烧结方式对羟基磷灰石陶瓷颗粒性能和蛋白吸附的影响

doi:10.3724/SP.J.1077.2010.00770

无机材料学报 ›› 2010, Vol. 25 ›› Issue (7): 770-774.DOI: 10.3724/SP.J.1077.2010.00770 CSTR: 32189.14.SP.J.1077.2010.00770

烧结方式对羟基磷灰石陶瓷颗粒性能和蛋白吸附的影响

张慧杰1, 朱向东1, 王辛龙2, 范红松1, 张兴栋1

(四川大学 1. 国家生物医学材料工程技术研究中心; 2. 化学工程学院, 成都 610064)

收稿日期:2009-11-18 修回日期:2010-01-04 出版日期:2010-07-20 网络出版日期:2010-06-10
基金资助:
国家自然科学青年科学基金(50802060); 973计划项目(G2005cb623901)

Effect of Sintering Processes on Surface Properties and Protein Adsorption of Hydroxyapatite Ceramic Particles

ZHANG Hui-Jie¹, ZHU Xiang-Dong¹, WANG Xin-Long², FAN Hong-Song¹, ZHANG Xing-Dong¹

(1. National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, China; 2. School of Chemical Engineering, Sichuan University, Chengdu 610064, China)

Received:2009-11-18 Revised:2010-01-04 Published:2010-07-20 Online:2010-06-10

摘要/Abstract

摘要：

以常规马弗炉和微波烧结两种方式分别制备羟基磷灰石陶瓷颗粒, 对其相组成、微观形貌、表面zeta电位以及蛋白吸附行为进行分析对比. 结果显示, 尽管两种烧结方式制备的羟基磷灰石陶瓷颗粒具有相同的相组成, 但是它们的微观结构、表面zeta电位和蛋白吸附行为存在明显差异. 相对于常规烧结, 微波烧结得到的颗粒具有丰富的微孔隙和接近于纳米尺度的晶粒尺寸, 其表面zeta电位值更小, 能够吸附更多的牛血清白蛋白和更少的溶菌酶. 微波烧结在制备高生物活性纳米羟基磷灰石陶瓷上可能是一种较为理想的方法.

关键词: 羟基磷灰石陶瓷, 常规和微波烧结, 表面特性, 蛋白吸附

Abstract:

Two types of hydroxyapatite (HA) ceramic particles were respectively fabricated by conventional and microwave sintering processes. The conventionally sintered HA was abbreviated as HACS, and the microwave sintered one was HAMS. The phase compositions, surface morphologies and zeta potentials of both particles were respectively analyzed with X-ray diffraction (XRD), scanning electron microscope (SEM) and zetasizer. Bovine serum albumin (BSA) and lysozyme (LSZ) were selected as models to study their adsorption behaviors on HACS and HAMS. Results confirm that both HA particles crystallize completely, but HACS has larger crystal grain size than HAMS. Although both HACS and HAMS show negative surface zeta potentials in phosphate buffered saline (PBS, pH 7.4), the former has higher absolute value than the latter. Besides, both HA particles exhibit different adsorption ability for BSA and LSZ, and HACS adsorbs fewer BSA but more LSZ than HAMS. The microwave sintering can be a good method to produce nano-HA ceramics with excellent bioactivity.

Key words: hydroxyapatite ceramics, conventional and microwave sintering, surface property, protein adsorption

中图分类号:

R318

张慧杰, 朱向东, 王辛龙, 范红松, 张兴栋. 烧结方式对羟基磷灰石陶瓷颗粒性能和蛋白吸附的影响[J]. 无机材料学报, 2010, 25(7): 770-774.

ZHANG Hui-Jie, ZHU Xiang-Dong, WANG Xin-Long, FAN Hong-Song, ZHANG Xing-Dong. Effect of Sintering Processes on Surface Properties and Protein Adsorption of Hydroxyapatite Ceramic Particles[J]. Journal of Inorganic Materials, 2010, 25(7): 770-774.

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烧结方式对羟基磷灰石陶瓷颗粒性能和蛋白吸附的影响

Effect of Sintering Processes on Surface Properties and Protein Adsorption of Hydroxyapatite Ceramic Particles

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