Structural Properties and Wound Healing Effect of the Sol-Gel Bioactive Glass on Diabetic Skin Wounds

doi:10.3724/SP.J.1077.2013.12232

Abstract

Abstract:

The 45S5 bioactive glass (45S5) was prepared by a melting process, while sol-gel bioactive glass (SGBG) was obtained by Sol-Gel method. The bioactive glasses were characterized by SEM、BET and XRD. Then the wound healing effect was investigated through the wound healing time, wound healing rate and histology examination. The results indicate that the bioactive glass can lessen the wound healing time and increase the healing rates of diabetic rats. Compared with the 45S5, SGBG can promote wound healing of diabetic rats more quickly and efficiently due to the larger surface area and nanostructure. Histological examination shows that bioactive glasses promote the proliferation of fibroblasts and growth of granulation tissue. All results suggest that bioactive glass can accelerate the recovery of skin wounds and SGBG with nanostructure has a better healing effect in diabetes- impaired models.

Key words: bioactive glass, Sol-Gel method, wound healing, diabetes

CLC Number:

TQ174

LIN Cai, MAO Cong, LI Yu-Li, ZHANG Juan-Juan, MIAO Guo-Hou, CHEN Xiao-Feng. Structural Properties and Wound Healing Effect of the Sol-Gel Bioactive Glass on Diabetic Skin Wounds[J]. Journal of Inorganic Materials, 2013, 28(1): 103-108.

Figures/Tables 8

Fig. 1 SEM images of 45S5 (a), SGBG (b)

Fig. 2 XRD patterns of 45S5 and SGBG

Fig. 3 Adsorption-desorption isotherms (a, c) and pore size distributions patterns (b, d) of 45S5 and SGBG Bioglass

Fig. 4 pH values of SBF in different immersion times for two types of the bioglass

Fig.5 Wound healing image following treatments with SGBG, 45S5 and vaseline

Fig. 6 (a) Wound healing times of three groups and (b) Wound healing rates of the diabetic rats treated by different methods (n=8)

Fig. 7 Representative images of H&E staining of wound sections at 7 d and 14 d after surgery. All pictures were taken under 10 times magnification using a Zeiss microscope (bar represents 400 µm). Arrows indicate blood vessels

Table 1 The stages time of granulation tissue formation in different groups (d)

Granulation tissue	Bioactive glass/d	Vaseline
Prophase	0-2	0-4
Early stage	2-4	6-10
Peaking stage	4-6	10-16
Reducing stage	8-12	16-18
Vanishing stage	After 12	After 18

References 20

[1]	Brem Harold, Tomic-canic Marjana. Cellular and molecular basis of wound healing in diabetes. Journal of Clinical Investigation, 2007, 117(5): 1219-1222.
[2]	Hench L L. Bioactive materials: the potential for tissue regeneration. Journal of Biomedical Materials Research, 1998, 41(4): 511-518.
[3]	Hencl L L, Splinter R J, Allen W C, et al. Bonding mechanisms at the interface of ceramic prosthetic materials. Journal of Biomedical Materials Research,1971, 5(6):117-141.
[4]	Gatti A, Valdr G, Andersson H. Analysis of the in vivo reactions of a bioactive glass in soft and hard tissue. Biomaterials,1994, 15(3): 208-212.
[5]	Verrier S, Blaker J J, Hench L L, et al. PDLLA/Bioglass® composites for soft-tissue and hard-tissue engineering: an in vitro cell biology assessment. Biomaterials, 2004, 25(15): 3013-3021.
[6]	Day R M. Bioactive glass stimulates the secretion of angiogenic growth factors and angiogenesis in vitro. Tissue Engeering, 2005, 11(5): 768-777.
[7]	Day R M, Boccaccini A R, Hench L L, et al. Assessment of polyglycolic acid mesh and bioactive glass for soft-tissue engineering scaffolds. Biomaterials, 2004, 25(27): 5857-5966.
[8]	Keshaw H, Forbes A, Day R M. Release of angiogenic growth factors from cells encapsulated in alginate beads with bioactive glass. Biomaterials, 2005, 26(19): 4171-4179.
[9]	Xynos I D, Edgar A J, Buttery L D K, et al. Gene-expression profiling of human osteoblasts following treatment with the ionic products of Bioglass® 45S5 dissolution. Journal of Biomedical Materials Research, 2001, 55(2): 151-157.
[10]	Mortazavi V, Naahrkhalaji M M, Fathi M H, et al. Antibacterial effects of Sol-Gel-derived bioactive glass nanoparticle on aerobic bacteria. Journal of Biomedical Materials Research Part A, 2010, 94A(1):160-168.
[11]	Hench L L. Genetic design of bioactive glass. Journal of the European Ceramic Society, 2009, 29(7): 1257-1265.
[12]	Chen Xiao-feng, Meng Yong-chun, Li Yu-li, et al. Investigation on bio-mineralization of melt and Sol-Gel derived bioactive glasses. Applied Surface Science, 2008, 255(2): 562-564.
[13]	Hench L L, West J K. The Sol-Gel process. Chemical Reviews, 1990, 90(1): 33-72.
[14]	Hench L L. Bioceramics: from concept to clinic. Journal of the American Ceramic Society, 1991, 74(7): 1487-1510.
[15]	Ye Yue-Li, Wang Shi-Hui, Sun Ju-Mei, et al. Healing effect of inorganic elements on second degree burn wounds. Clinical Medicine, 2007, 27(7): 48-49.
[16]	Zhou Lai-sheng, Liao Zhen-jiang, Zhang Qin, et al. Bio-inductive effects of inorganic elements on skin wound healing. Chinese Journal of Burns, 2005, 21(5): 363-366.
[17]	Epstein F H, Singer A J, Clark R A F. Cutaneous wound healing. New England Journal of Medicine, 1999, 341(10): 738-746.
[18]	Blakytny R, Jude E. The molecular biology of chronic wounds and delayed healing in diabetes. Diabetic Medicine, 2006, 23(6): 594-608.
[19]	Maruyama K, Asai J, Li M, et al. Decreased macrophage number and activation lead to reduced lymphatic vessel formation and contribute to impaired diabetic wound healing. The American Journal of Pathology, 2007, 170(4):1178-1191.
[20]	Gurtner G C, Werner S, Barrandon Y, et al. Wound repair and regeneration. Nature, 2008, 453(7193): 314-321.