Bioglass/Polylactic Acid Porous Microspheres: Preparation and Their Application as Cell Microcarriers

doi:10.15541/jim20190593

Abstract

Abstract:

Porous microsphere cell microcarrier with macroporous structure can not only amplify cells in vitro, but also serve as cell delivery tools to deliver cells to damaged tissues by injection. Bioglass (BG) is an inorganic material with excellent biological activity, however, it is difficult to directly prepare microcarriers with macroporous structure. Therefore, in this study, a BG/poly-lactic acid (PLA) porous microspheres was prepared by double emulsion method. The morphology and structure of the microsphere were characterized by SEM, and the load of BG in the microsphere was characterized by thermo gravimetric analysis and its ion release was detected by ICP. Cell proliferation experiments showed that cells could adhere and grow on the surface and inside of the microspheres. The results show that the microsphere with interconnected open-pore microstructure is suit for cell adhesion and proliferation. Bioglass can promote cell proliferation in the microspheres and the obtained BG/PLA composite microsphere has great potential applications in tissue engineering.

Key words: porous microsphere, bioglass, polylactic acid, cell microcarrier, tissue engineering

CLC Number:

R318.08

GAO Long, ZHANG Zhaowenbin, CHANG Jiang. Bioglass/Polylactic Acid Porous Microspheres: Preparation and Their Application as Cell Microcarriers[J]. Journal of Inorganic Materials, 2020, 35(10): 1163-1168.

Figures/Tables 9

Fig. 1 Schematic illustration for the preparation of BG/PLA composite microsphere cell microcarriers

Fig. 2 Particle size distribution of BG/PLA microspheres prepared with PLA solution concentration of (a) 5.0%, (b) 7.5%, (c) 10.0%, and (d) 12.5%

Fig. 3 BG/PLA porous microspheres prepared with (a) 2.0%, (b) 4.0%, (c) 6.0%, and (d) 8.0% pore-forming agent

Fig. 4 Pore size distribution of BG/PLA microspheres prepared with (a) 2.0, (b) 4.0, (c) 6.0 and (d) 8.0% pore-forming agent

Fig. 5 Thermo gravimetric analysis of BG/PLA microspheres

Fig. 6 Si ions released from the BG/PLA microspheres

Fig. 7 Live-dead cell staining of cells on (a) PLA microspheres and (b) BG/PLA microspheres

Fig. 8 Proliferation of cells on microspheres

Fig. 9 Distribution of cells on different cross sections of BG/ PLA microspheres

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