KGM/Gelatin/Nano HAP Scaffolds for Tissue Engineering of Intervertebral Disc Annulus Fibrosus

doi:10.15541/jim20170111

Abstract

Abstract:

Intervertebral disc degenerative disease has become a serious disease which affects people's work and life, and the emergence of tissue engineering technology provides a new way to the treatment of intervertebral disc disease. In this research, Konjac glucomannan (KGM), gelatin, and nano hydroxyapatite (nano HAP) were used to prepare tissue engineering scaffolds for intervertebral disc annulus fibrosus by wet spinning and rolling film method. Composition, structures and morphologies of the scaffolds were analyzed by scanning electron microscope (SEM), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FT-IR). The compressive strength, water absorption, porosity, in vitro degradation, and cytotoxicity of scaffolds were also measured. The results show that the scaffolds are anisotropic structure, similar to natural annulus fibrosus. The scaffolds are porous, with its strength being improved by adding nano HAP. The strength of scaffolds prepared by wet spinning method are higher than that by rolling film method; the scaffolds cross-linked by glutaraldehyde are stronger than that by ammonia because of better nano HAP combination, and exhibit higher degradation rate. Water absorption and porosity of the scaffolds are over 700%, 66%~75%, respectively. This study provides a theoretical and experimental basis for further development for tissue engineering scaffolds for annulus fibrosus.

Key words: annulus fibrosus, tissue engineering scaffold, konjac glucomannan, gelatin, nano hydroxyapatite

CLC Number:

TB332

CHEN Xi-Liang, CHEN Qing-Hua, ZHUANG Ying, YAN Ting-Ting. KGM/Gelatin/Nano HAP Scaffolds for Tissue Engineering of Intervertebral Disc Annulus Fibrosus[J]. Journal of Inorganic Materials, 2018, 33(1): 60-66.

Figures/Tables 9

Fig. 1 XRD patterns of annulus fibrosus tissue engineering scaffolds^(A) Scaffolds prepared by wet spinning method; (B) Scaffolds prepared by rolling film method

Fig. 2 FT-IR spectra of annulus fibrosus tissue engineering scaffolds^(A) Scaffolds prepared by wet spinning method; (B) Scaffolds prepared by rolling film method

Fig. 3 SEM images of annulus fibrosus scaffolds prepared by wet spinning method^(a, b) Scaffolds crosslinked by ammonia; (c, d) Scaffolds crosslinked by glutaraldehyde

Fig. 4 SEM images of annulus fibrosus scaffolds prepared by rolling film method^(a, b) Scaffolds crosslinked by ammonia; (c, d) Scaffolds crosslinked by glutaraldehyde

Fig. 5 Compressive strength of annulus fibrosus scaffolds

Fig. 6 Water absorption of annulus fibrosus scaffolds

Fig. 7 Porosity of annulus fibrosus scaffolds

Fig. 8 Degradation results of annulus fibrosus scaffolds

Fig. 9 Cytotoxicity results of annulus fibrosus scaffolds

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