Adsorption and Release Behavior of BSA and FN on Nanostructural Ti Surface

doi:10.3724/SP.J.1077.2011.01299

Journal of Inorganic Materials ›› 2011, Vol. 26 ›› Issue (12): 1299-1303.DOI: 10.3724/SP.J.1077.2011.01299

• Research Paper • Previous Articles Next Articles

Adsorption and Release Behavior of BSA and FN on Nanostructural Ti Surface

SHI Jie, FENG Bo, LU Xiong, WANG Jian-Xin, DUAN Ke, WENG Jie

(Key Laboratory of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China)

Received:2011-01-01 Revised:2011-03-18 Published:2011-12-20 Online:2011-11-11
About author:SHI Jie. E-mail: shilinyue98@126.com
Supported by:
National Natural Science Foundation of China (50871093); Foundation for the Author of National Excellent Doctoral Dissertation of China (200554)

Abstract

Abstract: The titanium dioxide (TiO₂) nanotube arrays with 100 nm diameters on titanium were fabricated by anodic oxidation. The adsorption behaviors of bovine serum albumin (BSA) and fibronectin (FN) onto the nanotube arrays were investigated, and then the protein releases were examined in vitro. Fourier transform infrared spectroscope and Fluorescence microscope were used to qualitative analysis for TiO₂ nanotube arrays surfaces after protein adsorption. Adsorbance of protein was detected with the method of Coomassie brilliant blue G-250. The results showed that TiO₂ nanotube arrays could obviously improve the adsorption, and the adsorption percentage of FN on samples was larger than that of BSA. The release experiment results suggested that the release behaviors of BSA and FN on the nanotube arrays involved the bust release and the gradual release, and the release mechanism was consistent with Fickian diffusion.

Key words: TiO₂ nanotube, protein adsorption and release, bovine serum albumin, fibronectin

CLC Number:

TQ819

SHI Jie, FENG Bo, LU Xiong, WANG Jian-Xin, DUAN Ke, WENG Jie. Adsorption and Release Behavior of BSA and FN on Nanostructural Ti Surface[J]. Journal of Inorganic Materials, 2011, 26(12): 1299-1303.

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Adsorption and Release Behavior of BSA and FN on Nanostructural Ti Surface

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