hydroxyapatite. Biomaterials, 2006, 27(9): 2150-2161.
[10]	Mendonca G, Mendonca D B S, Aragao F J L, et al. Advancing dental implant surface technology-from micron-to nanotopography. Biomaterials, 2008, 29(28): 3822-3835.
[11]	Yanagida H, Okada M, Masuda M, et al. Cell adhesion and tissue response to hydroxyapatite nanocrystal- coated poly(L-lactic acid) fabric. J. Biosci. Bioeng., 2009, 108(3): 235-243.
[12]	Ren F Z, Xin R L, Ge X, et al. Characterization and structural analysis of zinc-substituted hydroxyapatites. Acta Biomater., 2009, 5(8): 3141-3149.
[13]	Zhang Y Z, Venugopal J R, El-Turki A, et al. Electrospun biomimetic nanocomposite nanofibers of hydroxyapatite/chitosan for bone tissue engineering. Biomater., 2008, 29(32): 4314-4322.
[14]	Zhang H P, Lu X, Leng Y, et al. Molecular dynamics simulations on the interaction between polymers and hydroxyapatite with and without coupling agents. Acta Biomater., 2009, 5(4): 1169-1181.
[15]	Panda R N, Hsieh M F, Chung R J, et al. FTIR, XRD, SEM and solid state NMR investigations of carbonate- containing hydroxyapatite nano-particles synthesized by hydroxide-gel technique. J. Phys. Chem. Solids, 2003, 64(2): 193-199.
[16]	Mao X, Chu C L, Mao Z, et al. The development and identification of constructing tissue engineered bone by seeding osteoblasts from differentiated rat marrow stromal stem cells onto three-dimensional porous nano- hydroxylapatite bone matrix in vitro. Tissue and Cell, 2005, 37(5): 349-357.
[17]	Kino R, Ikoma T, Yunoki S, et al. Preparation and characterization of multilayered hydroxyapatite/silk fibroin film. J. Biosci. Bioeng., 2007, 103(6): 514-520.
[18]	Pelled G, Tai K, Sheyn D, et al. Structural and nanoindentation studies of stem cell-based tissue-engineered bone. J. Biomech., 2007, 40(2): 399-411.
[19]	Cao Chuan-Bao, Liu Si-Yuan, Lǔ Rui-Tao, et al. One step hydrothermal preparation of ZnSe nanorods and their characterization. Transactions of Beijing Institute of Technology, 2004, 24(10): 932-934.
[20]	Wang F K, Cao B R, Mao C B. Bacteriophage bundles with prealigned Ca2+ initiate the oriented nucleation and growth of hydroxyapatite. J. Chem. Mater., 2010, 22(12): 3630-3636.
[21]	Kazuhiko K, Satoko T, Masato W, et al. Effects of modification of calcium hydroxyapatites by trivalent metal ions on the protein adsorption behavior. J. Phys. Chem. B, 2010, 114(7): 2399-2404.